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Complex bactericide for efficiently purifying sewage anaerobic outlet water of pig farm and preparation method and application thereof

A technology of anaerobic effluent and compound bacterial agents, which is applied in the direction of biochemical equipment and methods, chemical instruments and methods, and methods based on microorganisms, can solve environmental hazards and other problems, achieve reduced suspended solids, unique purification effects, and increase synergies The effect of symbiosis

Active Publication Date: 2015-12-23
FUJIAN INST OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If a large amount of high-concentration anaerobic effluent without subsequent treatment is directly discharged, it will inevitably cause great harm to the environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1: Preparation of composite bacterial agent for efficiently purifying pig farm sewage anaerobic effluent

[0014] (1) Incline cultivation:

[0015] Rhodopseudomonas palustris FIM-20150806001 was inoculated on slant medium 1, and cultured for 48-72 hours at 30°C and 2500Lx light.

[0016] Slant medium 1 formula (g / L): sodium dihydrogen phosphate 5.5, malic acid 1.5, sodium acetate 2.0, sodium hydroxide 2.0, ammonium chloride 1.0, magnesium chloride 0.25, calcium chloride 0.05, yeast extract 1.0 , agar 15.0, trace elements 1mL, pH 7.0, the balance is water, mix the above ingredients evenly, and sterilize for later use. Trace element formula (mg / L): ferric trichloride hexahydrate 5.0, zinc sulfate heptahydrate 1.0, copper sulfate pentahydrate 0.05, cobalt nitrate hexahydrate 0.5, boric acid 1.0, manganese chloride tetrahydrate 0.05, and the balance is water.

[0017] Inoculate Bacillus subtilis FIM-20150806002, Nevaella FIM-20150806003, Microbacterium acetoflavin...

Embodiment 2

[0028] Example 2 The domestication test of the complex bacterial agent for efficiently purifying the anaerobic effluent of pig farm sewage in the present invention to the anaerobic effluent of pig farm sewage.

[0029] Domestication and cultivation of the anaerobic effluent of pig farm sewage for the composite bacterial agent (composite bacterial agent for efficient purification of anaerobic effluent of pig farm sewage anaerobic effluent) of efficient purification of pig farm sewage anaerobic effluent: Take the high-efficiency purification of pig farm sewage in Example 1 The composite bacterial agent-1 of anaerobic effluent is inoculated into 1 / 5 anaerobic effluent+4 / 5 medium with an inoculation amount of 5% by volume (the medium is composed of 1 / 2 volume of fermentation medium 1 and 1 / 2 volume of fermentation medium 2) for acclimatization, wait for it to grow to the logarithmic phase, then inoculate it into 2 / 5 anaerobic effluent + 3 / 5 medium for acclimatization, wait for it t...

Embodiment 3

[0031] Example 3 The purification test of synthetic sewage by the composite bacterial agent for efficiently purifying pig farm sewage anaerobic effluent in the present invention

[0032] Preparation of synthetic sewage, composition (mg / L): glucose 360, yeast extract 80.0, KH 2 PO 4 14.0, MgSO 4 ·7H 2 O24.0, NH 4 Cl60.0, CaCl 2 18.0, NaHCO 3 24.0, MnSO 4 ·7H 2 O6.0, FeCl 3 0.3, NaNO 2 30.0, KNO 3 30.0, dissolved in 1L of distilled water, and adjusted to pH 7.0. Packed in 500mL Erlenmeyer flasks. Autoclave at 121°C for 15 minutes.

[0033] According to the composite bacterial agent for efficiently purifying pig farm sewage anaerobic effluent: the volume ratio of synthetic sewage=1:10000 is inserted in the synthetic sewage into the composite bacterial agent for efficiently purifying pig farm sewage anaerobic effluent (efficiently purifying pig farm sewage) Composite bacterial agent-1) of sewage anaerobic effluent, respectively insert the Rhodopseudomonas palustris ba...

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PUM

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Abstract

The invention discloses complex bactericide for efficiently purifying sewage anaerobic outlet water of a pig farm and a preparation method and an application thereof. The complex bactericide is formed by mixing rhodopseudomonas palustris, bacillus subtillis, nevskia, microbacterium esteraromaticum, paracoccus and xylose oxidized achromobacter. The complex bactericide has unique purifying effect on the sewage anaerobic outlet water of the pig farm, also has a good purifying effect for artificial synthetic sewage and has a popularization application value.

Description

Technical field: [0001] The invention belongs to the field of sewage treatment, and in particular relates to a composite bacterial agent for efficiently purifying pig farm sewage anaerobic effluent, a preparation method and application thereof. Background technique: [0002] With the rapid development of intensive and large-scale pig farming in my country, the excrement and sewage discharged from large-scale pig farms is also increasing dramatically. Many pig farms adopt the process of flushing manure with water, which takes concentrated time for pen flushing, a large amount of waste water, and a lot of feces, high concentration of pollutants, and difficult treatment. The sewage treatment modes of aquaculture industry in various countries in the world can be summarized into two types: one is the returning mode with the goal of resource utilization, and the other is the artificial enhanced treatment mode with the goal of meeting the discharge standards. Large-scale pig farms...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/125C12R1/01C02F103/20
Inventor 聂毅磊陈宏贾纬罗立津
Owner FUJIAN INST OF MICROBIOLOGY
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