The invention discloses a preparation method of mono-sulfonated Lewis
trisaccharide. The preparation method of the mono-sulfonated Lewis
trisaccharide comprises the following steps: (1) dissolving 300-350 mg of 8-p-methoxy-benzeneoctanoxy 2-acetamido 3-O-(b-D-galactopyranoside) 4-O-(2, 3, 4-tri-O-benzyl a-L-fucopyranoside) 2-deoxidation-b-D-glucopyranoside 0.3 mmol / L in 3-5 mL of
anhydrous pyridine, then adding 85-100 mg of a
sulfur trioxide pyridine complex 0.6 mmol / L, mixing and stirring for 14-15.5 hours at the temperature of 78-82 DEG C, adding methylbenzene, and evaporating a cosolvent to obtain original residues; (2) dissolving the original residues in 4-5 mL of a mixed
solvent of
methanol and acid, adding a
palladium or carbon catalyst, stirring for 25-32 hours at the
room temperature under the condition that
hydrogen exists, and removing the catalyst and the mixed
solvent to obtain residues; (3) placing the residues in DEAE-
Sephadex A-50 columns, removing sulfated saccharide by
elution with water, and eluting a mono-sulfonatedsaccharide component and a sulfated saccharide component by using a NaCl solution with the
molar concentration of 5% and a NaCl solution with the
molar concentration of 15% separately; and (4) loading residues obtained after a mono-sulfonated fraction is desolventized on C-18 columns, eluting the residues with water and then washing the residues with
methanol to obtain the mono-sulfonated Lewis
trisaccharide. The preparation method is the most
effective method for generating
sulfonic acid oligosaccharideuntil now.