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Halomonas with aerobic denitrification and heterotrophic sulfur oxidation functions and application thereof

A technology of aerobic denitrification and Halomonas, which is applied in Halomonas and its application fields, can solve the problems of frequent occurrence, deterioration of breeding environment, and diseases of breeding animals, and achieve large biomass, convenient cultivation, and growth quick effect

Active Publication Date: 2021-03-26
THIRD INST OF OCEANOGRAPHY MINIST OF NATURAL RESOURCES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the intensive and high-density aquaculture of aquaculture, excessive bait is put into the aquaculture water body, resulting in a sharp deterioration of the aquaculture environment, and a large amount of ammonia nitrogen, nitrate, nitrite, etc. accumulate, causing frequent occurrence of animal diseases

Method used

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  • Halomonas with aerobic denitrification and heterotrophic sulfur oxidation functions and application thereof
  • Halomonas with aerobic denitrification and heterotrophic sulfur oxidation functions and application thereof
  • Halomonas with aerobic denitrification and heterotrophic sulfur oxidation functions and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Isolation and Identification of Halomonas sp. MCCC 1A17488 and MCCC 1A13718

[0025]1. Isolation of strains: The sulfide samples from the Tianxiu hydrothermal area in the Southwest Indian Ocean were collected, and the gradient dilution coating plate method was used to isolate and purify Halomonas sp. MCCC 1A17488 with Marine Agar solid medium. The Halomonas has been preserved in the Guangdong Microbial Culture Collection Center on March 27, 2020, and the preservation numbers are: GDMCC No: 60985. The deep-sea sediment samples in the Pacific Ocean were taken, and the gradient dilution coating plate method was used to isolate and purify Halomonas sp. MCCC 1A13718 by coating the plate with Marine Agar solid medium. The Halomonas has been preserved in the Guangdong Microbial Culture Collection Center on March 27, 2020, and the preservation numbers are: GDMCC No: 60984.

[0026] 2 Physiological and biochemical characteristics of the strain

[0027] Physiological ...

Embodiment 2

[0036] Example 2 Optimization of denitrification conditions of Halomonas sp. MCCC 1A17488 and MCCC 1A13718 in simulated wastewater

[0037] Scrape fresh bacteria from the MA plate, resuspend with sterile sea water, and adjust the bacterial concentration to 10 9 cfu / mL, according to the inoculum size of 0.2% to inoculate with 15mM NO 3 - -N or NO 2 - In the simulated wastewater of -N, the denitrification conditions of the strains were measured at different carbon sources, pH values, temperatures, salinities, and C / N ratios, and the denitrification conditions were optimized. Simulated wastewater composition: KH 2 PO 4 2g / L, Na 2 HPO 4 3g / L, MgSO 4 ·7H 2 O 0.05g / L, FeSO 4 ·7H 2 O 0.02g / L, CaCl 2 0.02g / L, distilled water 1000mL, sterilized at 121℃ for 20min. Nitrate nitrogen was determined by GB T12763 zinc cadmium reduction method; nitrite nitrogen was determined by GB7493-87-naphthaleneethylenediamine hydrochloride spectrophotometric method.

[0038] The results...

Embodiment 3

[0041] Example 3 Halomonas sp. (Halomonassp.) MCCC 1A17488 and MCCC 1A13718 Sodium Sulfide Oxidation Rate Determination

[0042] Halomonas sp. MCCC 1A17488 and MCCC 1A13718 were shaken and cultured in MB liquid medium for 24 hours, collected by centrifugation, washed twice with normal saline, and resuspended in HEPES buffer at pH 7.4 Make bacterial suspension, the final bacterial turbidity is OD 600 =2. Take 10mL of bacterial suspension, add freshly prepared sodium sulfide to start the reaction (final concentration is 1mM), and take samples at five time points of 0h, 0.5h, 1h, 1h, and 2h to determine the remaining concentration of sodium sulfide in the system. After the reaction was completed, the bacteria were collected by centrifugation again, washed twice in ultrapure water, transferred to a freeze dryer for 24 hours and freeze-dried, and the dry cell weight was measured. According to the change of sodium sulfide concentration and the dry weight of cells, calculate the ra...

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Abstract

The invention discloses halomonas with aerobic denitrification and heterotrophic sulfur oxidation functions and application thereof , and belongs to the technical field of microorganism application. The invention provides two strains of halomonas sp. MCCC 1A17488 and MCCC 1A13718 with aerobic denitrification and heterotrophic sulfur oxidation functions, and the preservation numbers of the halomonas sp. MCCC 1A17488 and the MCCC 1A13718 are as follows: GDMCC No:60985 and GDMCC No:60984. The halomonas sp. MCCC 1A17488 and MCCC 1A13718 can be applied to nitrogen and sulfur removal, can be suitable for biological nitrogen removal treatment of various nitrogen-containing wastewater, has efficient denitrification and heterotrophic sulfur oxidation capacity under aerobic and anoxic conditions, can rapidly and efficiently remove nitrate, nitrite, ammonia nitrogen, sulfide and the like, and has good application prospects in the biological nitrogen and sulfur removal of sewage, sludge, sedimentsor culture water bodies.

Description

technical field [0001] The invention belongs to the technical field of microorganism application, and in particular relates to Halomonas with functions of aerobic denitrification and heterotrophic sulfur oxidation and the application thereof. Background technique [0002] The large discharge of industrial wastewater and the long-term application of large amounts of chemical fertilizers in modern agriculture have caused nitrogen-containing compounds such as nitrate, nitrite, and ammonia nitrogen to pollute groundwater and surface water, which directly affects the quality of drinking water for people. With the intensive and high-density aquaculture of aquaculture, excessive bait is put into the aquaculture water body, resulting in a sharp deterioration of the aquaculture environment, and a large amount of ammonia nitrogen, nitrate, nitrite, etc. accumulate, causing frequent outbreaks of aquaculture animal diseases. Hydrogen sulfide is also a toxic waste gas widely present in s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/30C02F3/34C02F11/02C02F11/04C02F101/10C02F101/16
CPCC02F3/301C02F3/34C02F3/345C02F11/02C02F11/04C02F2101/163C02F2101/166C02F2101/16C02F2101/101
Inventor 邵宗泽王丽萍李建洋
Owner THIRD INST OF OCEANOGRAPHY MINIST OF NATURAL RESOURCES
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