The invention relates to an aqueous two-phase extraction method for total
flavones in
kudzu root. The method comprises the following steps of: (1)
solid-liquid leaching: smashing a
kudzu root medicinal material, adding a leaching agent, heating, performing
reflux extraction, and combining the filtrate to obtain a
kudzu root crude extract; (2) aqueous two-phase extraction: concentrating the kudzu root crude extract obtained in the step (1), adding into an aqueous two-phase
system formed by absolute
ethanol /
monopotassium phosphate, adding water, mixing uniformly, and standing to separate into an upper phase and a lower phase; and (3) distilling under reduced pressure, and
drying in vacuum to obtain a kudzu root total flavone extract. The invention has the advantages: (1) phase separating israpid, and the
recovery rate is high; (2)
process integration is performed, the aim of purifying through aqueous two-phase extraction is fulfilled, and a material liquid is concentrated; (3) the use of n-butyl
alcohol serving as a toxic
solvent is avoided; (4) the boundary tension is small, and
mass transfer between two phases is facilitated; (5) purification of a sample is facilitated; and (6) process amplification and
continuous operation are easy, and a subsequent purification process can be directly connected without special treatment.