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Degradative bacteria capable of degrading allelochemicals in plant root exudates and application of degradative bacteria

A technology of allelochemicals and plant roots, applied in the field of microorganisms, can solve the problems of less research on peanut allelochemical-degrading bacteria and aggravation of peanut soil-borne diseases

Active Publication Date: 2019-09-27
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous studies have found that allelochemicals secreted by peanut roots affect the structure of root microbial communities, leading to aggravation of peanut soil-borne diseases
[0003] At present, there are few studies on peanut allelochemical-degrading bacteria

Method used

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  • Degradative bacteria capable of degrading allelochemicals in plant root exudates and application of degradative bacteria
  • Degradative bacteria capable of degrading allelochemicals in plant root exudates and application of degradative bacteria
  • Degradative bacteria capable of degrading allelochemicals in plant root exudates and application of degradative bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Screening of degrading bacteria:

[0034] The screening of degrading bacteria adopts a domestication method that uses benzoic acid as the sole carbon source and gradually increases the concentration of benzoic acid. The specific operation is as follows: accurately weigh 10 g of soil sample, add it to a triangular flask containing 90 ml of sterile water, and fully shake it in a constant temperature shaker for 30 minutes to prepare a soil suspension with a concentration of 1:10. After the soil particles settle, absorb 1ml supernatant and transfer it into a test tube containing 9ml sterile water to make 10 -2 Bacteria suspension, and so on, make 10 -3 , 10 -4 , 10 -5 , 10 -6 , 10 -7 的bacterium suspension. Choice 10 -4 , 10 -5 , 10 -6 , 10 -7 The four concentrations of 100μl each are added to the benzoic acid selection medium, and the benzoic acid concentration is 5, 10, 15, 20 mg·L -1 Increase gradient domestication and enrichment of degrading bacteria in turn. After several...

Embodiment 2

[0041] Identification of degrading bacteria:

[0042] (1) Identification of morphological characteristics of degrading bacteria

[0043] After the degrading bacteria are cultured on LB medium for 18 hours, observe the colony shape, size, color, transparency, viscosity, wetness, ridge and edge characteristics of the degrading bacteria, and whether they produce pigments, etc., and observe the bacteria with a high-power microscope after staining Gram stain, flagella, capsule, spore and other structures. After the bacterial liquid was dehydrated and freeze-dried by glutaraldehyde-fixed ethanol, the size and surface structure of the bacterial body were observed by scanning electron microscope.

[0044] (2) Determination of physiological and biochemical characteristics of degrading bacteria

[0045] After the degrading bacteria were cultured on LB medium for 18 hours, litmus milk, glucose, methyl red reaction, VP test, indole reaction, citrate, starch hydrolysis, gelatin liquefaction, cont...

Embodiment 3

[0056] Influence of environmental factors on the degradation characteristics of degrading bacteria

[0057] In order to study the degradation of benzoic acid by degrading bacteria HJ-2 and HJ-3 under different environmental conditions, three influencing factors of initial pH, temperature, and NaCl concentration were selected as the research objects, and single factor experiments were carried out.

[0058] (1) Initial culture pH

[0059] Degrading bacteria are inoculated to 20mg·L according to 0.1% (OD600nm=0.8) inoculum -1 Benzoic acid and pH 4, 5, 6, 7, 8, 9, 10, respectively, in inorganic salt culture solution, 30℃, 180r·min -1 After shaking culture for 48 hours, the growth of the bacteria and the degradation rate of benzoic acid were measured. The growth of the bacteria was compared with the culture medium without degrading bacteria, and the treatment was repeated 3 times. The absorbance value at 600nm after the treatment was measured, and the degradation rate of benzoic acid was ...

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Abstract

The invention relates to the field of microorganisms and provides degradative bacteria capable of degrading allelochemicals in plant root exudates and an application of the degradative bacteria. The degradative bacteria are classified and named Achromobacter xylosoxidans and Pseudomonas nitroreducens, and strain preservation numbers are CGMCC NO.14517 and CGMCC NO.14518 respectively. The degradative bacteria for the allelochemicals can degrade the allelochemicals in multiple degraded plant root exudates.

Description

Technical field [0001] The present invention relates to the field of microorganisms, and provides a degrading bacteria that degrades allelopathic substances in plant root exudates and applications thereof. The allelopathic substance degrading bacteria can degrade various allelopathic substances. Background technique [0002] Peanut is an important oil and economic crop in my country. Due to the limitation of soil utilization and the relatively high economic benefits of peanut, continuous peanut cropping is very common in the main peanut planting areas. However, continuous cropping of peanuts will have a continuous cropping barrier effect. The longer the continuous cropping period, the more severe the decline in peanut yield and quality, which seriously affects the sustainable development of peanut production. Autotoxicity is one of the important reasons for peanut continuous cropping obstacles. Autotoxicity refers to the inhibition of plant growth by root secretion and alleloche...

Claims

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Application Information

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IPC IPC(8): C12N1/20C09K17/14C12R1/025C12R1/38
CPCC09K17/14C09K2101/00C12N1/20C12N1/205C12R2001/38
Inventor 王小兵苏金成汪晓丽封克陈盾钱娜董君能
Owner YANGZHOU UNIV
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