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Halomonas 100-16-2 and method for preparing poly-3-hydroxybutyrate with same

A technology of hydroxybutyrate and Halomonas, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problem of unsatisfactory PHB content and so on

Active Publication Date: 2019-03-01
TIANJIN UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The content of PHB obtained is not very ideal

Method used

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  • Halomonas 100-16-2 and method for preparing poly-3-hydroxybutyrate with same
  • Halomonas 100-16-2 and method for preparing poly-3-hydroxybutyrate with same
  • Halomonas 100-16-2 and method for preparing poly-3-hydroxybutyrate with same

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Experimental program
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Effect test

Embodiment 1

[0080] Thalline PHB accumulation expands fermentation culture (5000L fermentation tank), adopts the following steps:

[0081] Seed liquid preparation: take 200 μL of Halomonas 100-16-2 strains stored in glycerol tubes, place them in 5 mL sterile CM medium test tubes, and activate them for 2 days (37°C, 150 r / min) to obtain activation liquid;

[0082] Add 300 mL of sterilized CM medium to a 500 mL Erlenmeyer flask, add 3 mL of activation solution, and expand the culture in a shaker for 3 days (37 ° C, 150 r / min) to obtain the first seed solution;

[0083] Sterilize with 30L CM medium in a 50L fermenter, add 3L of the first seed solution, DO level is 10%, pH 7.5, cultivate for 24h, and obtain the second seed solution;

[0084] Transfer 30 L of the second seed liquid into a 500 L fermenter (300 LCM culture medium, the same culture conditions as the previous step) to continue culturing for 24 hours to obtain the third seed liquid.

[0085] Sterilize with 3000L CM medium in a 5000...

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Abstract

The invention provides a halomonas 100-16-2 and method for preparing poly-3-hydroxybutyrate with the same and relates to technical field of production of poly-3-hydroxybutyrate. The halomonas 100-16-2has a Latin name of Halomonas sp. with a preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 13730. Through gradually amplified cultivation, the halomonas 100-16-2 finally achieves fermentation at 2000-4000 L, and after fermentation is completed, the content of poly-3-hydroxybutyrate is 80% of the dry weight of stain cells, and the dry weight of fermented bacteria is 30 g / L.

Description

technical field [0001] The invention relates to the technical field of poly-3-hydroxybutyrate production, in particular to a method for preparing poly-3-hydroxybutyrate by a strain of Halomonas 100-16-2 and Halomonas 100-16-2. Background technique [0002] Poly 3-hydroxybutyrate (PHB) is a fat-soluble granular substance with a certain particle size. Prokaryotes accumulate a large amount of PHB in their cells when nitrogen is deficient or carbon is excess. Due to its low solubility and relatively high molar mass, PHB can be stored in large quantities in bacterial cells without affecting the osmotic pressure inside and outside the cells. PHB has good biodegradability, and its decomposition products can all be used biologically without any pollution to the environment. It is an ideal storage material. As a microbial synthetic plastic, PHB not only has the characteristics of chemically synthesized plastics, but also has high density, good optical activity, low oxygen permeabili...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P7/62C12R1/01
CPCC12P7/625C12N1/205C12R2001/01
Inventor 隋丽英薄振兴高美荣王振乾
Owner TIANJIN UNIV OF SCI & TECH
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