Enrichment culture method of sulfur bacteria
A technology of enrichment culture and sulfur bacteria, applied in the field of environmental microorganisms, can solve the problems such as failure to achieve automatic control of rapid culture and bacterial growth effects, and achieve the effect of solving substrate inhibition, salt concentration will not increase, and yield increase
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Embodiment 1
[0030] Embodiment 1 The cultivation of sulfur bacteria
[0031] The sulfur bacteria were cultivated in a 100L aerated reactor. The reactor is equipped with stirring, pH and feeding automatic control system and bacteria-water mixture discharge control system at the same time. During the cultivation process, the temperature was set at 28° C., and the pH was set at 6.8.
[0032](1) First, add 10L of microbial initial culture solution into the aeration reactor, turn on the aeration system without inoculating the microorganisms and adjust the aeration volume for aeration until the DO concentration no longer increases, thus determining the required amount of culture. The upper limit of DO concentration DOmax is 7.1 mg / L and the corresponding aeration rate, and the aeration rate is kept constant throughout the culture process. The composition and content of the microbial culture medium are: sodium sulfide 200mg / L, potassium dihydrogen phosphate 150mg / L, dipotassium hydrogen phospha...
Embodiment 2
[0036] Embodiment 2 The cultivation of sulfur bacteria
[0037] The sulfur bacteria were cultivated in a 100L aerated reactor. The reactor is equipped with stirring, pH and feeding automatic control system and bacteria-water mixture discharge control system at the same time. During the cultivation process, the temperature was set at 25°C and the pH was set at 7.0.
[0038] (1) First add 15L of microbial initial culture solution into the aeration reactor, turn on the aeration system without inoculating the microorganisms and adjust the aeration volume for aeration until the DO concentration no longer increases, thus determining the required amount of culture. The upper limit of DO concentration DOmax is 7.1 mg / L and the corresponding aeration rate, and the aeration rate is kept constant throughout the culture process. The composition and content of the microbial culture medium are: sodium sulfide 200mg / L, potassium dihydrogen phosphate 150mg / L, dipotassium hydrogen phosphate ...
Embodiment 3
[0043] The sulfur bacteria were cultivated in a 100L aerated reactor. The reactor is equipped with stirring, pH and feeding automatic control system and bacteria-water mixture discharge control system at the same time. During the cultivation process, the temperature was set at 25°C and the pH was set at 7.0.
[0044] (1) First add 20L of microbial initial culture solution into the aeration reactor, open the aeration system without inoculating the microorganisms and adjust the aeration volume for aeration until the DO concentration no longer increases, thus determining the required amount of culture. The upper limit of DO concentration DOmax is 7.1 mg / L and the corresponding aeration rate, and the aeration rate is kept constant throughout the culture process. The composition and content of the microbial culture medium are: sodium sulfide 200mg / L, potassium dihydrogen phosphate 150mg / L, dipotassium hydrogen phosphate 150mg / L, magnesium sulfate 150mg / L, ammonium chloride 30mg / L,...
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