Marine aerobic denitrifying halomonas strain HGMN422 and application thereof

A technology of Halomonas and nitrifying salt, applied in the field of microorganisms, can solve problems such as high cost and achieve the effect of high-efficiency denitrification capacity

Inactive Publication Date: 2011-08-17
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the main disadvantage of using these processes is that it is expensive, and how to deal with the high concentration of nitrate wastewater generated by these processes itself is also a problem

Method used

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  • Marine aerobic denitrifying halomonas strain HGMN422 and application thereof
  • Marine aerobic denitrifying halomonas strain HGMN422 and application thereof
  • Marine aerobic denitrifying halomonas strain HGMN422 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The screening method of embodiment 1 marine aerobic denitrification bacterial strain:

[0018] 1. Material preparation

[0019] 1. Bacterial source and experimental wastewater

[0020] (1) Bacterial source: sediments from the seawater fish cage farm area of ​​Dapengao, Shenzhen, which has a breeding history of more than 20 years.

[0021] (2) Experimental wastewater A (high concentration of nitrate nitrogen (NO 3 - -N) Seawater): 2.6g sodium nitrate, 36g glucose, 0.5g yeast extract, 1000mL aged seawater, pH7.6-8.2, sterilized at 121°C for 20min.

[0022] (3) Experimental wastewater B (high concentration of nitrite nitrogen (NO 2 - -N) Seawater): sodium nitrite 2.1g, glucose 36g, yeast extract 0.5g, aged seawater 1000mL, pH7.6-8.2, sterilized at 121°C for 20min.

[0023] 2. Medium

[0024] (1) Selection medium: glucose 24g, sodium citrate 14.7g, potassium nitrate 6.7g, ammonium chloride 4.7g, potassium dihydrogen phosphate 1.8g, aged sea water 1000mL, sterilized a...

Embodiment 2

[0051] Example 2 Identification of marine aerobic denitrification strain HGMN422

[0052] 1. Identification of marine aerobic denitrification strain HGMN422

[0053] The marine aerobic denitrifying strain HGMN422 was identified by physiology and biochemistry and 16S rDNA molecules, and the species of the strain was determined from the molecular level, combined with the analysis of bacterial morphological characteristics and physiological and biochemical characteristics.

[0054] 16S rDNA sequence analysis mainly follows the following steps:

[0055] ① Extraction of bacterial nuclear DNA:

[0056] 1) Pick a single colony with an autoclaved toothpick and inoculate it in the expansion medium overnight, take 1.5ml of the bacterial solution into an Eppendorf tube, centrifuge at 8000rpm for 5min at room temperature, and remove the supernatant completely.

[0057] 2) Add 1.5ml of STE buffer solution to wash once, centrifuge to discard the supernatant, then add 0.6ml TE solution to ...

Embodiment 3

[0106] Example 3 Colony Morphological, Physiological and Biochemical Characteristics of Marine Aerobic Denitrifying Bacteria Strain HGMN422

[0107] The colony morphology, physiological and biochemical characteristics of the marine aerobic denitrifying strain HGMN422 are shown in Table 1-1; the cell morphology is shown in figure 1 .

[0108] Table 1-1 Colony morphology and main physiological and biochemical characteristics of marine aerobic denitrifying strain HGMN422

[0109]

[0110] Note: "+": more than 90% of the strains are positive, "-": more than 90% of the strains are negative.

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Abstract

The invention discloses a marine aerobic denitrifying halomonas strain which belongs to the Halomonas (Halomonas sp.), wherein the preservation name of the marine aerobic denitrifying halomonas is Halomonas HGMN422; the preservation company of the marine aerobic denitrifying halomonas is the China center for type culture collection; the preservation data of the marine aerobic denitrifying halomonas is on Aug 28th in 2009; and the preservation number of the marine aerobic denitrifying halomonas is CCTCC (China Center for Type Culture Collection) NO.M209186. The strain has high-efficiency denitrifying capability under the aerobic condition, can be used for rapidly and effectively removing nitrite nitrogen (NO2--N) and nitrate nitrogen (NO3--N) in sea polluted by inorganic nitrogen, has no toxic and pathogenic effects on organisms cultured in the sea and has the characteristics of safety, environment protection and high efficiency for removing pollution of high-concentration inorganic nitrogen in the mariculture environment.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and specifically relates to a marine aerobic denitrifying halomonas (Halomonas sp.) strain and its application in treating seawater polluted by inorganic nitrogen. Background technique [0002] In recent years, with the rapid development of the marine cage aquaculture industry, the problem of "self-pollution" of cage aquaculture has become increasingly prominent, which not only has a serious negative impact on the ecological environment of adjacent sea areas, but also restricts the marine aquaculture production itself. The seawater cage culture system is a high-density, high-feeding artificial breeding ecosystem. The output waste, residual bait, metabolism and excretion waste are the main sources of pollution that cause environmental problems, and finally lead to eutrophication of the seawater in the culture area. It provides a material basis for the occurrence of red tides and becomes an ...

Claims

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Application Information

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IPC IPC(8): C02F3/02C12N1/20C02F3/34
CPCY02W10/10
Inventor 黄洪辉古小莉廖秀丽黄珂孟霞
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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