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Halomonas sp. capable of degrading phenols and high-throughput screening method and application thereof

A medium halophilic bacteria and screening method technology, applied in the moderate halophilic bacteria degrading phenol and its high-throughput screening and application field, can solve the problem of large reagent consumption, monotonous and cumbersome screening methods, and high screening efficiency low level problem

Inactive Publication Date: 2015-11-25
EAST CHINA UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the traditional bacteria screening method is monotonous and cumbersome, the consumption of reagents is large, and the efficiency of bacteria screening is low, so that only a limited number of samples can be processed

Method used

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  • Halomonas sp. capable of degrading phenols and high-throughput screening method and application thereof
  • Halomonas sp. capable of degrading phenols and high-throughput screening method and application thereof
  • Halomonas sp. capable of degrading phenols and high-throughput screening method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0103] 43 environmental samples were collected from 8 environments polluted by high salinity and organic matter (Table 6). First enrich in 125-mL serum bottles according to step (1) of the screening method, and then transfer 3 times to obtain the bacterial population without sediment.

[0104] Table 6 Sample collection information

[0105]

[0106] The 43 cultures (No: A1–H1) were respectively transferred to 48-well deep-well plates according to step (2) of the screening method, and cultured at 30°C and 200rpm for 72h. According to step (3) of the screening method, the remaining phenol concentration in the deep well plate was determined by using a 96-well microplate reader plate spectrophotometric method based on 4-aminoantipyrine colorimetry. Transfer 200 μL of the bacterial solution in the 48-well deep-well plate to a 96-well microplate reader plate, centrifuge the whole 96-well microplate reader plate at 2500×g for 20 min, transfer the supernatant to a new 96-well micro...

Embodiment 2

[0113] Halomonassp.PH4-5 was inoculated into MSM medium containing 500 mg / L phenol and 3%-12% (w / v) sodium chloride to investigate the degradation of phenol.

[0114] Halomonassp.PH4-5 optimal growth and degradation salinity is 5%. After the strain was grown in MSM medium containing 500 mg / L phenol for 68 hours, the phenol removal rates were 96.2%, 99.8% at 3%, 5%, 8%, 10%, and 12% (w / v) NaCl , 98.9%, 94.7% and 86.3% (see image 3 ). The strain has a removal rate of more than 94% for 500mg / L phenol in the salinity range of 3%-10%, and can degrade high-concentration phenol in a wide salinity range, reflecting the advantages of moderate halophilic bacteria. It can be used for biological treatment of high-salt phenolic wastewater.

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Abstract

The invention discloses Halomonas sp. capable of degrading phenols and a high-throughput screening method and application thereof. The Halomonas sp. PH4-5 is preserved under CGMCC 10666. A bacterial liquid is cultured with a 48-pore deep-pore board, phenol concentration is measured by 96-pore microplate reader plate spectrophotometry based on 4-amino antipyrine colorimetry, moderately halophilic bacteria efficient in degrading phenols are screened from 43 environmental samples contaminated by high salinity and organics, the Halomonas sp. is separated and verified from an optimal flora E3, and a salt tolerant degrading mechanism of the Halomonas sp. is studied. Studying results show that quick screening of environmental degrading bacteria provides methodological basis and also provides technical support for the treatment of high-salinity phenolic wastewater.

Description

technical field [0001] The invention relates to a moderately halophilic bacterium degrading phenol and its screening method and application. Background technique [0002] Wastewater discharged from industries such as petroleum coking, pharmaceuticals, chemicals, printing and dyeing, pesticides, and coal processing contains a large amount of phenol. Phenol is highly toxic, so phenolic wastewater must be removed before it is discharged into receiving water bodies. At the same time, this kind of wastewater contains a large amount of inorganic salts, which increases the difficulty of wastewater treatment and has become a difficult problem in the treatment of phenolic wastewater. [0003] Biological treatment of phenol-containing wastewater has a certain effect, but high-salt conditions will hinder the activity of activated sludge metabolic enzymes, resulting in a decrease in the removal rate of organic matter. For the biological treatment of high-salt phenol-containing wastewa...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12Q1/04C02F3/34C12R1/01
Inventor 李辉刘勇弟王晓丽陆志艳陶萍武骁
Owner EAST CHINA UNIV OF SCI & TECH
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