Aqueous two-phase extraction method for total flavones in kudzu root

A two-phase, total flavonoid technology, applied in pharmaceutical formulations, medical preparations containing active ingredients, plant raw materials, etc., can solve the problems affecting the progress of industrial-scale applications, low denaturation of biologically active substances, and the cost of polyethylene glycol. Advanced problems, to achieve the effect of easy process scale-up and continuous operation, rapid phase separation, and shortened experimental period

Inactive Publication Date: 2012-01-18
WUHAN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the polymer used is non-toxic, it has been included in the pharmacopoeia of many countries, and its polyol and polysaccharide structure can stabilize biological macromolecules. However, in practical applications, these two types of aqueous two-phase systems have their own advantages and disadvantages. The two systems have low denaturation effect on biologically active substances and less interface adsorption, but the cost of polyethylene glycol (PEG) and dextran (Dextran) used is too high, and the viscosity of the system is high, which affects the progress of industrial-scale applications. There are still very few examples of real industrialization

Method used

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  • Aqueous two-phase extraction method for total flavones in kudzu root
  • Aqueous two-phase extraction method for total flavones in kudzu root
  • Aqueous two-phase extraction method for total flavones in kudzu root

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1) Solid-liquid extraction

[0021] Take about 50.0 g of crushed kudzu root medicinal material, weigh it accurately, heat and reflux extraction with 70% ethanol in a 90°C water bath for 1 h, the solid-liquid ratio is 1 g: 10 ml, repeat 3 times, filter, and combine the filtrates to obtain The pueraria root crude extract is heated and concentrated to obtain an extract, and dispersed with water to 1 / 5 of the volume of the original filtrate to obtain a pueraria root sample solution;

[0022] 2) Aqueous two-phase extraction

[0023] Take the above Pueraria root sample solution, add dehydrated ethanol, potassium dihydrogen phosphate is 100 ml and 53 g respectively, add water, control the mass fraction of dehydrated alcohol and potassium dihydrogen phosphate in the ethanol / potassium dihydrogen phosphate phase diagram ( figure 1 ) above the curve, so that the mass fraction of anhydrous ethanol is 25%, the mass fraction of potassium dihydrogen phosphate is 17%, the pH is 5.8, a...

Embodiment 2

[0036] 1) Solid-liquid extraction

[0037] Take about 50.0 g of pulverized Pueraria lobata medicinal material, weigh it accurately, heat and reflux extraction with 70% ethanol in a water bath at 90°C for 1 h, the solid-liquid ratio is 1 g: 15 ml, repeat 3 times, filter, and heat and concentrate the filtrate to obtain The extract is dispersed with water to 1 / 5 of the volume of the original filtrate to obtain the pueraria root sample solution.

[0038] 2) Aqueous two-phase extraction

[0039] Take the above kudzu root sample solution, add absolute ethanol, potassium dihydrogen phosphate 100 ml and 55 g respectively, add water, wherein the mass fraction of absolute ethanol is 27.8%, the mass fraction of potassium dihydrogen phosphate is 19.4%, and the pH is 6.5, 30 Place at ℃ for 10 minutes, stand still and separate the phases. After calculation, the same as in Example 1, the yield of total flavonoids in the ethanol phase is 92.8%.

[0040] 3) Concentrated extract

[0041] The...

Embodiment 3

[0043] 1) Solid-liquid extraction

[0044] Take about 50.0 g of pulverized Pueraria lobata medicinal material, weigh it accurately, heat and reflux extraction with 70% ethanol in a 90°C water bath for 1 h, the solid-liquid ratio is 1 g: 20 ml, repeat 3 times, filter, and heat and concentrate the filtrate to obtain The extract is dispersed with water to 1 / 5 of the volume of the original filtrate to obtain the pueraria root sample solution.

[0045] 2) Aqueous two-phase extraction

[0046] Take the above kudzu root sample solution, add absolute ethanol, potassium dihydrogen phosphate 100 ml and 50 g respectively, add water, wherein the mass fraction of absolute ethanol is 28.3%, the mass fraction of potassium dihydrogen phosphate is 17.9%, and the pH is 5.3, 30 ℃ for 15 minutes, stand still and separate the phases. After calculation, with Example 1, the yield of total flavonoids in the ethanol phase is 87.2%.

[0047] 3) Concentrated extract

[0048] The anhydrous ethanol ph...

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Abstract

The invention relates to an aqueous two-phase extraction method for total flavones in kudzu root. The method comprises the following steps of: (1) solid-liquid leaching: smashing a kudzu root medicinal material, adding a leaching agent, heating, performing reflux extraction, and combining the filtrate to obtain a kudzu root crude extract; (2) aqueous two-phase extraction: concentrating the kudzu root crude extract obtained in the step (1), adding into an aqueous two-phase system formed by absolute ethanol / monopotassium phosphate, adding water, mixing uniformly, and standing to separate into an upper phase and a lower phase; and (3) distilling under reduced pressure, and drying in vacuum to obtain a kudzu root total flavone extract. The invention has the advantages: (1) phase separating israpid, and the recovery rate is high; (2) process integration is performed, the aim of purifying through aqueous two-phase extraction is fulfilled, and a material liquid is concentrated; (3) the use of n-butyl alcohol serving as a toxic solvent is avoided; (4) the boundary tension is small, and mass transfer between two phases is facilitated; (5) purification of a sample is facilitated; and (6) process amplification and continuous operation are easy, and a subsequent purification process can be directly connected without special treatment.

Description

technical field [0001] The invention belongs to the field of separation and purification of effective components of natural products, and in particular relates to a method for extracting total flavonoids in kudzu root with two aqueous phases. Background technique [0002] Pueraria lobata, also known as Luhuo and Jiqi, is the dried root of Pueraria mirifica or Pueraria puerariae. It is a commonly used Chinese medicinal material. It has been recorded in herbal medicines since ancient times. Warming the yang and stopping diarrhea, improving the body's immunity and other functions. At present, kudzu root is widely used to improve cardiovascular and cerebrovascular. In the important compound preparations for the treatment of diarrhea and diabetes, the dosage is extremely large, and it is an important component of the preparations. There are more than 140 kinds of kudzu root compound preparations included in the Pharmacopoeia of the People's Republic of China and the Ministry of...

Claims

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Application Information

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IPC IPC(8): A61K36/48A61P9/06A61P9/10A61P9/12
Inventor 池汝安张越非余军霞
Owner WUHAN INSTITUTE OF TECHNOLOGY
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