The invention discloses a
porphyridium culture medium formula and a
plastic film bag semi-continuous culture method, and relates to the field of ocean microalgae culture. The
porphyridium culture traditionally adopts an SBM
nutrient solution formula, the growth rate is slow, and the yield is low. The experiments of the inventor show that NH4NO3 is taken as a
nitrogen source, an appropriate amount of NaHCO3 is added, and
gibberellin, A8 trace elements and f / 2
vitamin are supplemented, so that the
nutrition is more balanced and complete, the
porphyridium growth rate is greatly increased, and the yield can be improved by 78%. The porphyridium culture mode is that the inventor adopts the
plastic film bag semi-continuous culture method to replace the conventional
open type cement tank batch culture, so microalgae cells are kept with appropriate nutritive salt concentration during the growth process all the time, and the
plastic film bag semi-continuous culture method has the characteristics of easiness for operation, difficulty for
pollution, easiness for mastering, flexibility, diversity, difficulty for
scratching, safety, reliability, good
light transmission, low cost, quick growing and high yield.