The invention provides a biological regulation preparation. The biological regulation preparation uses polymer flooding produced water as a raw material, and after anaerobic fermentation by adding nutrients, bacteria with sulfur ion oxidizing ability, that is, sulfur oxidizing bacteria, will be formed in the fermentation liquid , sulfur-oxidizing bacteria can fix CO 2 As a carbon source, it can perform chemoautotrophic growth and can also perform chemoheterotrophic growth; sulfur oxidizing bacteria can be sulfide, elemental sulfur, sulfite, thiosulfate, thiocyanate, SDS, tetrasulfate , p-aminobenzenesulfonic acid and methionine are used as energy sources for growth metabolism. Sulfur-oxidizing bacteria have a competitive inhibitory effect on sulfate-reducing bacteria. The fermentation broth containing sulfur-oxidizing bacteria is reused for polymer flooding produced water, which can inhibit the growth of sulfate-reducing bacteria (SRB) and inhibit the microbial corrosion of substrate materials. , while maintaining the stability of polymer-containing wastewater, such water can be used to configure polymer flooding fluid to save water resources.