52 results about "Camelina sativa" patented technology

The invention relates to a method for planting Camelina sativa in saline-alkali soil, belonging to the technical field of agriculture. The method comprises the following steps: firstly, improving the saline-alkali soil; and secondly, respectively planting the Camelina sativa in the improved saline-alkali soil in spring and autumn. By using the method provided by the invention, the saline-alkali soil is subjected to adaptable modification, so that the Camelina sativa can favorably grow, and the production cost of the Camelina sativa is remarkably reduced. Meanwhile, a production method for planting the Camelina sativa in spring and autumn within one year is adopted for the first time, and the yield of the Camelina sativa is further increased.

The present invention relates to plant biotechnology and specifically to a method for genetically transforming Camelina sativa with Agrobacterium-mediated transformation system. It comprises Camelina sativa for producing homologous and heterologous recombinant products including oil and protein products and assessing and screening the efficacy of plant transformation. Also disclosed are transgenic Camelina sativa plants, seeds as well as cells, cell-lines and tissue of Camelina sativa.

The invention relates to a Camelina sativa (L.) Crantz spring-type seed designated as ‘SO-50’ derived from a cross between camelina accessions with high yield and oil quality attributes following conventional breeding methodologies.

The invention discloses a nitrilase N1 derived from pantoea sp.AS-PWVM4 and a gene thereof, a nitrilase N2 derived from arabidopsis thaliana and a gene thereof, a nitrilase N3 derived from acidovoraxfacilis 72W and a gene thereof, a nitrilase N4 derived from leptolyngbya sp. and a gene thereof, a nitrilase N5 derived from brassica oleracea var.oleracea and a gene thereof and a nitrilase N6 derived from camelina sativa and a gene thereof, and a method for preparing paracyanobenzoic acid as p-aminomethylbenzoic acid intermediate by using the nitrilase as a biological catalyst; resting cells ofthe corresponding nitrilases can be used for catalyzing 100g / L of substrate; the conversion rate is greater than 99%; the method has the obvious characteristics of mild reaction conditions, no pollution and simple process route, and has broad industrial application prospects.

The invention relates to a Camelina sativa (L.) Crantz spring-type seed designated as ‘SO-40’ derived from a cross between camelina accessions with high yield and oil quality attributes following conventional breeding methodologies.

The invention relates to a Camelina sativa (L.) Crantz spring-type seed designated as ‘SO-60’ derived from a cross between camelina accessions with high yield and oil quality attributes following conventional breeding methodologies.

The present invention provides methods for transforming Camelina plants. In particular, the present invention relates to transforming Camelina sativa plants through contacting the plants to a dipping solution comprising Agrobacterium, a sugar, and a nonionic surfactant. The methods do not require a vacuum filtration step. The present invention provides, for example, useful methods for developing transformation systems for Camelina sativa that can enable manipulation of its agronomic qualities.

The present invention relates to plant biotechnology and specifically to a method for genetically transforming Camelina sativa with Agrobacterium-mediated transformation system. It comprises Camelina sativa for producing homologous and heterologous recombinant products including oil and protein products and assessing and screening the efficacy of plant transformation. Also disclosed are transgenic Camelina sativa plants, seeds as well as cells, cell-lines and tissue of Camelina sativa.

The invention discloses a bionic camellia extract-containing composition for repairing skin barrier and application of the composition. The composition is prepared from skin surface lipid film repairing components: squalene substances, fatty acid, triglyceride and cholesterol analogues; keratinocyte interstitial components: free fatty acid, ceramide and cholesterol analogues; synergistic interaction componentcomprises: a relieving and inflammation diminishing component, an anti-aging component, a whitening component and a skin penetrating agent; wherein the skin surface lipid film repairing and keratinocyte interstitial components are prepared from the camellia extract, camelina oil, oat kernel oil, squalane, phytosterol and ceramide 3. According to the composition for repairing the skin barrier, on the basis of the principle of the human skin barrier, keratinocyte interstitial components and skin surface lipid film repairing components which are similar to the human skin barrier in structure are subjected to bionic matching through a large number of formula tests and studies, the components in the skin barrier are safely simulated, and the complete and healthy epidermal barrier isremodeled.

The present invention provides isolated FAD2 and FAE1 genes and FAD2 and FAE1 protein sequences of Camelina species, e.g., Camelina sativa, mutations in Camelina FAD2 and FAE1 genes, and methods of using the same. In addition, methods of altering Camelina seed composition and / or improving Camelina seed oil quality are disclosed. Furthermore, methods of breeding Camelina cultivars and / or other closely related species to produce plants having altered or improved seed oil and / or meal quality are provided.

The invention relates to a Camelina sativa (L.) Crantz spring-type seed designated as ‘SO-40’ derived from a cross between camelina accessions with high yield and oil quality attributes following conventional breeding methodologies.

The present invention relates to breeding oil crop through the process of crossbreeding of Camelina sativa and C. macrocarpa f. longistipata as parents; cultivating the hybrid plumule into plant; culturing pollen haploid plant with the anther of the hybrid plant; chromosome doubling to culture amphiploid plant; and multiple generation selection of amphiploid plant seed with thousand grain weight not lower than 1.0 gram to select stable plant for field cultivation. The seed oil of the present invention contains linolic acid over 18 wt%, alpha-linolenic acid over 33 wt%, Camelina sativa acid over 3.05 wt% and vitamin E over 36 mg.

The invention discloses an edible fungus culture medium. The raw materials of the culture medium include, by weight, 20-40 parts of hard mixed wood chips, 10-15 parts of bagasse, 5-15 parts of bean dregs, 10-30 parts of sodium hydrogen phosphate, 5-15 parts of camelina sativa cake, 5-15 parts of kelp residue, 10-30 parts of cotton seed hulls, 5-10 parts of an ionized calcium solvent, 10-30 parts of outer skin of a bamboo shoot, 5-10 parts of gibberellin, 1-5 parts of vitamins, 2-8 parts of ammonium sulfate, 2-10 parts of yam powder, 2-10 parts of common self-heal fruit-spike, 1-5 parts of ferric citrate, 2-10 parts of silkworm chrysalis powder, 1-5 parts of liquorice root, and 1-6 parts of fermented chicken manure. Through the culture medium, cultivation period can be shortened, yield canbe enhanced, the nutrition raw materials in the medium can be enriched, and full supply of the raw materials needed by strains can be guaranteed; and the culture medium is suitable for the edible fungus culturing of various edible fungi like pleurotus eryngii, flammulina velutipes, pleurotus citrinopileatus, oyster mushroom, pleurotus nebrodensis, shiitake mushroom, auricularia auricula-judae, etc.

This disclosure provides a novel herbicide resistant plant, a method to transform Camelina sativa for herbicide resistance and a method for an improved in vitro regeneration of transformed plants.

This disclosure provides a method to modify seed oil composition of Camelina sativa plants. The disclosure also provides novel promoters and gene sequences for modification of plant seed oil composition.

The invention relates to an expanded sinking feed capable of improving the contents of ALA (Alanine), EPA (Eicosapentaenoic Acid) and DHA (Docosahexaenoic Acid) of salmons and belongs to the technical field of processing of fish feed. The feed provided by the invention contains 15 percent to 39.45 percent of germinated camelina sativa; the germinated camelina sativa is cultured through germinating camelina sativa seeds and the length of buds is 1mm to 5mm. According to the expanded sinking feed provided by the invention, compared with feed which is not added with the germinated camelina sativa, the feeding coefficient is remarkably reduced (the absorption utilization rate is improved), and the contents of the ALA, the EPA and the DHA in the fed salmons are significantly improved. Furthermore the cost of raw materials for preparing the feed is low; no antibiotic or chemical drug are added; the expanded sinking feed has no pollution to the environment; no harmful components are remained in the salmons, the physiological functions of the salmons can be effectively improved, the physical properties of the salmons are enhanced, and the meat yield and the quality of the salmons are improved, so that the expanded sinking feed is applicable to various scales and various types of salmon production.

Provided are plants that express, or overexpress, type III Gγ protein AGG3. Such plants exhibit faster vegetative and reproductive growth, accompanied by an increase in photosynthetic efficiency. Constitutive or seed-specific expression of AGG3 in Camelina increases seed size, seed mass, and seed number per plant by 15-40%, effectively resulting in significantly higher oil yield per plant. AGG3-expressing Camelina plants also exhibit improved stress tolerance. Use of AGG3 is therefore an effective biotechnological tool to dramatically increase stress tolerance and plant yield, including oil, in agricultural and horticultural crops.

The invention discloses a method for determining the contents of eight flavonoids and phenolic acid components in camelina sativa seeds, which comprises the following steps that by virtue of a separation analysis technology of an ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry detector and a diode array detector and by virtue of one-detection and multi-evaluation mehod, an easily available catechin reference substance is taken as an internal reference substance, the relative correction factors between the easily available catechin reference substance and the other seven components adopted, so that the content of the eight flavonoid components in the camelina sativa seeds is measured. The method is easy to operate, high in sensitivity, accurate, efficient and low in cost, multi-index components in the camelina sativa seeds are rapidly and accurately quantified at the same time, the separation degree of the eight components is good, and a theoretical foundation is laid for quality control of the camelina sativa seeds.

The subject invention relates to a Camelina sativa (L.) Crantz spring-type seed designated as “SO-70” derived from a three-way cross between Camelina accessions with high yield and oil quality attributes following conventional breeding methodologies.

The invention discloses a selenium-rice culture medium for edible fungi. Raw materials for the selenium-rice culture medium comprise, by weight, 20-40 parts of hard miscellaneous wood dust, 60-75 parts of tea tree branch residues, 5-15 parts of bean dregs, 10-30 parts of disodium hydrogen phosphate, 5-15 parts of camelina sativa cake, 5-15 parts of kelp residues, 10-30 parts of cottonseed hulls, 5-10 parts of ionized calcium solvents, 10-30 parts of bamboo shoot shells, 5-10 parts of gibberellin, 1-5 parts of vitamin, 2-8 parts of ammonium sulfate, 2-10 parts of rhizoma dioscoreae flour, 2-10parts of spica prunellae, 1-5 parts of iron citrate, 2-10 parts of silkworm pupa meal, 1-5 parts of radix glycyrrhizae and 1-6 parts of fermented chicken manure. The selenium-rice culture medium for the edible fungi has the advantages that the cultivation cycle can be shortened, the yield can be increase, nutritional raw materials in the selenium-rice culture medium can be enriched, accordingly, raw materials required by strains can be assuredly sufficiently supplied, and the selenium-rice culture medium is applicable to culturing the diversified edible fungi such as pleurotus eryngii, flammulina velutipes, pleurotus citrinopileatus, pleurotus ostreatus, pleurotus nebrodensis, lentinus edodes and auricularia auricula.

An oral or injection preparation of the medium and long-chain fat emulsion of Camelina sativa oil relates to a medical care and nutritional fat emulsion product which contains Camelina sativa oil and is prepared according to prescription. The weight proportion of the invention is: 0.1 percent to 20 percent of Camelina sativa oil which is taken as medicine, 0 percent to 20 percent of MCT which is taken as rapid energy-provision agent, 0 percent and 15 percent of lecithin which is taken as emulsifier, 0 percent and 30 percent of refined oil which is taken as oil solvent and 0.15 to 0.25 percent of glycerin which is taken as isoosmotic adjustment agent, and water for injection is added till the total amount reaches 1000g; wherein, the final osmotic pressure of glycerin which is taken as isoosmotic adjustment agent is 0.5 to 3 isotonic concentration. The invention has the advantages that the preparation not only satisfies the fundamental needs of the human body on essential fatty acid, but also can greatly reduce the adverse effects caused by excessive polyunsaturated fatty acid and saturated fatty acid simultaneously. Compared with the common soybean oil fat emulsion, the invention can reduce lipid peroxidation to the utmost degree, improve immunity, increase the level of the active vitamin E Alpha-tocopherol in the human body, enhance the stability of the emulsion and speed up the synthesis of long-chain fatty acids.

A method to provide transgenic doubled haploid plants is provided. The doubled haploid plants of this disclosure express heterologous gene products in their seeds. The disclosure also provides a clonal-like isogenic production system in dicotyledonous plant seeds where transgene expression can be controlled. The disclosure specifically provides a system to produce high quality and quantity of heterologous gene product in doubled haploid Camelina sativa seeds.

The present invention relates to breeding oil crop through the process of crossbreeding of Camelina sativa and C. macrocarpa f. longistipata as parents; cultivating the hybrid plumule into plant; culturing pollen haploid plant with the anther of the hybrid plant; chromosome doubling to culture amphiploid plant; and multiple generation selection of amphiploid plant seed with thousand grain weight not lower than 1.0 gram to select stable plant for field cultivation. The seed oil of the present invention contains linolic acid over 18 wt%, alpha-linolenic acid over 33 wt%, Camelina sativa acid over 3.05 wt% and vitamin E over 36 mg.

The invention provides a composition for relieving and treating eyestrain caused by electronic radiation, and a preparation method and application thereof. The composition is mainly prepared from cameliva sativa extract, an anthocyanin extract, a rhodiola rosea extract, a cassia seed extract, a feather cockscomb seed extract, a mushroom extract and radix rehmanniae extract into different dosage forms and different foods. The composition has the advantages that the raw materials are all natural, easy to obtain and cheap, effective components of natural products are enriched through extraction,and the extraction process is simple and free from pollution; the cameliva sativa extract is extracted by taking squeezed cameliva sativa meal as a raw material, influences of the cameliva sativa mealas garbage on the environment are avoided, the cost of the raw material is greatly reduced, resource recycling is realized, and the composition has a good medicinal effect; products prepared from thecomposition can be presented in various forms, are convenient to use and safe to take, can effectively relieve and treat the eyestrain, resist radiation and improve immunity, and have few clinical side effects but significant social and economic benefits.

The invention discloses an edible fungus culture medium containing camelia sativa straws. The edible fungus culture medium is characterized by comprising the following raw materials in percentage by weight: 40%-55% of camelia sativa straws, 25%-40% of fruit tree wood shavings or broad-leaved tree sawdust and cottonseed hull, 4%-8% of camelia sativa cake meal, 3%-5% of bran, 3%-5% of corn flour or sorghum flour, and 1%-2% of calcium carbonate or calcium superphosphate and lime powder. According to the edible fungus culture medium, the rich nutrition in the camelia sativa straws can be reasonably and effectively utilized; the nutrition values of the cultured edible funguses are further improved; the edible funguses are relatively high in nutrition value and relatively rich in content of nutrient; the resource of the nutrition-enriched camelia sativa straws can be reasonably utilized; the combination of the camelia sativa straws and other auxiliary material types can be flexibly adjusted according to the types of the edible funguses so as to meet the growth conditions of different types of the edible funguses, so that the growth conditions of the edible funguses are optimal. The invention also provides a preparation method of the culture medium. The preparation method of the culture medium comprises the following steps: crushing raw materials and preparing culture materials.

A recombinant camelina plant or cell comprising one or more polynucleotides encoding a Δ6-desaturase, a Δ6-elongase and a Δ5-desaturase operably linked with one or more regulatory sequences.

The invention relates to a Camelina sativa (L.) Crantz spring-type seed designated as ‘SO-50’ derived from a cross between camelina accessions with high yield and oil quality attributes following conventional breeding methodologies.

The invention relates to an agrobacterium tumefaciens-mediated camelina sativa genetic transformation method which comprises the following steps: immersing camelina sativa inflorescences during an early flowering stage into agrobacterium tumefaciens liquid of a vector plasmid carrying a target gene, performing vacuum infiltration dip-dyeing and co-culture in a dark condition, recovering normal illumination culture of camelina sativa until T0-generation seeds are harvested, sowing the T0-generation seeds to obtain T1-generation plants, performing preliminary screening on the seeds or the plants by utilizing a marker gene in the plasmid, and finally identifying a camelina sativa transgenic plant carrying the target gene by utilizing a PCR (polymerase chain reaction) amplification technology and the like. Compared with a conventional dip-dyeing method, the method is relatively high in transformation rate, successfully realizes genetic transformation of the camelina sativa, and not only lays a foundation for application of a new transformed camelina sativa transgenic variety to the fields of functional foods, biofuel and the like but also provides a method and a technical reference for gene transformation of other types of plants.

The present disclosure provides methods and compositions for modifying fatty acids in Camelina sativa oil. Fatty Acid Desaturase 2 (FAD2), Fatty Acid Desaturase 3 (FADS), and / or Fatty Acid Elongase 1 (FAE1) genes regulate fatty acid composition in camelina oil.

The invention discloses nitrilase N1 and its gene derived from Pantoea sp. ) nitrilase N3 and its gene, Leptolyngbya sp. nitrilase N4 and its gene, Brassica oleracea var. oleracea nitrilase N5 and its gene and Camelina sativa nitrilase N6 and its gene, and using the nitrilase as a biocatalyst to prepare p-aminomethylbenzoic acid intermediate p-cyanobenzoic acid. The resting cells of the corresponding nitrilase can catalyze 100g / L of the substrate, and the conversion rate is greater than 99%. The method has the remarkable characteristics of mild reaction conditions, no pollution, simple process route, etc., and has great industrial application prospects.