36 results about "Porphyridium sp." patented technology

This invention focuses on a novel process in which Porphyridium cruentum biomass first undergoes a stage of cellular disruption and subsequently stages of recovery and purification in order to achieve the purified B-phycoerythin (BFE) protein dye, using isoelectric precipitation and two-aqueous-phase systems. The steps of recovery and purification include isoelectric precipitation followed by a step of liquid / liquid extraction by means of two-aqueous-phase systems that use polyethylene glycol (PEG) and phosphate salts. The BFE protein dye obtained in the two-aqueous-phase extraction step undergoes an ultrafiltration step in order to remove the polymer (PEG) and to obtain a dye with a purity greater than 4.0 defined as the relationship between the absorbencies at 545 and 280 nm (BFE purity=Abs 545 nm / Abs 280 nm).

The invention relates to the technical field of cosmetics and in particular relates to a long-lasting moistening skin conditioning agent and a preparation method and application thereof. The skin conditioning agent is prepared by extracting the following raw materials in percentage by weight: 5-30% of porphyridium, 5-30% of Skeletonema costatum, 5-30% of tremella fuciformis, 3-30% of lentinus edodes, 5-30% of dahurian larch, and 5-30% of ophiopogon root. The conditioning agent has excellent moistening effect, can be used for effectively reducing dandruff, dryness heat and other symptoms caused by skin dryness; in addition, the conditioning agent has good affinity with skin membrane protein, is capable of enhancing the absorption of skin to the nutrients, increasing the hydration function of the cells, and ensuring that the self tolerance of the skin in a dry environment is improved.

The invention belongs to the technical field of processing of health-care products and relates to a compound mulberry preparation capable of improving superoxide dismutase activity. The preparation method for the compound mulberry preparation comprises the following steps of: extracting 12g of mulberry and 9g of medlar in ethanol of which the concentration is 70 percent and the amount is 10 timesthat of the mulberry and the medlar at room temperature for 10 hours, sealing and crushing the extract with ultrasonic waves for 30 minutes, filtering the extract, and extracting the filtration residue with ethanol of which the concentration is 70 percent and the amount is 6 times and 4 times that of the filtration residue again for one time respectively; combining the filtrates, reclaiming the ethanol to obtain an extract of the mulberry and medlar; centrifuging 20g of porphyridium cruentum with a centrifugal for 10 minutes, suspending the porphyridium cruentum in 50mmol / L of phosphate buffer of which the pH value is 7.4, crushing the porphyridium cruentum with ultrasonic waves, centrifuging the crushed porphyridium cruentum with a high-speed centrifugal for 15 minutes to obtain a liquidsupernatant, and refrigerating and drying the liquid supernatant to obtain a dried product; and uniformly mixing the dried product and the extract of the mulberry and medlar to obtain the mulberry preparation. The mulberry preparation can be applied to a natural antioxidant which is newly developed in fields, such as food, medicament and the like, and contributes to industrial production.

The invention discloses a composition for rosacea, and can achieve the effects of balancing micro-ecology of skins, reducing sensitivity of the skins, alleviating skin inflammation, improving red pimples of faces and recovering skin barriers. The invention also discloses a compound preparation of the rosacea, and can realize effective cosolvency of 4-terpilenol, a radix salviae miltiorvhizae extract, a syringa vulgaris extract, a porphyridium extract, ceramide and cholesterin in the compound preparation, so that the common action can achieve the effects of balancing the micro-ecology of skins,reducing the sensitivity of the skins, alleviating the skin inflammation, improving the red pimples of faces and recovering the skin barriers; and meanwhile, the preparation is good in stability whenbeing stored under a room temperature condition. The invention also provides a preparation method of the compound preparation for rosacea.

The invention discloses a plant formula with lifting, firming and anti-wrinkle effects and application thereof. The plant formula is mainly prepared from the following raw materials in parts by mass:1-10 parts of longan seeds, 1-10 parts of porphyridium and 1-10 parts of pomegranate flowers. The botanical formula has the effects of inhibiting cutis laxa and increasing wrinkles, and has the characteristic of good safety. When the plant formula is used in cosmetics, the effect is better than existing similar products.

The invention discloses a high-density and high-yield porphyridium continuous culture method. The method comprises the following steps: step 1, culturing porphyridium in a photobioreactor at high density to the middle and later periods of a logarithmic phase; step 2, continuously harvesting algae cell parts in high-density algae liquid; step 3, harvesting soluble exopolysaccharides in a supernate obtained by continuous harvesting, and supplementing the harvested supernate back to the photobioreactor; step 4, controlling the speed of feeding a culture medium concentrated solution into the photobioreactor; and repeating the steps 2-4 until the culture is ended. The method has the characteristics of high density, high yield and continuous production, can simultaneously harvest purple ball biomass, phycoerythrin, exopolysaccharide, polyunsaturated fatty acid and the like at high yield, realizes reuse of the culture solution, and improves the economical efficiency and environmental protection property of culture.

The invention belongs to the technical field of immunoregulation, and particularly relates to an algal polysaccharide composition and a preparation method and application thereof. The algal polysaccharide composition is prepared from, by mass, 22-26% of spirulina polysaccharide, 36-40% of ulva polysaccharide, 12-16% of laminarin, 14-16% of spirulina polysaccharide and 6-12% of porphyridium polysaccharide. The algal polysaccharide composition and the preparation method thereof provide an immunoregulation product which is free of toxic and side effects, high in quality and low in price.

The present disclosure relates to compositions containing a natural extract(s) and their fraction(s) and the use of such compositions for treatment of skin, hair and nail. For example, the present disclosure relates to compositions containing an aqueous extract of (i) Yacon leave, (ii) Amor Seco leave, or (iii) Porphyridium biomass, or a combination thereof and a pharmaceutically or cosmetically acceptable carrier use on hair skin and nails for cosmetic purposes.

The invention discloses multiple-effect activation eye activating concentrate and a preparation method thereof. The multiple-effect activation eye activating concentrate comprises the following raw materials of a yeast fermentation product extract, acetyl hexapeptide-8, acetyl tetrapeptide-2, acetylated sodium hyaluronate, phoenix dactylifera seeds, a porphyridium cruentum extract, an emulsifyingagent, an emollient, a thickening agent and a preservative. The multiple-effect activation eye activating concentrate prepared by the preparation method disclosed by the invention has relatively strong antioxygenic property, can remove redundant free radicals in a body, activate skin cells, promote metabolism of the skin cells, reduce loss of collagen and elastin, improve black eyes, reduce eye wrinkles, improve eye skin compactness, improve skin roughness and resist aging, and is durable and stable in efficacy, and mild and non-irritant in action.

The invention discloses a composition containing porphyridium polypeptide and a preparation method of the composition. The composition comprises the following effective components in parts by weight:0.1-0.9 part of porphyridium polypeptide and 99.1 to 99.9 parts of a substrate. The preparation method comprises the following steps: an obtaining step of the porphyridium polypeptide: repeatedly freezing and thawing porphyridium, performing ultrasonic crushing, centrifuging an obtained porphyridium liquid, taking supernate, performing separating by vertical electrophoresis to obtain polypeptide with molecular weight of 5Kda or below, namely the porphyridium polypeptide; or taking the porphyridium, performing homogenizing, sterilizing, filtering and centrifuging, collecting supernatant, and performing ultrafiltration by using an ultrafiltration membrane system with a microporous membrane of 0.001-0.1 [mu]m to obtain the porphyridium polypeptide with the molecular weight cutoff 1KDa; and amixing step: mixing the porphyridium polypeptide with the matrix in parts by weight to obtain the composition containing the porphyridium polypeptide. The composition can promote wound healing, reducewound opening time and facilitate rehabilitation after body surface damage.

The invention discloses a method for simultaneously extracting multiple active substances in porphyridium, and belongs to the field of bioengineering. The method comprises the following steps: firstly, leaching porphyridium with cold water, separating a cold water leaching solution and porphyridium residue I, leaching the porphyridium residue I with ethanol or an ethanol aqueous solution, separating an ethanol or ethanol aqueous solution leaching solution and porphyridium residue II, leaching the porphyridium residue II with hot water, and separating a hot water leaching solution and porphyridium residue III, wherein the cold water leaching solution is a phycoerythrin extract product, the ethanol or ethanol aqueous solution leaching solution is a polyunsaturated fatty acid extract, and the hot water leaching solution is an intracellular polysaccharide extract. The extraction method is high in extraction efficiency, safe, simple to operate, non-toxic and beneficial to large-scale popularization and subsequent product development.

The invention discloses an alga-containing composition used for a cosmetic. The composition in dry weight contains the following components in percentage by mass: 0.003-0.125% of an alga mixture, 0.001-0.050% of radix ophiopogonis extract, 0.100-25.000% of betaine and the balance of deionized water, wherein the alga mixture is composed of porphyridium cruentum extract, bladderwrack extract and chondrus crispus extract, the content of the porphyridium cruentum extract is 0.001-0.005%, the content of the bladderwrack extract is 0.001-0.030%, and the content of the chondrus crispus extract is 0.001-0.090%. The invention also discloses a cosmetic composition applying the composition used for the cosmetic. The alga-containing composition used for the cosmetic has the effects of nature, high efficiency, easiness in absorption by virtue of a human body and long moisture preserving time and is applicable to manufacturing of various moisture preserving type skincare products.

The invention relates to the technical field of gene engineering, and particularly relates to a porphyridium chloroplast homologous recombination empty vector and application thereof. The vector comprises an upstream homologous arm, a downstream homologous arm, promoters and terminators, wherein a base sequence of the upstream homologous arm is shown in SEQ ID NO:1 of a porphyridium chloroplast gene group, a base sequence of the downstream homologous arm is shown in SEQ ID NO:2, base sequences of the promoters are shown in SEQ ID NO:3 and SEQ ID NO:4, base sequences of the terminators are shown in SEQ ID NO:5 and SEQ ID NO:6; and a corresponding base sequence which can form a multicistron structure with at least one exogenous gene and is shown in SEQ ID NO:7 is inserted between the corresponding promoter and the corresponding terminator. By adopting the porphyridium chloroplast homologous recombination empty vector disclosed by the invention, stable expression of multiple exogenous genes in the chloroplast can be realized.

The invention discloses an application of a compound 3,3',5,5'-tetrabromo-2,2'-biphenol in the aspect of algal inhibition. Algae removal spectrum test proves that the compound has excellent inhibitioneffect on eleven algae comprising thalassiosira pseudonana, chaetoceros mulleri, prorocentrum minimum, amphidinium carterae, nannochloropsis oculata, prochlorococcus, dunaliella salina, phaeodactylumtricornutum, chlorella vulgaris, tetraselmis subcordiformis, porphyridium cruentum, etc. The compound has great significance on treatment of red tide, algal bloom and the like natural hazard and canprevent eutrophication of water body.

The invention belongs to the technical field of alga culture and discloses a technology for producing purified phycoerythrobilin. The technology comprises the following steps: step 1) preparing a porphyridium cruentum seed solution; step 2) carrying out large-scale culture; and step 3) separating and purifying. By adopting the technology provided by the invention, the content of phycoerythrin in porphyridium cruentum is improved and the phycoerythrin is separated and purified.

The invention belongs to the technical field of processing of health-care foods, and relates to a compound acanthopanax root preparation capable of improving activity of superoxide dismutase. A preparation method of the compound acanthopanax root preparation comprises the following steps of: adding 12g of acanthopanax root and 9g of thinleaf milkwort root-bark into 70 percent ethanol which is 10 times of the total amount of the acanthopanax root and the thinleaf milkwort root-bark for leaching at the room temperature for 10 hours, performing hermetic ultrasonic treatment for 30 minutes, filtering, and repeatedly extracting filter residues with two kinds of 70 percent ethanol, which are 6 times and 4 times of the total amount of the acanthopanax root and the thinleaf milkwort root-bark once respectively; combining filtrates and recovering ethanol to obtain acanthopanax root and thinleaf milkwort root-bark extracts; centrifuging 20g of porphyridium cruentum with a centrifugal machine for 15 minutes, suspending in 50 mmol / L of phosphoric acid buffer solution of which pH is 7.4, ultrasonically smashing, centrifuging by using a high-speed refrigerating centrifugal machine for 15 minutes to obtain a supernatant, and chilling and drying to obtain a dry substance; and uniformly mixing the dry substance with the acanthopanax root and thinleaf milkwort root-bark extracts to obtain the compound acanthopanax root preparation. The compound acanthopanax root preparation can be applied in the field of foods, medicaments and the like for developing a new natural antioxidant, and is beneficial to industrial production.

The invention discloses a giant salamander feed. The giant salamander feed comprises the following components in parts by weight: 10 to 20 parts of crab meat, 20 to 25 parts of fishbone dust, 5 to 15parts of compound amino acids, 5 to 10 parts of plantain herb, 5 to 10 parts of verbena officinalis, 10 to 15 parts of Emblica officinalis pulp, 5 to 10 parts of porphyridium cruentum, 5 to 10 parts of laurate, 10 to 20 parts of soybean meal and 5 to 15 parts of complex vitamins. The invention also discloses a preparation method of the feed. The preparation method comprises the following steps: (1) treatment of Emblica officinalis; (2) preparation of compound amino acids; (3) preparation of complex vitamins; (4) pretreatment of raw materials; and (5) pelleting to obtain the giant salamander feed. The formula of the giant salamander feed is easy to obtain, the giant salamander feed is free of toxic action and is low in price, and the preparation method of the feed is simple, high in operability and low in preparation cost and is suitable for wide popularization and application; the physiological effects such as the immunity of the organism can be improved, the giant salamander are prevented from being infected by viruses, the appetite of the giant salamanders can be increased through fruit fragrance, and rapid growth of the giant salamanders is promoted.

The invention belongs to the technical field of processing of health-care food, and relates to a compound Siberian solomonseal rhizome preparation capable of improving the vigor of superoxide dismutase. A method for preparing the compound Siberian solomonseal rhizome preparation comprises the following steps of: adding 70 percent ethanol in an amount which is 10 times that of 12 grams of Siberian solomonseal rhizome and 9 grams of largehead atractylodes rhizome, leaching at room temperature for 10 hours, performing hermetic ultrasound for 30 minutes, filtering, extracting filter residues once again from 70 percent ethanol in an amount which is 6 times and 4 times that of the filter residues respectively; mixing the filtrate, and recovering the ethanol to obtain a Siberian solomonseal rhizome extract and a largehead atractylodes rhizome extract; centrifuging 20 grams of porphyridium cruentum for 10 minutes by using a centrifugal machine, suspending in 50 mmol / L of phosphate buffer, of which the pH value is 7.4, performing ultrasonication, centrifuging for 15 minutes by using a high-speed freezing centrifugal machine to obtain supernate, and freezing and drying to obtain a dry substance; and mixing the dry substance with the Siberian solomonseal rhizome extract and the largehead atractylodes rhizome extract uniformly to obtain the compound Siberian solomonseal rhizome preparation. The compound Siberian solomonseal rhizome preparation can be used for developing new natural antioxidants in fields of food, medicines and the like and is favorable for industrial production.

The invention relates to the technical field of gene engineering, and particularly relates to a porphyridium chloroplast homologous recombination empty vector and application thereof. The vector comprises an upstream homologous arm, a downstream homologous arm, promoters and terminators, wherein a base sequence of the upstream homologous arm is shown in SEQ ID NO:1 of a porphyridium chloroplast gene group, a base sequence of the downstream homologous arm is shown in SEQ ID NO:2, base sequences of the promoters are shown in SEQ ID NO:3 and SEQ ID NO:4, base sequences of the terminators are shown in SEQ ID NO:5 and SEQ ID NO:6; and a corresponding base sequence which can form a multicistron structure with at least one exogenous gene and is shown in SEQ ID NO:7 is inserted between the corresponding promoter and the corresponding terminator. By adopting the porphyridium chloroplast homologous recombination empty vector disclosed by the invention, stable expression of multiple exogenous genes in the chloroplast can be realized.

Methods of use and compositions useful for moisturizing skin, conditioning skin, reducing lines and wrinkles, evening skin tone, lightening skin tone, increasing skin radiance, elasticity, skin barrier function, and / or skin firmness, reducing photodamage, reducing sagging skin, increasing anti-oxidant capacity in skin, MMP-1, MMP-3, MMP-9, pro-inflammatory cytokines (e.g., lipoxygenase, IL-6, Il-8, TNF-alpha, or VEGF), and / or elastase expression in skin, and / or increasing collagen expression in skin, elastin expression in skin, laminin expression in skin, and / or fibronectin expression in skin are disclosed herein. The composition includes a combination of one or more of Rosmarinus officianalis leaf extract, Caulerpa lentillifera extract, Morus alba fruit extract, and Porphyridium cruentum extract.

The invention discloses a method for increasing the yield of ARA (arachidonic acid) produced through fermentation of Mortierella alpina by adding porphyridium. According to the method, porphyridium residues are added to a fermentation culture medium so as to increase the yield of the ARA. After the Mortierella alpina is cultured on a culture-medium-rich PDA (potato dextrose agar) plate, a conical seed culture flask is inoculated with spores for culture for 36 h-48 h, the fermentation culture medium is inoculated with a seed solution with the inoculation amount of 5%-10% for continuous fermentation culture, and the treated porphyridium residues are added during fermentation culture; tests prove that the method can obviously increase the yield of the ARA produced from the Mortierella alpine, has the advantages of simplicity in operation, capability of reducing the fermentation cost and reducing pollution treatment and the like and can be easily applied to the industry.

The invention discloses a method and a device for co-producing polysaccharide through high-density semi-continuous culture of porphyridium, and belongs to the technical field of marine microorganisms. The device comprises a cylindrical bioreactor with an open upper end and a closed lower end, the upper end is connected with a carbon dioxide inlet and an air inlet through a three-way pipe, a main pipeline of the three-way pipe is communicated to the bottom of the bioreactor, and gas flow controllers and valves are arranged on a carbon dioxide inlet branch pipe. A water inlet is further formed in the upper end of the bioreactor, a liquid outlet is formed in the bottom side wall of the bioreactor, and the LED lighting device is composed of a transparent glass cover and at least one LED lamp panel and extends into the bioreactor. The porphyridium is semi-continuously cultured on a large scale through the device, meanwhile, the porphyridium polysaccharide is co-produced by controlling the feeding condition, the renewal rate and the renewal period of the culture medium in the semi-continuous culture process, the yield of the porphyridium polysaccharide is greatly improved compared with that of batch culture, and the device is suitable for large-scale production.