80 results about "Achromobacter xylosoxidans" patented technology

The invention discloses an achromobacter xylosoxidans strain for biological denitrificaion and application thereof. The achromobacter xylosoxidans strain GAD3 is preserved in China General Microbiological Culture Collection Center (CGMCC for short) with preservation number CGMCC No. 2964 in March 19th, 2009. The strain is added to nitrogenous effluent with 2 to 6mg / L of dissolved oxygen to realize biological denitrificaion. The strain not only has the capability of heterotrophic nitrification, but also has the capability of aerobic denitrification. In the process of treating the nitrogenous effluent, only one aerobic stage is needed, the denitrification efficiency is high, and the operation is convenient and quick; and compared with the conventional biological denitrification process, the application has huge economic benefits.

The invention relates to a straw-decomposition composite microbial preparation and a preparation method thereof. The straw-decomposition composite microbial preparation disclosed by the invention is prepared by mixing bacillus weihenstephanensis, bacillus amyloliquefaciens, bacillus megaterium, staphylococci, achromobacter xylosoxidans and aspergillus niger according to certain mass percent, is suitable for directly returning all rice straws to the field, can outstandingly accelerate the decomposition speed of straws, is favorable to transferring the transplanting stubble, can increase the organic content, enhance the soil enzyme activity, improve the soil physicochemical property and enhance the rice yield in a next season, and can also improve the soil, enhance the water and fertilizer utilization ratio, reduce the fertilizer, increase the efficiency, reduce the pollution of straw incineration on the environment and achieve the outstanding integrated benefit.

The invention discloses a Comamonas testosteroni LH-N5 CGMCC No.6975. The invention also discloses a heterotrophic nitrification-aerobic denitrification microbial inoculum which comprises the Comamonas testosteroni LH-N5, or one or both of Achromobacter xylosoxidans subtype xylosoxidans LH-N25 and Paracoccus aminovorans LH-N40; and the strains are combined in any proportion. The invention also discloses a preparation method of the microbial inoculum. The microbial inoculum disclosed by the invention can solve the problem of difficulty in removing total nitrogen in the traditional reactor, can effectively remove ammonia nitrogen and total nitrogen in the water body in one reactor, has tolerance or degradation capability for phenols, amines, heterocycles, cyanides, polycyclic aromatic hydrocarbons and other toxic substances in wastewater, is especially applicable to nitrogenous chemical wastewater, has the advantages of simple treatment process, stable effect and environmental toxicant impact resistance, and has very wide application prospects in various actual nitrogenous chemical wastewater treatment processes.

The invention relates to a complex microbial agent for restoring coastline with heavy oil pollution, as well as a preparation method and applications thereof. The complex microbial agent comprises Sphingobacterium mizutaii, Achromobacter xylosoxidans and Achromobacter piechaudii, furthermore, the collection number of the Sphingobacterium mizutaii is CGMCC No.800; the collection number of the Achromobacter xylosoxidans is CGMCC No.8022; and the collection number of Achromobacter piechaudii is CGMCC No.8021. The complex microbial agent is good in salt resistance property, prominent in competitive advantages, and has a high-efficiency restoration effect on the crude oil pollution of coastline, especially refractory heavy oil contamination through the synergistic effect of the three bacteria, and can also be applied to restoration of saline alkali soil with overflow oil contamination.

The invention relates to application of Achromobacter xylosoxidans with a heterotrophic nitrification-aerobic denitrification and dephosphorization function in wastewater treatment. The Achromobacter xylosoxidans can metabolize by using organic carbon as a unique carbon source and using ammonia nitrogen as a unique nitrogen source and can directly convert the ammonia nitrogen into gas products through the action of heterotrophic nitrification-aerobic denitrification so as to achieve the aim of denitrification, can also take nitrate nitrogen as a unique nitrogen source and convert the nitrate nitrogen into gas products through the action of aerobic denitrification, and can further ingest inorganic phosphorus and convert the inorganic phosphorus into a tissue of the Achromobacter xylosoxidans under an aerobic condition so as to achieve the aim of removing phosphorus from wastewater. The Achromobacter xylosoxidans are applied to the wastewater treatment, the simultaneous removal of nitrogen and phosphorus can be achieved under the single aerobic condition, and the difficult problem that the biological denitrification and dephosphorization in the traditional wastewater treatment need to adopt the segmented treatments of anaerobic phosphate release, anoxic denitrification and aerobic nitrification and phosphorus absorption, is better solved, so that the Achromobacter xylosoxidans have broad application prospects.

The invention discloses complex bactericide for efficiently purifying sewage anaerobic outlet water of a pig farm and a preparation method and an application thereof. The complex bactericide is formed by mixing rhodopseudomonas palustris, bacillus subtillis, nevskia, microbacterium esteraromaticum, paracoccus and xylose oxidized achromobacter. The complex bactericide has unique purifying effect on the sewage anaerobic outlet water of the pig farm, also has a good purifying effect for artificial synthetic sewage and has a popularization application value.

The invention discloses an achromobacter strain with monomethylamine degradation ability and application thereof. The name of the strain is Achromobacter xylosoxidans GDUTAN5 which is preserved in theChina Center for Type Culture Collection at Wuhan University, No. 299, Bayi Road, Wuchang District, Wuhan, Hubei Province, China. The preservation number is CCTCC NO:M 2017285. The preservation dateis May 24, 2017. The Achromobacter xylosoxidans GDUTAN5 is Gram-negative and rod-shaped, has a round colony morphology, is light yellow and opaque, and has a smooth surface and a colony diameter of 1-2 mm. The Achromobacter xylosoxidans GDUTAN5 can be applied to environmental remediation for degradation of monomethylamine in the environment and has a high degradation rate. When the substrate concentration is 5 mg / L, the degradation rate can reach 92.3% after 96 h of degradation.

The invention discloses a bacterium 7B capable of producing cellulase separated and screened from stockpile manure. The bacterium is collected by China Center for Type Culture Collection, and the collection number is CCTCC NO:M2013365. The strain 7B is Gram-negative bacilli, and forms a smooth, wet, lustrous, offwhite bacterial colony with regular edge, of which the diameter is 0.5-1mm, on the LB (Langmuir-Blodgett) culture medium. A BIOLOG microorganism identification system and 16s rDNA sequence analysis are utilized to identify that the strain is Achromobacter xylosoxidans. A CMC (carboxymethyl cellulose) fermentation culture medium is used for culturing to obtain the 0.183U / mL carboxymethyl cellulase activity, and the Achromobacter xylosoxidans strain is hopeful to be used for cellulase production.

The invention relates to an achromobacter xylosoxidans MG1 strain and application thereof, and belongs to the field of environmental application of microorganisms. The achromobacter xylosoxidans MG1 strain is prepared by screening bacteria obtained by separating printing and dyeing wastewater by the conventional method, and was collected in China General Microbiological Culture Collection Center on December 15, 2010; and the collection number is CGMCC NO.4476. The achromobacter xylosoxidans MG1 strain has ultrastrong efficiency of decoloration on malachite green in triphenylmethane dyes, and the decolorization rate at 1 hour is up to 86+ / -1 percent when the concentration of the malachite green is up to 2,000mg / L. In addition, the MG1 strain also has a better effect of decolorizing crystalviolet, and can be applied to the preparation of biological decoloring agents. The achromobacter xylosoxidans MG1 strain has the advantages that: the range of strains which can degrade dyes can be broadened, so that an additional artificial degradation way is provided for the malachite green and the crystal violet in the environment, and the treatment cost for environmental pollution is reduced.

A novel gluconate dehydratase derived from Achromobacter xylosoxidans and a gene encoding the gluconate dehydratase are provided. By reacting the gluconate dehydratase or a transformed cell containing the gene with an aldonic acid, the corresponding 2-keto-3-deoxyaldonic acid can be efficiently produced.