Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing heavy metal adsorbent by utilizing marine salt monoucleosis strains

A technology of Halomonas and heavy metals, applied in the field of water treatment, can solve the problems of low adsorption efficiency of adsorbents and high adsorption efficiency of heavy metal adsorbents, and achieve the effects of stable performance, strong adsorption capacity and high adsorption efficiency

Active Publication Date: 2018-01-12
ZHEJIANG OCEAN UNIV
View PDF4 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problem of low adsorption efficiency of adsorbents prepared by secreting exopolysaccharides from existing microorganisms, the purpose of the present invention is to provide a method for preparing heavy metal adsorbents using strains of the genus Halomonas marine. The selected strains of Halomonas marine Halomonas sp.GHF11 is isolated from the sludge spit out by clams. The exopolysaccharide secreted by this strain has a large specific surface area, rich complexing groups and strong negative charge, and has a stable ability to adsorb heavy metals. The prepared heavy metal adsorbent has high adsorption efficiency

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing heavy metal adsorbent by utilizing marine salt monoucleosis strains
  • Method for preparing heavy metal adsorbent by utilizing marine salt monoucleosis strains

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for preparing a heavy metal adsorbent by using a marine Halomonas strain, including the following steps:

[0032] (1) Inoculate the selected strain of Halomonas sp.GHF11 on a solid culture medium at 25°C for 36 hours, add sterile water to make a strain, and the volume ratio of the sterile water to the solid medium 1:1;

[0033] (2) Inoculate the bacterial seed liquid into the liquid culture medium, the inoculation concentration is 0.6mL bacterial seed liquid / 100mL liquid medium, ferment culture at 25℃ for 3 days, the shaking speed of the shaker is 80r / min, make the fermentation bacterial liquid ;

[0034] (3) Dialysis and centrifugation of the fermentation broth to remove part of the medium components to obtain the supernatant. The specific process is: place the fermentation broth on a rotary evaporator and concentrate it under reduced pressure at 60°C to 10% of the original volume. Put it into a dialysis bag with a molecular weight cut-off of 8000 Daltons and dialy...

Embodiment 2

[0039] A method for preparing a heavy metal adsorbent by using a marine Halomonas strain, including the following steps:

[0040] (1) Inoculate the selected strain of Halomonas sp.GHF11 on a solid medium at 28°C for 42 hours, add sterile water to make a strain, and the volume ratio of sterile water to solid medium 1.25:1;

[0041] (2) Inoculate the bacterial seed liquid into the liquid culture medium, the inoculation concentration is 0.8mL bacterial seed liquid / 100mL liquid medium, ferment culture at 28℃ for 4 days, the shaker vibration speed is 130r / min, make the fermentation bacterial liquid ;

[0042] (3) Dialysis the fermentation broth to remove part of the medium components and centrifuge to obtain the supernatant. The specific process is: place the fermentation broth on a rotary evaporator and concentrate it under reduced pressure at 62°C to 12% of the original volume. Put it into a dialysis bag with a molecular weight cut-off of 8000 Daltons and dialyze with deionized water ...

Embodiment 3

[0047] A method for preparing a heavy metal adsorbent by using a marine Halomonas strain, including the following steps:

[0048] (1) Inoculate the selected strain of Halomonas sp.GHF11 on the solid medium at 30°C for 48 hours, add sterile water to make the strain, and the volume ratio of the sterile water to the solid medium 1.5:1;

[0049] (2) Inoculate the bacterial seed liquid into the liquid culture medium, the inoculation concentration is 1.0mL bacterial seed liquid / 100mL liquid medium, ferment culture at 30℃ for 5 days, the shaking speed of the shaker is 150r / min, make the fermentation bacterial liquid ;

[0050] (3) Dialysis and centrifugation of the fermentation broth to remove part of the medium components to obtain the supernatant. The specific process is: place the fermentation broth on a rotary evaporator and concentrate it under reduced pressure at 65°C to 15% of the original volume. Put it into a dialysis bag with a molecular weight cut-off of 8000 Daltons and dialyz...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of water treatment, and in particular relates to a method for preparing a heavy metal adsorbent by utilizing marine salt monoucleosis strains. The method comprises the following steps: performing enlarged cultivation and fermentation cultivation on the marine salt monoucleosis strains Halomonas sp.GHF11 to prepare fermented bacteria liquid; then, removing thalli from the fermented bacteria liquid, and performing centrifuging treatment to obtain supernatant; precipitating on two sides through ethyl alcohol to obtain high-purity exopolysaccharide; mixing the exopolysaccharide with agar and starch to obtain the heavy metal adsorbent. According to the method provided by the invention, the exopolysaccharide is produced by screening the marine salt monoucleosis strains Halomonas sp.GHF11; the adsorption capacity of the obtained exopolysaccharide is high; the exopolysaccharide is solidified through the agar and the starch to obtain the heavy metaladsorbent which can be directly added into drinking water; the heavy metal adsorbent is high in heavy metal adsorption efficiency and stable in performance, and is safe and harmless.

Description

Technical field [0001] The invention relates to the technical field of water treatment, in particular to a method for preparing a heavy metal adsorbent by using a marine Halomonas strain. Background technique [0002] Most of the water used by people in production and life is tap water, which is mainly obtained through the treatment of water extracted from water sources such as groundwater or river water through tap water plants. Some water sources are affected by industrial or domestic sewage discharge, which leads to heavy metal content exceeding the standard. Although it is treated by a water plant before being used for human and livestock drinking, the content of some heavy metals is still at a high index, and some even exceed the standard; in addition, urban tap water pipelines Old age can also cause heavy metals such as lead to penetrate into the water body and cause heavy metal content to exceed the standard; although water purifiers with heavy metal removal function are s...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/04B01J20/24B01J20/30C02F1/28C12R1/01C02F101/20
Inventor 穆军穆思源崔霞
Owner ZHEJIANG OCEAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com