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Halomonas TF-1 and application thereof

A technology of Halomonas, TF-1, applied in the field of microbial biotechnology and environmental biology

Active Publication Date: 2015-12-16
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many strains resistant to high temperature and high salt in the environment, but there are few strains resistant to high temperature and salt and capable of degrading crude oil emulsification in microbial oil recovery, and there are reports on strains applied to high temperature and high salt reservoirs Therefore, in view of the above situation, it is necessary to screen out halophilic bacteria suitable for high-temperature and high-salt reservoir environment and apply them to microbial oil recovery technology

Method used

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  • Halomonas TF-1 and application thereof
  • Halomonas TF-1 and application thereof
  • Halomonas TF-1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The morphological characteristics and physiological and biochemical characteristics of Halomonas TF-1CGMCC No.10619 provided by the present invention.

[0035] Refer to the experimental method of "Bergey's Mannual of Systematic Bacteriology" to detect its Gram staining, cell size and shape, presence or absence of flagella and spores, growth temperature, and catalase, starch hydrolysis, gelatin liquefaction, galactose, xylose, lactose other biochemical characteristics.

[0036] The research results show that if figure 1 As shown, the strain is Gram-positive, short rod-shaped, without perinatal flagella, motile, aerobic, cell size 4-8μm×0.2-0.5μm, growth temperature 30-60℃, optimal The growth temperature is 37°C, the growth pH range is 5-8, the optimum growth pH range is 7-7.5, and the NaCl tolerance is 0-26%, such as figure 2 .

Embodiment 2

[0038] Halomonas TF-1CGMCCNo.10619 culture fermentation provided by the present invention and performance evaluation of fermentation broth

[0039] Fermentation medium: glucose 0.3mg / L, peptone 0.3mg / L, yeast powder 0.3mg / L, K 2 HPO 4 0.27mg / L, sodium chloride 6mg / L, pH value 7.0.

[0040] Fermentation conditions: inoculum size 2%, inoculation age 12h, initial pH 7.0, temperature 37°C, stirring speed 180rpm, pH=7, aeration rate: 1L / min, evaluation after 24h incubation.

[0041] Performance parameter evaluation and results of fermentation broth:

[0042] (1) Evaluation of growth ability with crude oil as the only carbon source

[0043] Halomonas TF-1CGMCCNo.10619 was inoculated in a hydrocarbon medium with crude oil as the only carbon source, cultured with shaking at 37°C and 180 rpm, and samples were taken at different times for bacterial density testing, as shown in image 3 As shown, the experimental results show that the strain grows with crude oil as the sole carbon so...

Embodiment 3

[0057] The Halomonas TF-1CGMCC No.10619 fermented liquid provided by the present invention is applied in single well C of Shengli Oilfield.

[0058] Oil well profile: Formation temperature 60°C, formation water salinity 2.4×10 4 mg / L, the effective thickness of the oil layer is 6.7m, the porosity is 0.30, and the permeability is 551×10 -3 μm 2 .

[0059] Implementation steps: 100m of Halomonas TF-1 fermentation broth 3 Use a pump truck to pass through the annulus of the casing of oil well C at a distance of 8m 3 / h injection rate into the formation, shut down 10d and resume production.

[0060] Test results: After the test was completed, both the liquid production and oil production of the well increased, and the accumulative oil production increased by 280 tons in 6 months.

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Abstract

The invention discloses halomonas TF-1 and application thereof. The strain of the halomonas TF-1 is obtained from an oil-water well sample of the Shengli Oil Field under the conditions of 37 DEG C temperature and ordinary pressure by using a high-salt culture medium containing 6.0% of NaCl through enrichment culture screening, temperature-resistant salt-resistant performance investigation, repeated screening and passage, is named as halomonas sp.TF-1, and the preservation number of the halomonas TF-1 is CGMCC No.10619. The nutrient culture medium of the halomonas TF-1 is prepared from 0.3 mg / L of glucose, 0.3 mg / L of peptone, 0.3 mg / L of yeast powder, 0.27 mg / L of K2HPO4 and 6 mg / L sodium chloride, and a pH value is 7.0 -7.5. The halomonas TF-1 grows with crude oil as a carbon source, the bacterial concentration is greater than 5*108 per milliliter. The emulsification viscosity reduction rate of fermentation liquid is higher than 90%. The recovery ratio improved in a physical simulation experiment is higher than 15%. The average oil increase amount of microbial single well stimulation is more than 250 tons. An averagely prolonged hot washing period of microbial paraffin control is greater than 120d. Therefore, the halomonas TF-1 can be widely applied to a field test of microbial single well treatment.

Description

technical field [0001] The invention belongs to the field of microbial biotechnology and environmental biotechnology, and in particular relates to a strain of Halomonas TF-1 and its application in microbial oil recovery. Background technique [0002] Microbial oil recovery relies on microbial metabolism to produce acid, carbon dioxide or biogas to expand the pores of oil-bearing layers and increase the mobility of crude oil; or to decompose heavy hydrocarbons into more mobile short-chain compounds, and to produce biosurfactants, biopolymers materials to enhance oil recovery. However, the reservoir environment is often characterized by high temperature and high salinity. To carry out microbial oil recovery in these reservoirs, it is necessary to have high-efficiency bacteria strains that are resistant to temperature and salt. There are many strains resistant to high temperature and high salt in the environment, but there are few strains resistant to high temperature and salt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C09K8/582C12R1/01
Inventor 谭晓明冯云宋欣王静林军章徐登霆巴燕徐闯段传慧刘涛
Owner CHINA PETROLEUM & CHEM CORP
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