175results about How to "Improve transformation efficiency" patented technology

Medical devices and related methods for making and using same suitable for treating or inhibiting restenosis are proved. Specifically, compositions and methods for I kappa B alpha (IkBα)nuclear factor kβ (NFkβ) complex breakdown inhibition are provided. One embodiment includes a CRM-1 protein binding composition such as leptomycin B. Another embodiment includes a combination of a CRM-1 protein binding composition and a nucleic acid encoding for mammalian IkBα. Medical devices disclosed include catheters and vascular stents.

In a method for manufacturing a quartz crystal unit, a quartz crystal tuning fork resonator is formed by etching a quartz crystal wafer to form a quartz crystal tuning fork base, quartz crystal tuning fork tines connected to the quartz crystal tuning fork base, and a groove having stepped portions in at least one of opposite main surfaces of each of the quartz crystal tuning fork tines. A first electrode is disposed on at least one of the stepped portions of each of the grooves and a second electrode is disposed on each of side surfaces of each of the quartz crystal tuning fork tines. A frequency of oscillation of the quartz crystal tuning fork resonator is adjusted at least twice and in different steps. The quartz crystal tuning fork resonator is then mounted in a case and an open end of the case is covered with a lid.

An electronic apparatus comprises a display portion and a plurality of oscillators, one of which is a quartz crystal oscillator comprising: a quartz crystal oscillating circuit comprising; an amplification circuit and a feedback circuit. For example, the feedback circuit is constructed by a quartz crystal resonator comprising vibrational arms and a base portion, and having novel shape and electrode construction. Also, the quartz crystal resonator is housed in a package. In addition, from a relationship of an amplification rate of the amplification circuit and a feedback rate of the feedback circuit, an output signal of the quartz crystal oscillating circuit having an oscillation frequency of a fundamental mode of vibration for the quartz crystal resonator can be provided with a frequency of high stability.

An apparatus for electroporation stores a level of charge, specified by a user, on a capacitor, which is delivered to a cuvette through an optically isolated high voltage switch. The capacitor is charged through a charging system, including a current mode pulse width modulation control circuit, which monitors the current in the primary winding of a transformer and supplies a pulse width modulated signal, limiting the current on every pulse to a level set by the microcontroller, to the controlling transistor in order to generate the drive to the primary winding of the transformer. A controlled amount of energy is transferred through each pulse to the capacitor. The microcontroller monitors the voltage on the capacitor up to a threshold level to predict the number of pulses necessary to store the requested amount of charge on the capacitor. The microcontroller will then count the number of pulses until the number of pulses necessary to store the requested amount of charge on the capacitor has been reached. At this point the requested voltage is delivered to the cuvette through the optically isolated high voltage switch comprised of sensitive gate SCRs coupled together in series. The voltage and current at the cuvette are monitored and input to the microcontroller so that the impedance at the cuvette is calculated by the microcontroller and errors in the composition of the solution within the cuvette are detected.

Methods and compositions for modulating plant development are provided. Nucleotide sequences and amino acid sequences encoding Ovule Development Protein 2 (ODP2) proteins are provided. The sequences can be used in a variety of methods including modulating development, developmental pathways, altering oil content in a plant, increasing transformation efficiencies, modulating stress tolerance, and modulating the regenerative capacity of a plant. Transformed plants, plant cells, tissues, and seed are also provided.

The invention discloses a jaw crusher with a single-shaft double-crank-rocker mechanism. The jaw crusher comprises a frame, a fixed jaw plate, a movable jaw plate, a front crank-rocker mechanism, a rear crank-rocker mechanism and a driving wheel, wherein, the front crank-rocker mechanism comprises a front movable jaw, a front toggle plate and an eccentric drive shaft; the rear crank-rocker mechanism comprises a rear movable jaw, a rear toggle plate and an eccentric drive shaft; the front movable jaw and the rear movable jaw are jointly pivoted with the eccentric drive shaft; one end of the front toggle plate is pivoted below the rear side of the front movable jaw, the other end of the front toggle plate is pivoted below the front side of the rear movable jaw, one end of the rear toggle plate is pivoted below the rear side of the rear movable jaw, and the other end of the rear toggle plate is pivoted with the frame; and the movable jaw plate is fixedly connected with the front movable jaw and is opposite to the fixed jaw plate to form a crushing cavity together with the fixed jaw plate. The jaw crusher has the obvious effect that horizontal movement stroke of the lower parts of the movable jaws with the main function of discharging materials is improved to a stroke with a main function of crushing so that one crusher has two-stage crushing efficacy.

A quartz crystal unit comprising a case having an interior space and a mounting portion in the interior space, and a quartz crystal resonator having a base portion and first and second vibrational arms, the quartz crystal resonator being vibratable in a flexural mode, a capacitance ratio r1 of a fundamental mode of vibration of the quartz crystal resonator being less than a capacitance ratio r2 of a second overtone mode of vibration thereof, a mounting arm protruding from the base portion and extending in a common direction with at least one of the first and second vibrational arms, the mounting arm being mounted on the mounting portion of the case, an electrode being disposed on a surface of the mounting arm and a surface in the interior space of the case, the electrode disposed on the surface of the mounting arm being connected to the electrode disposed on the surface in the interior space of the case.

The invention provides a temporary blocking material assisted dragged hydraulic jet reconstruction method for a horizontal well. The method comprises the steps of a, carrying out positive swabbing kick-well-off by using a fracturing fluid; b, carrying out positive swabbing kick-well-off by using a sand-carrying fracturing fluid or a sand-carrying acid fluid; c, carrying out positive swabbing kick-well-off by using a fracturing fluid or an acid fluid; d, turning off a gate of a casing pipe; e, injecting the fracturing fluid from an oil pipe to the stratum, and meanwhile injecting clear water into the casing pipe; f, injecting the fracturing fluid or the acid fluid from the oil pipe to the stratum, and injecting clear water into the casing pipe; g, injecting a carrying fluid containing a temporary blocking material to the stratum, and injecting clear way to the casing pipe after a crack is closed; h, injecting the fracturing fluid to the stratum; and i, when further reconstruction is required, dragging the oil pipe to jet, and repeating the operations from the step a to the step h to finish the reconstruction of the horizontal well. According to the method, the formed crack is blocked temporarily by using the temporary blocking material to ensure that the fluid is converged in the current well section, so that the fluid reconstruction efficiency is increased.

The invention provides a method for adding CO2 and O2 in-ground leaching in heavy carbonic acid type ground water and leachate, which comprises the following steps: (1) adding CO2 and O2 into the leaching solution to leach uranium underground to obtain uranium leachate; (2) adding uranium to the uranium Add CO2 to the leaching solution to adjust the pH value of the uranium leaching solution, and then filter it mechanically; (3) perform ion exchange adsorption on the uranium leaching solution in the adsorption tower; (4) perform leaching on the saturated resin to obtain Cl-type poor resin and leaching qualified solution (5) transforming the Cl-type poor resin with the adsorption tail liquid; (6) acidifying the eluting qualified liquid, adding alkali and aging precipitation at room temperature, washing, filtering and drying the precipitated slurry to obtain uranium products. The method of the invention only adds O2 and CO2 without adding other reagents, which will not cause blockage of ore beds and pollution of underground water; at the same time, the preparation process is integrated, the operation is simplified, and the cost is saved.

Applicants have devised a highly effective, convenient, and expeditious genetic transformation system for filamentous fungi, such as Agaricus bisporus. The preferred method uses an Agrobacterium-mediated transformation protocol. The critical features of this protocol include co-cultivation of the bacterium with fruit body tissue instead of spores. In a preferred embodiment, even higher transformation efficiencies were observed with the use of a homologous promoter in the polynucleotide expression construct in order to drive gene expression.

The invention discloses a multi-port multi-mode IoT communication terminal supporting multi-meter combined reading and a reading method thereof. A wireless communication unit and a data acquisition unit of the communication terminal are respectively connected to a data processing unit; a configuration interface is respectively connected to the wireless communication unit, the data processing unitand the data acquisition unit through a configuration unit; one end of an RS485 interface and / or an RS232 interface is connected to the data acquisition unit, and the other end is used for connectinga plurality of meters; the wireless communication unit includes a LoRa wireless communication subunit and / or a NB-IoT wireless communication subunit and / or a WIFI wireless communication subunit and / ora GPRS communication subunit. The terminal has the advantage that different types and different protocols of sensor or meter reading data are connected to a wireless network in different modes quickly by configuring the multi-port multi-mode IoT communication terminal, thereby reducing the research and development cost, improving the transformation efficiency of the old equipment and reducing theconstruction quantity.

In a unit comprising a quartz crystal resonator, a case having a mounting portion, and a lid connected to the case, the quartz crystal resonator having a base portion, and first and second vibrational arms connected to the base portion, at least one groove being formed in at least one of opposite main surfaces of each of the first and second vibrational arms, at least one mounting arm being connected to the base portion having a length L1 less than 0.5 mm and extending in a common direction with at least one of the first and second vibrational arms, the at least one mounting arm being mounted on the mounting portion of the case.

A unit comprising a quartz crystal resonator vibratable in a flexural mode and having a base portion including a length less than 0.5 mm, and first and second vibrational arms connected to the base portion, at least one groove being formed in each of two of opposite main surfaces of each of the first and second vibrational arms, and an electrode being disposed on a surface of the at least one groove formed in each of the two of the opposite main surfaces of each of the first and second vibrational arms so that the electrode disposed on the surface of the at least one groove formed in each of the two of the opposite main surfaces of the first vibrational arm has an electrical polarity opposite to an electrical polarity of the electrode disposed on the surface of the at least one groove formed in each of the two of the opposite main surfaces of the second vibrational arm.

The invention discloses a low-cost commissioning method for an existing large-scale public building air conditioner system, which is mainly used for commissioning of a ground source heat pumping unit.The low-cost commissioning method comprises the following steps of step (1), constructing an air conditioner set failure diagnosing model; step (2), constructing an air conditioner system load estimating model; and step (3), constructing an air conditioner system optimization model. The invention mainly aims to complete preliminary accommodation of a building air conditioner system in a short time through information as little as possible. According to the method, three models are respectively used for three stages of diagnosis, load pre-estimating and optimization target calculation on a thebuilding air conditioner system.

The present invention relates to plant biotechnology and specifically to a method for genetically transforming Camelina sativa with Agrobacterium-mediated transformation system. It comprises Camelina sativa for producing homologous and heterologous recombinant products including oil and protein products and assessing and screening the efficacy of plant transformation. Also disclosed are transgenic Camelina sativa plants, seeds as well as cells, cell-lines and tissue of Camelina sativa.

The present invention relates to an improved transformation and regeneration system for wheat. In particular, the invention relates to enhancements in the glyphosate selection system. The transformation method is efficient and reliable for production of fertile plants with improved agronomic qualities.

The present invention provides a method for Agrobacterium-mediated gene introduction into a plant material, comprising: 1) treating the plant material, and then 2) infecting the plant material with an Agrobacterium, characterized in that a medium enriched in a metal salt containing copper ion is used in step 1) and / or 2). The present invention also provides a process for preparing a transformed plant characterized in that the gene introduction method of the present invention is used.

The invention discloses an accompanying tracking system for a robot. The accompanying tracking system for the robot is high in automation level and low in cost. The accompanying tracking system for the robot comprises a production line, a robot device and an off-line accompanying device. The robot device comprises a robot walking mechanism, a robot and a handle, wherein the robot walking mechanism is arranged on the side of the production line, the robot is installed on the robot walking mechanism, and the handle is installed on the robot. The handle comprises a base, a handle body, a pair of laser sensors, a 2-dimensional camera and a camera light source. The off-line accompanying device comprises a truss, a pair of guide rails, an accompanying plate, a first belt mechanism, a second belt mechanism, a motor, an encoder, a belt clamping mechanism and a long pin telescoping mechanism. When the belt clamping mechanism clamps a belt of the first belt mechanism, the long pin telescoping mechanism and a belt of the second belt mechanism move along with the belt of the first belt mechanism; when the belt clamping mechanism loosens the belt of the first belt mechanism, the long pin telescoping mechanism and the belt of the second belt mechanism are pushed by workpieces moving on the production line to move.

The embodiment of the invention discloses a design method for coal reservoir transformation, and belongs to the field of coalbed methane exploration. The design method comprises the following steps: acquiring the thickness, the effective porosity, the phase change transformation radius and the phase change transformation temperature of a coal reservoir, acquiring a shaft heat loss efficiency of an injection well, confirming a phase change fluid amount and a partitioning fluid amount according to the phase change transformation radius and the effective porosity of the coal reservoir, confirming an injection delivery capacity and the highest pressure of the opening of the injection well when fluid is injected into the coal reservoir according to fracture pressure of the coal reservoir, confirming a cold amount for phase change transformation coal reservoir according to the phase change fluid amount, the specific heat capacity of a pore medium in the coal reservoir, and the density and the phase change transformation temperature of the pore medium in the coal reservoir, confirming a refrigerant amount and a cold source amount according to the shaft heat loss efficiency, the specific heat capacity of a refrigerant liquid and the temperature of the refrigerant liquid after heat exchange with the ground, and transforming the coal reservoir according to phase change of the phase change fluid. By adoption of the design method, the coal reservoir transformation efficiency can be improved.

The present invention relates to a method of introducing gene into plant material via Agrobacterium. A method of the present invention is characterized in that it comprises: 1) pressurizing a plant material, and then 2) infecting the plant material with an Agrobacterium.

The invention discloses a method and device for switching multiple ONU authentication modes in a PON system, and relates to the field of ONU management in PON systems. The method comprises the steps that a PON opening authentication mode is changed into a mixed authentication mode, and an ONU authentication mode is changed into a logic information authentication mode; a legal ONU logic information white list is configured, ONU logic information and ONU physical address information are associated through an OLT according to the logic information white list; an ONU is accessed and is authenticated through the OLT based on the logic information white list; after ONU authentication is successful, the OLT sends ONU authorization and business configuration information to a business interface disc. By means of the method and device for switching the multiple ONU authentication modes in the PON system, the various ONU authentication modes can be switched smoothly, operation process is simpler, and project reconstruction cost can be reduced.

The invention relates to a system for cutting and connecting circuit, which comprises user terminal, network unit, and a network manager work station. Wherein, it based on the circuit cutting-connecting request of user terminal calculates and preforms the circuit route and builds the preformed circuit; based on the preformed circuit and route, generates the service processed at cutting and connecting circuit, and sending the service to the network unit. The invention also disclose a relative method which comprises: based on the circuit cutting-connecting request of user terminal calculates and preforms the circuit route and builds the preformed circuit; based on the preformed circuit and route, generates the service processed at cutting and connecting circuit, and sends the service to the network unit, then cuts and connects the circuit at the network unit. With said invention, user can change present network at network manager work station, to redistribute the network resource, to improve the efficiency.

The embodiment of the invention discloses a digitalized transformation method for a transformer substation. The digitalized transformation method comprises an original bus protection device upgrade step, a total-interval sub-machine analog quantity and IO quantity conversion step, a total-interval sub-machine data transmission step, a step of converting GOOSE receiving quantity into IO quantity, a duty-cycling control interval transformation step and a total-interval sub-machine exit step; after an entire station SCD file is configured, the SCD file is subjected to privatization configuration and then is unloaded into a total-interval sub-machine; associated configuration information of all untransformed intervals required for a digitalized bus difference is modified to be completely transmitted by the total-interval sub-machine; meanwhile, a virtual terminal of requiring conventional tripping operation in digitalized bus difference GOOSE tripping information is associated into the total-interval sub-machine for opening; and a fiber connecting line only needs to be modified to be connected to an intelligent terminal and a merging unit (MU) corresponding to the terminal by the total-interval sub-machine when each interval is transformed each time, so that seamless switching of the total-interval sub-machine is achieved; and the digitalized transformation method has the technical effects of small boundedness, high operability, safety, reliability, simplicity in construction and high transformation efficiency.

The invention provides a method and a device for processing unified login of mobile applications. The method comprises the following steps: packaging one or more unified login mobile applications; establishing a unified login entry for the one or more login and packaged mobile applications; and carrying out unified login processing on the one or more packaged mobile applications according to unified login information for unified login and the established unified login entry. By adopting the method and the device provided by the invention, for the unified login of the mobile applications in a related technology, the problems of troublesome transformation, complex processing steps and high cost are solved, therefore the effect that one application only needs to input the login information at the first time to achieve unified secure login so as to improve the login efficiency and effectively improve the access efficiency and experience of users is achieved.

A method is disclosed for producing a transgenic cotton plant by Agrobacterium-mediated transformation of petiole tissue. The method comprises the steps of (a) obtaining cotton petiole explants, (b) exposing the petiole explants to a culture of Agrobacterium tumefaciens that harbors a vector comprising an exogenous gene and a selectable marker, the Agrobacterium being capable of effecting the stable transfer of the exogenous gene and selection agent resistance gene to the genome of the cells of the petiole explant, (c) culturing the petiole explants to induce callus formation, (d) selecting transformed callus that expresses the exogenous gene, (e) culturing the selected callus in suspension culture to induce formation of embryoids, (f) regenerating the embryoids into whole transgenic cotton plants.

A nitrogen-containing thermoelectric material, which has an element composition represented by:AlzGayInxMuRvOsNt  (A)orAlzGayInxMuRvDwNm  (B)(wherein M represents a transition element; R represents a rare earth element; D represents at least one element selected from elements of the Group IV or II; 0≦z≦0.7, 0≦y≦0.7, 0.2≦x≦1.0, 0≦u≦0.7, 0≦v≦0.05, 0.9 5≦s+t≦1.7, 0.4≦s≦1.2, 0≦w≦0.2 and 0.9≦m≦1.1; and x+y+z=1), and has an absolute value of a Seebeck coefficient of 40 μV / K or more at a temperature of 100° C. or more. These thermoelectric materials comprise elements having low toxicity, are excellent in a heat resistance, a chemical resistance and the like, and have a high thermoelectric transforming efficiency.

The invention discloses an efficient imbibition and drag-reducing water system applicable to an atmospheric pressure shale gas reservoir. The efficient imbibition and drag-reducing water system applicable to the atmospheric pressure shale gas reservoir comprises the following components: 0.04-0.1 percent of a high-molecular polymer drag reducer, 0.05-0.2 percent of a moisture regulator, 0.1-0.3 percent of an efficient shale inhibitor, 0.01-0.03 percent of an environment-friendly fungicide, 0.05-0.1 percent of an imbibition conditioner and the balance of water. The efficient imbibition and drag-reducing water system applicable to the atmospheric pressure shale gas reservoir has the advantages that the efficient imbibition and drag-reducing water system is prepared by using a low molecular weight high-molecular polymer as a drag reducer and introducing the moisture regulator, the imbibition conditioner, the efficient shale inhibitor and the environment-friendly fungicide. Therefore, therock core spontaneous imbibition amount can be effectively increased, the imbibition of a fracturing fluid to a deep ground mass can be promoted; smoothness of a spontaneous imbibition passage can bekept; the desorption of adsorbed gas can be promoted; effects of water soluble replacement of the adsorbed gas, energy increasing and drainage are achieved; atmospheric pressure shale fracking construction requirements are satisfied; the drainage of atmospheric pressure shale gas drag-reducing water fracturing fluid flow-back fluid is reduced; and the development of economic benefits of an atmospheric pressure shale gas field is realized.

The invention discloses a multi-axis drilling device for aluminum alloy wheel PCD holes, which includes a drill spindle, which includes a spindle pull rod, an upper faceplate, a lower faceplate, a center drill, a first electric spindle and a reinforcing rib, and eight supporting components. It includes the first motor, supporting block, rolling shaft, rotating shaft, lock nut, bearing seat, countersunk screw, ball screw and the first coupling, each supporting assembly has a drill slide, and each rotating shaft is connected to the first The two motors are connected. The invention has the advantages of simple connection, convenient operation and low modification cost, and can drill the center hole of the aluminum alloy wheel hub and up to eight PCD holes at the same time, which improves the processing efficiency, and can automatically change the relative angle and pitch circle diameter of the PCD hole drill bit, reducing the process The production and investment of equipment ensure the quality of PCD holes during porous processing.