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Microorganism composite bacterial agent for restoration of saline alkali soil polluted by petroleum

A compound bacterial agent, saline-alkali soil technology, applied in the restoration of polluted soil, microorganisms, microorganisms, etc., to achieve the effects of improving fertility, high removal efficiency, and improving soil physical and chemical properties

Inactive Publication Date: 2012-05-16
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the current problems in the restoration of oil-contaminated saline-alkali soil, the present invention provides a microbial composite bacterial agent suitable for repairing oil-contaminated saline-alkali soil, which can quickly and effectively restore oil-contaminated saline-alkali soil

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: Inject beef extract peptone medium (component ratio is : Beef extract 5g, peptone 10g, NaCl 5g, water 1000ml, pH7.0-7.2, sterilized at 121°C for 20 minutes), cultured on a constant temperature shaker at 37°C at 150r / min for 48 hours. Centrifuge at 5000r / min for 10 minutes, remove the bacterial cell pellet in the lower layer, and wash twice with phosphate buffer solution (concentration: 0.02-0.1 mol / l) of pH 7.0-7.5. The obtained cell pellets were mixed in a ratio of 1 to 3:1 to 3:1 to 3 by weight, and resuspended in pH 7.0 to 7.5 phosphate buffer (concentration of 0.02 to 0.1 mol / l), the composite microbial bacterial liquid can be obtained. Mix the compound microbial bacterial liquid, ammonium sulfate, potassium dihydrogen phosphate, Triton-X100 and sodium dodecylsulfonate in a ratio of 100:1.5:1:0.3:0.3 to obtain the oil-polluted saline-alkali restoration described in the present invention Soil microbial complex agent.

Embodiment 2

[0017] Embodiment 2: Add the prepared microbial composite bacterial agent into oil-contaminated saline-alkali soil at an application rate of 1%, and mix evenly. After 10 days, the soil petroleum hydrocarbon content was measured. The content of alkanes decreased from 3870mg / kg to 2748mg / kg, a decrease of 29%; the content of aromatics decreased from 793mg / g to 605mg / kg, a decrease of 24%. Then add 1% microbial compound bacteria agent to the oil-contaminated saline-alkali soil, and mix evenly. After 10 days, the alkane content dropped to 2060 mg / kg, and the cumulative reduction was 47% in 20 days; the aromatic hydrocarbon content dropped to 507 mg / kg, and the cumulative reduction was 36%.

Embodiment 3

[0018] Embodiment 3: Add the prepared microbial composite bacterial agent into oil-contaminated saline-alkali soil at an application rate of 2%, and mix well. After 7 days, the physical and chemical properties of the soil were measured. The content of alkanes decreased from 3870mg / kg to 2718mg / kg, a decrease of 30%; the content of aromatic hydrocarbons decreased from 793mg / g to 603mg / kg, a decrease of 24%. Then add 2% microbial composite bacteria agent to the oil-contaminated saline-alkali soil and mix evenly. After 10 days, the alkane content dropped to 1823 mg / kg, and the cumulative reduction was 53% in 20 days; the aromatic hydrocarbon content dropped to 430 mg / kg, and the cumulative reduction was 46%.

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Abstract

The invention relates to a microorganism composite bacterial agent applicable to the restoration of saline alkali soil polluted by petroleum, and a preparation method thereof, and belongs to the technical field of environment protection. The bacterial agent mainly comprises the following 3 components: composite microorganism bacterial liquid, nutrients, and a surfactant. The composite microorganism bacterial liquid is obtained by performing liquid culture of 3 petroleum hydrocarbon degrading bacteria of bacillus megaterium P9 strains (with a preservation number of CGMCC No. 4270), pseudomonas sp. P4 strains (with a preservation number of CGMCC No. 4269), and achromobacter xylosoxidans P2 strains (with a preservation number of CGMCC No. 4268) to obtain microorganism bacterial strains, and mixing the strains with the nutrients. The bacterial strains are preserved at China microorganism strain preservation management committee general microbiological center. The composite microorganism bacterial liquid, the nutrients and the surfactant are mixed according to a ratio of 30-200:1-2:0.2-0.5 so as to obtain the microorganism composite bacterial agent. The microorganism composite bacterial agent prepared by the invention has high removing efficiency for petroleum pollution in saline alkali soil, can both reduce the pH value of the saline alkali soil, and improve soil physical and chemical properties.

Description

Technical field: [0001] The invention belongs to the technical field of environmental protection, and relates to a microbial composite bacterial agent suitable for remediation of oil-polluted saline-alkali soil and a preparation method thereof. Background technique: [0002] Due to the specific geological conditions and unreasonable human activities, the soils of Daqing Oilfield, Dagang Oilfield, Shengli Oilfield and other oilfields in my country have different degrees of salinization. In the process of oil exploitation and oil well construction, it is easy to cause leakage of petroleum hydrocarbons and pollute the soil. The physical and chemical properties of saline-alkali soil itself are poor, and oil pollution further deteriorates the physical and chemical properties of saline-alkali soil, causing significant stress to soil microorganisms and interfering with soil material circulation. In addition, pollutants such as polycyclic aromatic hydrocarbons contained in petroleu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/20B09C1/10C12R1/11C12R1/38C12R1/025
Inventor 庄国强王新新朱永官韩祯姜丹白志辉
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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