Salt-tolerant halomonas strain and application thereof in field of water purification
A technology of Halomonas and strains, applied in the field of environmental microorganisms, can solve problems such as difficulty in exerting advantages and few salt tolerance characteristics
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Embodiment 1
[0037] Example 1. strain screening and performance testing
[0038] 1. Enrichment culture
[0039]Collect sewage from a fermentation plant, absorb 10ml of sewage and transfer to 100ml enrichment medium (ammonium sulfate 0.5g / L, sodium succinate 5.62g / L, Vickers salt 50ml / L, 50g / L sodium chloride, solvent In a 250ml Erlenmeyer flask with water adjusted to pH=7.0), cultivate under the conditions of 30°C and 200r / min for the first enrichment. When the ammonia nitrogen content drops to the lowest level, absorb another 10ml of the primary enrichment solution, add it to a fresh 100ml enrichment medium, and culture at 30°C and 200r / min for the second enrichment. Carry out the third enrichment according to the above enrichment method.
[0040] 2. Primary screening
[0041] The third enrichment solution was diluted to 10 by gradient dilution method -3 、10 -4 、10 -5 and 10 -6 , Take 200 μl of each dilution on a nutrient agar plate, spread it evenly, and then place it upside down ...
Embodiment 2
[0049] Example 2. Detection and Identification of Halomonas AOB-NY01 Strain
[0050] The slant of the AOB-NY01 strain was sequenced by 16S rDNA gene sequence, and the sequencing results were compared in NCBI (https: / / blast.ncbi.nlm.nih.gov / Blast.cgi?PROGRAM=blastn&PAGE_TYP E=BlastSearch&LINK_LOC=blasthome), select The sequence with the highest similarity was used as the species identification result (see the analysis comparison results in Table 2 for details).
[0051] NCBI comparison results of samples in Table 2
[0052] ID No. DNA identification results identities AOB-NY01 Halomonas sp.GT 99%
Embodiment 3
[0053] Example 3. Halomonas tolerance to different environmental conditions
[0054] 1. Growth of Halomonas AOB-NY01 strain at different salinities
[0055] In a sterile environment, inoculate the AOB-NY01 strain into 100ml enriched medium (ammonium sulfate 0.5g / L, sodium succinate 5.62g / L, Vickers salt 50ml / L, 50g / L sodium chloride, solvent be water, adjust pH=7.0) in a 250ml Erlenmeyer flask, cultivate for 48h under 30°C and 200r / min conditions for activation to obtain an activation solution;
[0056] Draw 5 μl of activation solution to inoculate into 100ml of sterilized evaluation medium (ammonium sulfate 0.5g / L, sodium succinate 5.62g / L, Vickers salt 50ml / L, solvent is water, adjust pH=7.0) In a 250ml Erlenmeyer flask, the salinity of the evaluation medium is 0-30%, cultured at 30°C and 200r / min for 24 hours, and the absorbance OD600 is measured respectively (OD600 represents the absorbance value of the solution at 600nm wavelength, which is used to determine Measuring t...
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