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Aqueous two-phase extraction and separation method for flavonoids, saponins and polysaccharides of astragalus

A technology of astragalus flavonoids and two-phase water, which is applied to medical preparations containing active ingredients, pharmaceutical formulas, plant/algae/fungus/moss components, etc., and can solve problems such as high raw material costs and difficulties in polymer recycling. Achieve the effects of saving reagents, easy process amplification and continuous operation, and low interfacial tension

Inactive Publication Date: 2012-10-03
WUHAN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional two-phase aqueous system has disadvantages such as high cost of raw materials and difficulty in recycling polymers.

Method used

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  • Aqueous two-phase extraction and separation method for flavonoids, saponins and polysaccharides of astragalus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Solid-liquid extraction

[0027] Take 1.0 g of crushed Radix Astragali, weigh it accurately, heat and reflux extraction with 20 mL of ethanol with a volume fraction of 60% in a water bath at 70 °C for 1 h, repeat 3 times and then filter, combine the filtrates, and after standing for 24 hours, the precipitate obtained by filtration is polysaccharide, the filtrate is the crude extract of Radix Astragali;

[0028] (2) Aqueous two-phase extraction

[0029] Take the crude extract of Radix Astragali above, concentrate to 1 / 5 of the original volume, add dipotassium hydrogen phosphate, ethanol and water to form a two-phase system, so that absolute ethanol accounts for 25% of the system mass fraction, and dipotassium hydrogen phosphate accounts for 25% of the system mass fraction. The mass fraction is 16%, mix well, keep the pH at 6.8, place at room temperature for 10 minutes, stand still and separate the phases, wherein the yield of total flavonoids in the upper phase of e...

Embodiment 2

[0049] (1) Solid-liquid extraction

[0050]Take 1.0 g of pulverized Radix Astragali, weigh it accurately, heat and reflux extraction with 20 ml of ethanol with a volume fraction of 70% in a water bath at 80°C for 1 h, repeat 3 times and then filter, combine the filtrates, and after standing for 48 hours, filter to obtain a precipitate: polysaccharide, the filtrate is crude extract of Radix Astragali;

[0051] (2) Aqueous two-phase extraction

[0052] Take the above crude extract of Astragalus membranaceus, concentrate to 1 / 5 of the original volume, add dipotassium hydrogen phosphate, ethanol and water to form a two-phase system, so that absolute ethanol accounts for 22% of the system mass fraction, and dipotassium hydrogen phosphate accounts for 22% of the system mass fraction. The mass fraction is 20%, mixed well, keeping the pH at 7.5, standing at room temperature for 10 minutes, standing still and separating the phases, wherein the yield of total flavonoids in the ethanol ...

Embodiment 3

[0057] (1) Solid-liquid extraction

[0058] Take 1.0 g of pulverized Radix Astragali, weigh it accurately, heat and reflux with 30 ml of ethanol with a volume fraction of 80% in a water bath at 90°C for 1 h, repeat 3 times and then filter, combine the filtrates, and after standing for 48 hours, filter to obtain a precipitate: polysaccharide, the filtrate is the crude extract of Radix Astragali;

[0059] (2) Aqueous two-phase extraction

[0060] Take the crude extract of Astragalus membranaceus, concentrate it to 1 / 5 of the original volume, add dipotassium hydrogen phosphate, ethanol and water to form a two-phase system, so that absolute ethanol accounts for 28% of the system mass fraction, and dipotassium hydrogen phosphate accounts for 28% of the system mass fraction. The mass fraction is 18%, the pH is kept at 8.2, placed at room temperature for 10 minutes, and the phases are separated, wherein the yield of total flavonoids in the ethanol phase is 87.2%, and the yield of sa...

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Abstract

The invention relates to an aqueous two-phase separation method for total flavonoids, saponins and polysaccharides in astragalus. The method comprises the following steps of smashing an astragalus medicinal material; adding a solvent and performing solid-liquid extraction; filtering and removing impurities to obtain crude extract of the total flavonoids and the saponins of the astragalus; taking and concentrating the crude extract of the astragalus; adding an aqueous two-phase system formed by absolute ethyl alcohol / dipotassium hydrogen phosphate, adding water to make the total amount of the system be a constant value; mixing uniformly and standing, wherein the aqueous two-phase system is divided into an upper phase and a lower phase; taking an ethanol phase which contains a large amount of total flavonoids and saponins of the astragalus out; and concentrating the ethanol phase under a reduced pressure to obtain the extract of the total flavonoids of the astragalus. The method has the beneficial effects that the extraction rate of the total flavonoids and the total saponins of the astragalus; simultaneously the total flavonoids and the total saponins can be separated from the polysaccharides of the astragalus in the crude extract; the used solvent is low in toxicity, favorable to environment friendliness and low in cost; the inactivation or the denaturation of the flavonoids and the saponins of the astragalus cannot be caused; the phase separation time is short; the interfacial tension is low; and residual organic solvent does not exist.

Description

technical field [0001] The invention belongs to the field of separation and purification of effective components of natural products, and relates to a method for separating astragalus flavonoids, saponins and polysaccharides by two-phase extraction. Background technique [0002] Astragalus membranaceus is the dried root of the leguminous plant Astragalus membranaceus or Astragalus membranaceus. The main active ingredients in Astragalus are flavonoids, saponins and polysaccharides. The flavonoids mainly include formononetin, ononion, calycosin and their glycosides. Studies have confirmed that the total flavonoids of astragalus have the ability to scavenge O 2- · and OH- prevent biofilm peroxidation, and can also significantly inhibit OH- damage to DNA. It is the main component of Astragalus anti-oxidation, which is manifested as significant anti-hepatotoxicity, anti-inflammation, antibacterial and heart protection. The role of the vascular system. Astragalus total saponin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/481C08B37/00A61P1/16A61P9/10A61P31/12A61P29/00A61P35/00A61P37/02A61P39/06
Inventor 池汝安张越非钟玲余军霞徐志高肖春桥
Owner WUHAN INSTITUTE OF TECHNOLOGY
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