Application of compound 3,3',5,5'-tetrabromo-2,2'-biphenyldiol in inhibiting and removing algae
A biquinol and compound technology is applied in the new application field of the compound, which can solve the problems of hypoxia death, pollution of the marine environment, destruction of the normal marine ecosystem, etc., and achieve a good inhibitory effect.
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Embodiment 1
[0042] Embodiment 1 Calculation method of anti-algae activity
[0043] 1. Tested algal species and culture conditions
[0044] Synechococcus sp. PCC7002 was selected as the tested algae species, and cultured at a temperature of 26°C, a light intensity of 3000lx, and a light-dark cycle of 12h:12h. After being cultivated to the exponential phase, it was then divided into 50mL triangular flasks, and each bottle was filled with 20mL of algae. liquid.
[0045] 2. Calculation of algae inhibition rate
[0046] Take 1 mL of the component to be tested at 10 μg / mL and add it to the above-mentioned Synechococcus PCC7002 algae liquid, culture for 5 days, and then count under a fluorescence microscope.
[0047] The algae inhibition rate is calculated according to the following formula
[0048] Algae inhibition rate (Algicidal rate) = (N C -N T ) / N C ×100%
[0049] N C Indicates the number of viable cells in the control group, N T Indicates the number of viable cells in the experim...
Embodiment 2
[0051] The scope of inhibiting algae of this compound of embodiment 2
[0052]Selection of Thalassiosira pseudodwarf, Chaetoceros mourii, Prorocentrum microphylla, Angiosperm forta, Nannochloropsis, Prochlorococcus, Dunaliella salina, Phaeodactylum tricornutum, Chlorella, subcardiac The algicidal spectrum experiment of 3,3',5,5'-tetrabromo-2,2'-biphenyldiol was carried out by Pyrenella spp. The culture conditions are: temperature 20°C, light intensity 3000lx, light-dark cycle 12h:12h. Take 10 μg / mL of 3,3',5,5'-tetrabromo-2,2'-biphenyldiol and add it to the above-mentioned 11 algae liquids at a ratio of 5%, and inhibit algae according to the method in Example 1. Rate calculation formula to calculate algae inhibition rate.
[0053] The experimental results showed that 3,3',5,5'-tetrabromo-2,2'-biphenyldiol was effective against Thalassiosira pseudodwarf, Chaetoceros mourii, Prorocentrum minutiae, Amphidinium forta, Coccus, Prochlorococcus, and Dunaliella salina showed strong...
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