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57 results about "Halomonas sp." patented technology

Soil conditioning, fertility-increasing and salt-reducing agent for sulfate-type saline soil and application thereof to sculellaria barbata planting

InactiveCN105038807AReduce Sulfate LevelsReduce total salt contentSoil lifting machinesOther chemical processesAcrylonitrilePhosphogypsum
The invention discloses a soil conditioning, fertility-increasing and salt-reducing agent for sulfate-type saline soil. The soil conditioning, fertility-increasing and salt-reducing agent comprises the following ingredients by mass: 42-50 parts of phosphogypsum, 15-18 parts of fulvic acid, 6-10 parts of straw, 4-11 parts of medical stone, 7-9 parts of bagasse, 10-20 parts of coal ash, 6-9 parts of aluminium sulfate, 3-8 parts of bean cake, 2-3 parts of hawthorn fruit powder, 0.5-0.8 part of a water-retaining agent, 0.6-0.9 part of lauryl ethoxy ammonium sulfate, 10-16 parts of starch-acrylonitrile, 15-18 parts of hollyhock powder, 10-13 parts of fennel powder and 0.01-0.05 part of halomonas halophiles. The invention further discloses application of the soil conditioning, fertility-increasing and salt-reducing agent to sculellaria barbata planting. The soil conditioning, fertility-increasing and salt-reducing agent and the application thereof to sculellaria barbata planting have the benefits that 12 months after sculellaria barbata planting, sulfate in 100 g of soil has the milliequivalent of 0.09-0.13, the available phosphorus content and the organic matter content of soil are both increased, and the soil moisture permeability and the soil water-retaining capacity are improved.
Owner:WEIFANG YOURONG IND

Construction and application of polygene knockout strain of Halomonas sp. TD01

The invention discloses construction and application of a polygene knockout strain of Halomonas sp. TD01. The invention provides a recombinant strain which is obtained by inactivating one or more genes related to metabolic pathways of propionic acid in the Halomonas sp. TD01 used for producing polyhydroxyalkanoate (PHA). The one or more genes related to metabolic pathways of propionic acid are at least one selected from the group consisting of a coding gene for 2-methylcitrate synthetase, a coding gene for PHA digestive enzyme 1, a coding gene for PHA digestive enzyme 2 and a coding gene for PHA digestive enzyme 3. According to results of experiments in the invention, molecular modification is carried out on the Halomonas sp. TD01 to knock out 2-methylcitrate synthetase PrpC and three PHA digestive enzymes so as to obtain the novel recombinant strain, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) with more excellent material performance can be highly efficiently produced by utilizing propionic acid, and the proportion of 3-hydroxyvaleric acid monomers in the produced PHBV and the conversion rate of the substrate propionic acid are substantially improved.
Owner:BLUEPHA CO LTD

Halomonas TF-1 and application thereof

ActiveCN105154367AEOR valueExtend hot wash cycleBacteriaMicroorganism based processesField testsFermentation
The invention discloses halomonas TF-1 and application thereof. The strain of the halomonas TF-1 is obtained from an oil-water well sample of the Shengli Oil Field under the conditions of 37 DEG C temperature and ordinary pressure by using a high-salt culture medium containing 6.0% of NaCl through enrichment culture screening, temperature-resistant salt-resistant performance investigation, repeated screening and passage, is named as halomonas sp.TF-1, and the preservation number of the halomonas TF-1 is CGMCC No.10619. The nutrient culture medium of the halomonas TF-1 is prepared from 0.3 mg/L of glucose, 0.3 mg/L of peptone, 0.3 mg/L of yeast powder, 0.27 mg/L of K2HPO4 and 6 mg/L sodium chloride, and a pH value is 7.0 -7.5. The halomonas TF-1 grows with crude oil as a carbon source, the bacterial concentration is greater than 5*108 per milliliter. The emulsification viscosity reduction rate of fermentation liquid is higher than 90%. The recovery ratio improved in a physical simulation experiment is higher than 15%. The average oil increase amount of microbial single well stimulation is more than 250 tons. An averagely prolonged hot washing period of microbial paraffin control is greater than 120d. Therefore, the halomonas TF-1 can be widely applied to a field test of microbial single well treatment.
Owner:CHINA PETROLEUM & CHEM CORP +1

Halomonas sp. capable of degrading phenols and high-throughput screening method and application thereof

The invention discloses Halomonas sp. capable of degrading phenols and a high-throughput screening method and application thereof. The Halomonas sp. PH4-5 is preserved under CGMCC 10666. A bacterial liquid is cultured with a 48-pore deep-pore board, phenol concentration is measured by 96-pore microplate reader plate spectrophotometry based on 4-amino antipyrine colorimetry, moderately halophilic bacteria efficient in degrading phenols are screened from 43 environmental samples contaminated by high salinity and organics, the Halomonas sp. is separated and verified from an optimal flora E3, and a salt tolerant degrading mechanism of the Halomonas sp. is studied. Studying results show that quick screening of environmental degrading bacteria provides methodological basis and also provides technical support for the treatment of high-salinity phenolic wastewater.
Owner:EAST CHINA UNIV OF SCI & TECH

Method for treating papermaking waste water by constructing artificial flora

InactiveCN101407362AHas the potential to develop applicationsWastewater dilutionWaste water treatment from plant processingBiological water/sewage treatmentFloraControllability
The invention discloses a method for processing paper-making waste water in the way of structuring artificial flora, including selecting more than two strains of Halomonas sp. flora and Bacillus sp. flora respectively for sterile culture to lead OD620nm to reach over 10.0 and then mixing the bacterial liquid by volume rate to structure the artificial flora; inoculating the paper-making waste water of pH being 9-11 and COD being 1,000-140,000mg l <-1> with the structured artificial flora, conducting stationary treatment for a certain time at 35-38 DEG C with intermittent stirring, in the treatment process, sampling at intervals to measure the pH, chroma and COD index of the paper-making waste water and finishing the treatment when the pH reduces to 7.0-8.8. The method is characterized by simple structure of the flora to be structured, obvious effect in direct treatment, strong controllability and repeatability and the like, and has great application prospect in the treatment aspects of waster water such as the control over the paper-making waste water.
Owner:SHANDONG UNIV

Marine aerobic denitrifying halomonas strain HGMN422 and application thereof

The invention discloses a marine aerobic denitrifying halomonas strain which belongs to the Halomonas (Halomonas sp.), wherein the preservation name of the marine aerobic denitrifying halomonas is Halomonas HGMN422; the preservation company of the marine aerobic denitrifying halomonas is the China center for type culture collection; the preservation data of the marine aerobic denitrifying halomonas is on Aug 28th in 2009; and the preservation number of the marine aerobic denitrifying halomonas is CCTCC (China Center for Type Culture Collection) NO.M209186. The strain has high-efficiency denitrifying capability under the aerobic condition, can be used for rapidly and effectively removing nitrite nitrogen (NO2--N) and nitrate nitrogen (NO3--N) in sea polluted by inorganic nitrogen, has no toxic and pathogenic effects on organisms cultured in the sea and has the characteristics of safety, environment protection and high efficiency for removing pollution of high-concentration inorganic nitrogen in the mariculture environment.
Owner:SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI

Method for preparing heavy metal high-efficiency adsorbent by utilizing compound bacterial agent

The invention relates to the technical field of water treatment, and in particular relates to a method for preparing a heavy metal high-efficiency adsorbent by utilizing a compound bacterial agent. The compound bacterial agent comprises marine salt monoucleosis strains Halomonas sp.GHF11 and marine psychrobacter psychrophilus strains Psychrobacter sp .GHF2; the two types of strains are both obtained by separating and purifying sludge liquid which is spit by ruditapes philippinarum; the two types of the strains are compounded and fermented after being subjected to enlarged cultivation respectively; the two types of the strains are synergic to strengthen each other in fermenting and cultivating processes; the activity of the strains is high; the yield of produced exopolysaccharide is high; the exopolysaccharide is separated by dialyzing a fermented fermenting bacteria liquid, removing part of culture medium components, performing centrifuging and performing ethanol precipitation twice, and then is prepared into the heavy metal adsorbent by curing through agar and starch; the heavy metal adsorbent is good in adsorption effect, high in adsorption efficiency and stable in performance, is safe and harmless, and can be directly added into drinking water.
Owner:北京美胜同创科技发展有限公司

Treatment method for radical curing black liquor of straw pulp from alkaline process

This invention provides a method for treating black liquor from alkali-process straw pulp, and the application of the byproduct. The method comprises: sending fresh black liquor from alkali-process straw pulp (pH is 12-13) into an anaerobic bioreactor containing Bacillus licheniformis, Staphylococcus aureus, and Halomonas sp., performing anaerobic biochemical treatment for 10-15 h to obtain active black liquor (pH is 7.5-8.0), aerating, evaporating, concentrating, and spray-granulating to obtain additive for fertilizer and feed, or industrial byproduct of petrochemical industry and power generation industry. Alternatively, the active black liquor can be directly deodored, sterilized by heating, and sprayed onto crops for improving the quality and increasing the yield, or added into feed for breeding chicks and pigs for stimulating the growth and accelerating the breeding speed. Compared with the present technique, the method can eliminate the environmental pollution of black liquor discharged from papermaking industry, and brings profit to medium and small papermaking plants that use straw as the raw material.
Owner:王照华

Halomonas strain H6, composition and application thereof in salt tolerance and growth promoting

The invention relates to a novel strain of Halomonas sp. obtained by isolation and screening, which has the ability to secrete indoleacetic acid and produce ACC deaminase. The strain can grow well under low temperature or high temperature and salt and alkali conditions. The composition of the strain and a microbial fertilizer prepared by a liquid fermentation technique alleviate the salt-alkali stress caused by the salt on the crops and promote the growth of the crop under salt stress. The novel strain or the composition provided by the invention is suitable for special geographical and climatic conditions and improves the physical and chemical properties of the soil and the ability to fertilize, effectively utilizes environmental resources and reduces pesticide pollution. The novel strainor the composition has the characteristics of good effect, short cycle and good environmental compatibility, and has important practical value for solving the current soil salinization.
Owner:THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI +1

Marine-microbe-sourced low-temperature beta-galactosidase, and coding gene thereof and application thereof

The invention relates to the field of marine biotechnology, and provides a marine-microbe-sourced low-temperature beta-galactosidase, which is obtained by fermentation culturing and purification of a marine halomonas sp. strain P6009-1 obtained by the applicant previously. The marine halomonas sp. strain P6009-1 is cultured and separated from seawater in East China Sea areas, and has a collection number of CCTCC No: M2011001. Under a temperature of 0 DEG C, a capacity of the enzyme in catalyzing lactose hydrolysis is 20% of a maximal activity, such that good low-temperature performance is shown. The invention further provides a coding gene of beta-galactosidase, and an application of beta-galactosidase in decomposing milk lactose.
Owner:SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY

Cheap synthesis of biodegradable mulching film from straws and illegal cooking oil by using halomonas sp ZY-1

The invention discloses a method for synthesizing a biodegradable mulching film from straws and illegal cooking oil by using halomonas sp.ZY-1 under an open and non-sterile condition. Straws are treated to obtain a straw supernatant, and an MS culture medium with the volume ratio of the straw supernatant to illegal cooking oil being 35: 1-40: 1 is prepared. In a sequencing batch reactor (SBR), Halomonas sp.ZY-1 is subjected to aerobic culture with the unsterilized straw supernatant and illegal cooking oil MS culture medium to obtain thalli, and intracellular polyester substances are extracted.Polyester substances are further modified to obtain the mulching film with the tensile strength of 16-18 MPa, the mulching film is deeply buried in cultivated land soil with the depth of 20-30 cm, the 35d weight reduction rate is 40%-50%, and the 35d weight reduction rate is 0.3%-0.5% when the mulching film is placed in dry air. Under the condition that halophilic bacteria are not sterilized, wastes are utilized to synthesize the biological mulching film, so that the production cost is reduced to the maximum extent, and technical support is provided for realizing green ecological cycle and agricultural sustainable development.
Owner:NORTHEAST FORESTRY UNIVERSITY

Halomonas with nitrification and denitrification effects and application thereof

The invention belongs to the technical field of nitrogen pollution, and provides halomonas DY-S025GC05 with nitrification and denitrification effects and application of the halomonas DY-S025GC05. TheLatin of the halomonas DY-S025GC05 with nitrification and denitrification effects is Halomonas sp., the strain has been preserved in China General Microbiological Culture Collection Center, the address is Institute of Microbiology, Chinese Academy of Sciences, No.3, Yard 1, West Beichen Road, Chaoyang District, Beijing, the preservation date is May 19th, 2020, and the preservation number is CGMCCNO.19847. The halomonas DY-S025GC05 provided by the invention has double activities of nitrification and denitrification at the same time, and the conversion of nitrification and denitrification functions can be realized by adjusting the oxygen content.
Owner:THE FIRST INST OF OCEANOGRAPHY SOA +1
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