Porphyridium culture medium formula and plastic film bag semi-continuous culture method

Abstract

The invention discloses a porphyridium culture medium formula and a plastic film bag semi-continuous culture method, and relates to the field of ocean microalgae culture. The porphyridium culture traditionally adopts an SBM nutrient solution formula, the growth rate is slow, and the yield is low. The experiments of the inventor show that NH4NO3 is taken as a nitrogen source, an appropriate amount of NaHCO3 is added, and gibberellin, A8 trace elements and f / 2 vitamin are supplemented, so that the nutrition is more balanced and complete, the porphyridium growth rate is greatly increased, and the yield can be improved by 78%. The porphyridium culture mode is that the inventor adopts the plastic film bag semi-continuous culture method to replace the conventional open type cement tank batch culture, so microalgae cells are kept with appropriate nutritive salt concentration during the growth process all the time, and the plastic film bag semi-continuous culture method has the characteristics of easiness for operation, difficulty for pollution, easiness for mastering, flexibility, diversity, difficulty for scratching, safety, reliability, good light transmission, low cost, quick growing and high yield.

Description

technical field [0001] The invention belongs to the field of marine microalgae cultivation, and in particular relates to a culture medium formula of Porphyridum and a method for semi-continuous cultivation of plastic film bags. Background technique [0002] Porphyridium cruentum belongs to Rhodophyta, Protoerythrophyta, Porphyrides, Porphyridaceae, and Porphyridium genus. It is a marine single-celled microalgae that presents purple or deep red in different growth stages. It contains Rich in polyunsaturated fatty acid PUFAs such as arachidonic acid AA and eicosapentaenoic acid EPA. AA and EPA are important essential fatty acids in the human body. AA has the functions of regulating cardiac excitability, participating in neuroendocrine, promoting cell division and inhibiting platelet aggregation. The precursor of triene can improve blood lipid quality, reduce cholesterol, blood viscosity and blood sugar concentration, and can effectively treat and prevent coronary heart diseas...

AI Technical Summary

Problems solved by technology

The fed-batch culture and continuous culture mode can effectively adjust the nutrient concentration in the growth process of algae cells, so that the nutrient concentration in the culture solution can be maintained at an appropriate level, which can not only reduce the inhibition and toxicity caused by the higher initial nutrient concentration, but also make the algal The delay period of somatic growth is greatly shortened, and it can effectively solve the problem of nutrient limitation in batch culture, ensure the continuous supply of nutrient salts, keep the microalgae in the logarithmic growth phase for a long time, and improve the proliferation efficiency, but the operation is troublesome in practical applications. The equipment is more complicated and the cost is high

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