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Application of arsenic oxidizing bacterium in repairing pollution of trivalent arsenic in paddy field

A technology of arsenic oxidizing bacteria and trivalent arsenic, applied in the field of environmental chemistry and environmental microorganisms, can solve the problems of secondary pollution, low efficiency, time-consuming, etc., achieve strong mobility, strong resistance and adaptability, and reduce harm Effect

Active Publication Date: 2018-05-01
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Physical methods such as adsorption, filtration, and guest-soil methods are time-consuming and costly, and are not conducive to large-scale purification; chemical precipitation and chemical oxidation / reduction are costly and easy to cause secondary pollution; phytoremediation methods (such as centipede grass) can remove soil Medium and high concentrations of arsenic pollutants are enriched in the body, but it is not suitable for the treatment of arsenic-contaminated groundwater, and the workload of planting and subsequent treatment of arsenic hyper-accumulated plants is relatively large; the microbial remediation method mainly uses microorganisms to oxidize heavy metal ions Metabolism such as / reduction, methylation / demethylation can achieve the purpose of arsenic pollution control. It is a cheap, safe and most effective repair method without causing damage to the environmental structure, which meets the needs of sustainable development. One of the most promising arsenic pollution control methods
However, this method often needs to combine the strong adsorption of pentavalent inorganic arsenic by soil or activated sludge to achieve a high removal rate, and the highly toxic trivalent arsenic in the environment is not easy to remove from the environment because it has no charge. It is urgent to efficiently oxidize trivalent arsenic to pentavalent arsenic in waste water, sewage irrigation water, and soil, but the chemical oxidation method is often not efficient enough. In practice, it is urgent to solve this technical bottleneck. High-efficiency arsenic-oxidizing bacteria are an excellent s Choice

Method used

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  • Application of arsenic oxidizing bacterium in repairing pollution of trivalent arsenic in paddy field
  • Application of arsenic oxidizing bacterium in repairing pollution of trivalent arsenic in paddy field
  • Application of arsenic oxidizing bacterium in repairing pollution of trivalent arsenic in paddy field

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Example 1: Isolation of Achromobacter xylosoxidans YYS001 from arsenic-contaminated soil

[0027] The experimental soil was taken from arsenic-contaminated soil at the foot of Zijin Mountain in Shanghang County, Fujian Province. The enrichment, isolation, purification and genus identification of arsenic-oxidizing bacteria YYS001 were carried out according to the following steps.

[0028] (1) Enrichment of arsenic-resistant bacteria: Accurately weigh 1 g of arsenic-contaminated soil into a Erlenmeyer flask filled with 150 ml of sterilized enrichment medium containing 25 mg As(III) / L, culture on a shaker at 30°C (128 rpm) for one week to observe the strain growth. When the culture medium appeared turbid, inoculate it in the enrichment medium with the next higher concentration of As(III) according to the ratio of 10% (volume ratio), and the concentrations of As(III) were 50 mg / L and 100 mg / L respectively. L, 150 mg / L, 200 mg / L, 400 mg / L, in order to achieve the purpose of...

Embodiment 2

[0034] Example 2: Arsenic oxidation curve and growth curve of Achromobacter xylosoxidans YYS001

[0035] Pick the monoclonal strain of Achromobacter xylosoxidans YYS001, inoculate it into 100mL of the most suitable liquid medium E containing 100mgAs(III) / L, and place it in a shaker at 30°C for 48 hours. Draw 5mL to 100mL of fresh optimum liquid medium E containing 150mgAs(III) / L, place it in a shaker at 30°C for 24 hours, and then draw 5mL to 100mL of fresh optimum liquid containing 200mgAs(III) / L In culture medium E, shake culture in a shaker at 30°C for 24 hours, at this time the OD of YYS001 600 It is about 0.8, stored in a refrigerator at 4°C, and used as a seed solution for inoculation. Draw 5mL from the seed bacterial solution with 5% inoculation amount into 100mL fresh optimum liquid medium E containing 1000mgAs(III) / L, put it in a shaker at 30°C for 9 hours and start sampling after 9 hours, every 1 hour Take a sample until As(III) is completely oxidized, take 1.2mL a...

Embodiment 3

[0037] Example 3: Tolerance of Achromobacter xylosoxidans YYS001 to environmental pH and temperature

[0038] Draw 5mL from the above-mentioned seed bacterial solution with an inoculum amount of 5% to 100mL fresh optimal liquid medium E containing 1000mg As(III) / L, and place it in a shaking table with different pH values ​​and different temperatures for 22 hours. After that, take a sample and measure the OD of YYS001 by spectrophotometry 600 , Take it now and do it now.

[0039] The specific method is as follows:

[0040] 1. The temperature of the fixed medium E is 35°C, placed in the medium E with a pH of 3.0~12.0, and after shaking for 22 hours, use the most suitable liquid medium E without YYS001 as the reference solution, and take each time 1.2mL, directly measure its absorbance at 600nm. The tolerance of YYS001 to the environmental pH value is shown in Table 1.

[0041] 2. The pH value of the fixed medium E is 8.5. Place it in the medium E at 25~60°C. After 22 hours o...

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Abstract

The invention provides arsenic oxidizing bacterium and application thereof in repairing pollution of trivalent arsenic in a paddy field. The arsenic oxidizing bacterium is (Achromobacter xylosoxidans)YYS001, and was preserved into Common Microorganism Center of China Committee for Culture Collection of Microorganisms on August 31th, 2017, and the collection number is CGMCC NO.14583. The arsenic oxidizing bacterium has the advantages that the toxicity of the high-toxicity trivalent arsenic in the soil of the paddy field is obviously decreased, and the better application prospect is realized inthe field of repairing of pollution of trivalent arsenic in the paddy field.

Description

technical field [0001] The invention belongs to the technical fields of environmental chemistry and environmental microorganisms, and specifically relates to an arsenic oxidizing bacterium and its application in repairing trivalent arsenic pollution in paddy fields. Background technique [0002] Arsenic pollution has become a serious environmental problem facing the world. According to reports, arsenic pollution is the most serious in Asian countries around the world, especially Bangladesh, India, and China. In my country, due to factors such as mine emissions and various arsenic-containing chemicals used in industrial and agricultural production, the arsenic content in soil, groundwater, and drinking water continues to increase, and finally passes through "soil / water → plant body / animal body → human body". The food chain is enriched and ingested, which seriously endangers human health. [0003] At present, the treatment of arsenic pollution at home and abroad mainly adopts...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/025
CPCB09C1/10C12N1/205C12R2001/025
Inventor 杨桂娣邱宗清帅培育叶惠闽王开腾杨孝军林文雄
Owner FUJIAN AGRI & FORESTRY UNIV
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