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Halomonas with nitrification and denitrification effects and application thereof

A technology of denitrification and halomonas, which is applied in the field of nitrogen pollution, can solve the problems of low organic matter degradation efficiency and difficulty in reaching the standard of effluent quality, and achieve high denitrification activity and wide application prospects

Active Publication Date: 2020-11-13
THE FIRST INST OF OCEANOGRAPHY SOA +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the activated sludge in sewage treatment plants is faced with the problems of low degradation efficiency of various organic matter at low temperature and difficult to meet the quality of effluent water. Therefore, the cold-adaptive characteristics of deep-sea microorganisms are used to separate and screen out organic pollutants that can efficiently degrade organic pollutants at low temperatures. Microorganisms in extreme environments (deep sea and polar regions) and their application in environmental engineering have broad prospects

Method used

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  • Halomonas with nitrification and denitrification effects and application thereof
  • Halomonas with nitrification and denitrification effects and application thereof
  • Halomonas with nitrification and denitrification effects and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Obtaining strain DY-S025GC05

[0029] Denitrification screening medium (g / L) formula: KNO 3 0.61g / L, sodium acetate 2g / L, MgSO 4 ·7H 2 O0.05g / L, K 2 HPO 4 0.2g / L, MnSO 4 4H 2 O 0.01g / L, FeSO 4 0.01g / L, filter old sea water: ultrapure water (v / v, 2:1) to 1000ml. And after 20 minutes of high pressure damp heat sterilization at 121 ℃, set aside.

[0030] Microbial samples were collected from sediments below 3000 meters from the Ninety Degrees Ridge in the Indian Ocean. About 1 g of sediment samples were inoculated into 50 mL of denitrification screening medium, cultured in a shaker at 15 °C and 150 r / min for 7 days, and then separated Take 500 μL from the culture solution of the denitrification screening medium and transfer it to the newly prepared denitrification screening medium, culture it under the same conditions for 7 days, and repeat this step twice to enrich the denitrifying bacteria. Take the culture solution obtained from the last enrichment, and afte...

Embodiment 2

[0032] Denitrification and Nitrogen Removal by Halomonas DY-S025GC05 under Aerobic Conditions

[0033] Denitrification screening medium (g / L): KNO 3 0.61g / L, sodium acetate 2g / L, MgSO 4 ·7H 2 O0.05g / L, K 2 HPO 4 0.2g / L, MnSO 4 4H 2 O 0.01g / L, FeSO 4 0.01g / L, filter old sea water: ultrapure water (v / v, 2:1) to 1000ml. And after 20 minutes of high pressure damp heat sterilization at 121 ℃, set aside.

[0034] Halomonas DY-S025GC05 was inoculated in the denitrification screening medium, shaken at 15°C at 150rpm, and NO was detected every 24h 3- -N and NO 2 - -N concentration changes, results such as Figure 1~2 shown. The results showed that when the culture time was 24 h, Halomonas DY-S025GC05 had the strongest denitrification ability, NO 3 - -The concentration of N dropped from 40mg / L to 15mg / L, and the removal rate was 62.5%; 3 - -N removal rate can reach more than 90%; NO can be completed in 72h 3 - Complete removal of -N ( figure 1 ). DY-S025GC05 to NO ...

Embodiment 3

[0036] Nitrification and Nitrogen Removal by Halomonas DY-S025GC05 under Anaerobic Conditions

[0037] Nitrification medium (g / L): NH 4 Cl 0.19g / L, sodium acetate 5g / L, K 2 HPO 4 0.5g / L, MgSO 4 ·7H 2 O0.125g / L, MnSO 4 4H 2 O 0.01g / L, FeSO 4 0.01g / L, filter old sea water: ultrapure water (v / v, 2:1) to 1000ml. And after 20 minutes of high pressure damp heat sterilization at 121 ℃, set aside.

[0038] Halomonas DY-S025GC05 was inoculated in the nitrifying medium, filled with nitrogen and cultured in a sealed manner (15°C, 150rpm), and NH was detected every 24h 4 + -N, NO 3 - -N and NO 2 - -N concentration changes, results such as Figure 3-5 shown. The results showed that under anaerobic conditions, Halomonas DY-S025GC05, in 96h to NH 4 + The removal rate of -N reaches 83.3% ( image 3 ), at 0-96h, a certain amount of NO was accumulated in the culture solution 3 - -N( Figure 4 ) and NO 2 - -N( Figure 5 ), indicating that Halomonas DY-S025GC05 generated...

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Abstract

The invention belongs to the technical field of nitrogen pollution, and provides halomonas DY-S025GC05 with nitrification and denitrification effects and application of the halomonas DY-S025GC05. TheLatin of the halomonas DY-S025GC05 with nitrification and denitrification effects is Halomonas sp., the strain has been preserved in China General Microbiological Culture Collection Center, the address is Institute of Microbiology, Chinese Academy of Sciences, No.3, Yard 1, West Beichen Road, Chaoyang District, Beijing, the preservation date is May 19th, 2020, and the preservation number is CGMCCNO.19847. The halomonas DY-S025GC05 provided by the invention has double activities of nitrification and denitrification at the same time, and the conversion of nitrification and denitrification functions can be realized by adjusting the oxygen content.

Description

technical field [0001] The invention relates to the technical field of nitrogen pollution, in particular to a halomonas with nitrification and denitrification functions and application thereof. Background technique [0002] In recent years, due to excessive nitrogen, phosphorus and other nutrient elements in rivers, lakes and seas, the reproduction speed of algae and other aquatic organisms has been accelerated, resulting in eutrophication of water bodies, reducing the content of dissolved oxygen in water bodies, and further deteriorating the water environment. [0003] Ammonia nitrogen pollution accounts for a large proportion of water eutrophication, so people pay close attention to it. The denitrification methods in sewage treatment mainly include physical chemical method and biological method. Physical and chemical methods include breakpoint chlorination method, ion exchange method, etc. The cost of sewage treatment is high, and it has a great impact on the environment,...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/30C02F3/34C12R1/01C02F101/16
CPCC02F3/301C02F3/302C02F3/34C02F2101/16C02F2101/163C02F2101/166C12R2001/01C12N1/205
Inventor 李江
Owner THE FIRST INST OF OCEANOGRAPHY SOA
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