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Method and device for co-producing polysaccharide through high-density semi-continuous culture of porphyridium

A Porphyridium, semi-continuous technology, applied in the field of marine microorganisms, can solve the problems of undetermined biochemical variation, singleness, lack, etc., and achieve the effect suitable for large-scale production

Pending Publication Date: 2022-03-01
广西源藻生物科技有限公司
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Problems solved by technology

Due to the simple operation of semi-continuous culture, the production and biochemical composition are generally controlled mainly from parameters such as renewal rate and nutrient concentration, and changes in cultivation parameters such as renewal rate and nutrient concentration have certain effects on the biochemical variation of Porphyria coccus, but the existing The method of controlling yield and biochemical composition by changing cultivation parameters such as renewal rate or nutrient concentration is too simple to determine its biochemical variation, and there is a lack of an efficient method for high-density semi-continuous cultivation of Porphyridum algae to produce polysaccharides

Method used

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  • Method and device for co-producing polysaccharide through high-density semi-continuous culture of porphyridium
  • Method and device for co-producing polysaccharide through high-density semi-continuous culture of porphyridium
  • Method and device for co-producing polysaccharide through high-density semi-continuous culture of porphyridium

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Embodiment 1

[0033] Pre-cultivate the Porphyridum seed liquid in OMII medium first, inoculate the normal cultured to the end of the logarithmic phase of the Porphyridum seed liquid into the OMⅡ medium in the bioreactor to expand the culture, and control the initial Porphyridum cell inoculation amount 6×10 5 a / L. Culture conditions: culture temperature is 25°C and pH 6.5 is monitored and adjusted in time by the thermometer and pH meter installed on the top of the bioreactor, the light source is illuminated by LED cool white light, and the light intensity is adjusted to 80μE.m -2 .s -1 1% carbon dioxide is introduced through the ventilation pipe extending to the bottom of the bioreactor, and the ventilation rate is 10L / min. After 7 days of cultivation under the above-mentioned cultivation conditions, the semi-continuous cultivation begins when Porphyridium enters the logarithmic phase, and the cultivation is carried out according to OMⅡ NaNO 3 The concentration of each feed was 0.5g / L for...

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Abstract

The invention discloses a method and a device for co-producing polysaccharide through high-density semi-continuous culture of porphyridium, and belongs to the technical field of marine microorganisms. The device comprises a cylindrical bioreactor with an open upper end and a closed lower end, the upper end is connected with a carbon dioxide inlet and an air inlet through a three-way pipe, a main pipeline of the three-way pipe is communicated to the bottom of the bioreactor, and gas flow controllers and valves are arranged on a carbon dioxide inlet branch pipe. A water inlet is further formed in the upper end of the bioreactor, a liquid outlet is formed in the bottom side wall of the bioreactor, and the LED lighting device is composed of a transparent glass cover and at least one LED lamp panel and extends into the bioreactor. The porphyridium is semi-continuously cultured on a large scale through the device, meanwhile, the porphyridium polysaccharide is co-produced by controlling the feeding condition, the renewal rate and the renewal period of the culture medium in the semi-continuous culture process, the yield of the porphyridium polysaccharide is greatly improved compared with that of batch culture, and the device is suitable for large-scale production.

Description

technical field [0001] The invention belongs to the technical field of marine microorganisms, and in particular relates to a method and a device for high-density semi-continuous culture of Porphyridum algae for co-production of polysaccharides. Background technique [0002] Porphyridium purpureum belongs to the single-celled marine microalgae of Rhodophyta, which has strong environmental adaptability and rapid reproduction ability, and can naturally and efficiently accumulate phycoerythrin, sulfated polysaccharides and polyunsaturated fatty acids (Polyunsaturated fatty acids, PUFAs) and other bioactive ingredients, naturally rich in omega-6 series (such as linoleic acid LA and arachidonic acid ARA) and omega-3 series (such as linolenic acid ALA and EPA) polyunsaturated fatty acids. Under normal growth conditions, the intracellular oil content of Porphyridium algae is about 15%–20%, the total protein content is about 40%, and the polysaccharide content is about 25%. A large ...

Claims

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Application Information

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IPC IPC(8): C12M1/04C12M1/36C12M1/34C12M1/00C12N1/12C12P19/04F21V3/06F21V19/00F21V33/00C12R1/89F21Y115/10
CPCC12M21/02C12M23/22C12M29/26C12M41/12C12M41/34C12M41/26C12N1/12C12P19/04F21V3/061F21V19/003F21V33/00F21Y2115/10
Inventor 王长海刘瑞卿何梅琳王烨
Owner 广西源藻生物科技有限公司
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