Method for extracting phycoerythrin with assistance of spray freeze dryer

Abstract

The invention devises a method for extracting phycoerythrin by drying a porphyridium solution with assistance of a spray freeze dryer. The method for extracting the phycoerythrin by drying the porphyridium solution with assistance of the spray freeze dryer comprises the following steps: drying a porphyridium solution by using a spray freeze dryer so as to rapidly and effectively obtain dried porphyridium powder; mixing the dried porphyridium powder with water at certain mass-volume ratio, and obtaining a crude phycoerythrin extract by adopting a freeze-thaw method; performing one-step purification on the crude phycoerythrin extract so as to obtain a high-concentration high-purity phycoerythrin solution; and then, preserving the high-concentration high-purity phycoerythrin solution in a refrigerator at 4 DEG C or directly using the high-concentration high-purity phycoerythrin solution. By preparing the dried porphyridium powder by adopting a vacuum spray freeze drying way, the dried porphyridium powder prepared by the method is uniform in particle size, as well as fine and smooth in texture; and thus, phycoerythrin extraction is facilitated. In addition, the phycoerythrin is purified by adopting an one-step purification way, so that, the method is simple in process, and capable of achieving a purified phycoerythrin solution concentration up to 0.9 g / L or above as well as a purity PI value up to 2.2 or above; and thus, requirements on food-grade additives are met. Therefore, the phycoerythrin can be widely applied in the food industry, and is worthy of further studying.

Description

Technical field [0001] The invention relates to the technical field of marine microalgae, in particular to a method for auxiliary extraction of phycoerythrin by a spray freeze dryer. Background technique [0002] Porphyridium vulgaris has the characteristics of high biomass production and rich high value-added products among many microalgae strains, because porphyra vulgaris cells can synthesize a variety of biologically active substances during the growth process, such as: phycoerythrin PE, extracellular Sulfated lipopolysaccharides, arachidonic acid ARA and eicosapentaenoic acid EPA have broad application prospects in medicine, cosmetics, food, health products, textile, printing and dyeing, petroleum and other industries. [0003] In recent years, as natural products have received more and more attention from people, the value of phycoerythrin has been gradually discovered. Phycoerythrin is a natural and excellent water-soluble pigment protein that serves as the main light supple...

AI Technical Summary

Problems solved by technology

The current dehydration methods for producing Porphyridium algae powder include flocculation precipitation method, centrifugal separation method, natural sedimentation method, membrane filtration method, etc. Wrapped with a thick mucous membrane, the viscosity is too high and it is not easy to settle, which brings a huge challenge to dehydration. The flocculation sedimentation method, natural sedimentation method, and membrane filtration method are often difficult due to the high biomass concentration and viscosity of the algae liquid. Problems such as sedimentation, long production cycle, clogged filter membrane, etc.; while the centrifugation method requires high equipment, the output of one centrifugation is not high, and multiple complicated centrifugations are required, which brings a greater burden to production; currently, phycoerythrin The extraction and purification methods are diversified, such as: liquid nitrogen grinding extraction method, ultrasonic extraction method, bead mill crushing extraction method, freeze-thaw crushing extraction method and salting out purification method, ultrafiltration purification method, column chromatography purification method, etc., according to Depending on the production requirements, an appropriate extraction and purification method is usually selected to better meet the development requirements of high-value energy utilization

However, the liquid nitrogen grinding method, ultrasonic crushing method, and bead milling method are difficult to realize on a large scale in industry, and the ultrafiltration purification method and column chromatography purification method have problems such as high equipment requirements

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