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Achromobacter xylosoxidans strain and application thereof

A colorless bacillus, xylose technology, applied in bacteria, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of increased BOD, unsatisfactory discharge, and few reports on microbial degradation methods.

Inactive Publication Date: 2010-12-15
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Application of laccase to decolorize printing and dyeing wastewater containing indigo, BOD increased significantly after decolorization, COD remained basically unchanged, still high, and could not be discharged up to the standard
The decolorized product contains indole quinone and anthranilic acid, and the application of laccase to decolorize indigo is still in the research stage, and there are few reports on the microbial degradation of indole quinone and anthranilic acid after decolorization

Method used

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  • Achromobacter xylosoxidans strain and application thereof
  • Achromobacter xylosoxidans strain and application thereof
  • Achromobacter xylosoxidans strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1. Isolation, purification and identification of Achromobacter xylosoxidans N4 CGMCC No. 3632.

[0020] In May 2009, the activated sludge from the denim printing and dyeing wastewater treatment tank (collected from a denim printing and dyeing factory in Haining City, Zhejiang Province) was diluted with sterile water, and coated with solid medium (1L solid medium contains 3g of beef extract, Peptone 10g, NaCl 5g, agar 15g, and the rest are water) plate, 18 bacterial strains were isolated, and the bacterial strain was purified by streaking on the plate. The isolated and purified 18 strains were respectively inserted into liquid medium (1L medium contains 3g of beef extract, 10g of peptone, 5g of NaCl, and the rest is water) with an inoculation loop for liquid culture. The culture temperature was 28°C and 180rpm for 24- After 48h, join in indoloquinone or anthranilic acid aqueous solution with 5% (volume percentage content) inoculum, liquid chromatography measureme...

Embodiment 2

[0025] Example 2. Validation test of the effect of Achromobacter xylosoxidans N4 CGMCC №.3632 on the degradation of indoloquinone

[0026] 1. Verification test on the degradation effect of 100 mg / L indoloquinone

[0027] Medium for Achromobacter xylosoxidans N4 CGMCC №.3632:

[0028] Solid medium (1L): 3g of beef extract, 10g of peptone, 5g of NaCl, 15g of agar, and the rest is water.

[0029] Liquid medium (1L): 3g of beef extract, 10g of peptone, 5g of NaCl, and the rest is water.

[0030] Inoculate Achromobacter xylosoxidans (Achromobacter xylosoxidans) N4 CGMCC №.3632 into the liquid medium, and cultivate it at 28°C and 180 rpm for 24-48h until the OD of the culture medium is reached. 600 0.6, then in a non-sterile environment, the culture solution was inoculated into the aqueous solution of 100 mg / L indoloquinone according to the volume percentage of 5%, and the control group was added with 5% distilled water according to the volume percentage, at 28 ° C, Cultivate at ...

Embodiment 3

[0037] Example 3, Achromobacter xylosoxidans (Achromobacter xylosoxidans) N4 CGMCC №.3632 effect verification test on the degradation of the mixture of indoloquinone and anthranilic acid

[0038] Medium for Achromobacter xylosoxidans N4 CGMCC №.3632:

[0039] Solid medium (1L): 3g of beef extract, 10g of peptone, 5g of NaCl, 15g of agar, and the rest is water.

[0040] Liquid medium (1L): 3g of beef extract, 10g of peptone, 5g of NaCl, and the rest is water.

[0041] Inoculate Achromobacter xylosoxidans (Achromobacter xylosoxidans) N4 CGMCC №.3632 into the liquid medium, and cultivate it at 28°C and 180rpm for 24-48h until the OD of the culture medium is reached. 600 0.6, then in a non-sterile environment, the fermentation broth was inoculated into a mixed system containing 100mg / L indoloquinone and 100mg / L anthranilic acid in an amount of 5% by volume, and the control group was in the mixed system Adding distilled water with a volume percentage of 5%, cultivated at 28°C and...

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Abstract

The invention discloses an Achromobacter xylosoxidans strain and application thereof. The strain is Achromobacter xylosoxidans N4 with the collection number of CGMCC No.3632. The Achromobacter xylosoxidans N4 GCMCC No.3632 can quickly and efficiently degrade isatin and o-aminobenzoic acid, and can be used for biologically treating calico printing wastewater.

Description

technical field [0001] The invention relates to a strain of Achromobacter xylosoxidans and application thereof. Background technique [0002] Denim printing and dyeing wastewater contains a large amount of indigo, resulting in high chroma, and the biodegradability of indigo is poor, so it is difficult to directly treat the printing and dyeing wastewater containing indigo with microorganisms. The use of laccase to decolorize indigo to treat printing and dyeing wastewater has the advantages of no secondary pollution, reusability, and low cost compared with the current physical photooxidation method or the method of depositing indigo with chemical reagents, and the current denim printing and dyeing factory Wastewater contains not only indigo, but also a large amount of sulfur black dye. If laccase is used to decolorize indigo, sulfur black can be easily precipitated and recovered. Otherwise, the two dyes can be precipitated together by precipitating printing and dyeing wastewat...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/025C02F101/38C02F103/30
Inventor 王雁萍董湘熔秦广雍谈重芳李宗伟常胜和李月艳
Owner ZHENGZHOU UNIV
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