Compound mulberry preparation capable of improving superoxide dismutase activity
A technology of superoxide and dismutase, which can be used in medical preparations containing active ingredients, antidote, food science, etc., and can solve problems such as side effects
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Embodiment 1
[0019] 1, a kind of preparation method of the compound mulberry preparation that can improve superoxide dismutase activity is characterized in that comprising the following steps:
[0020] (1) The weight ratio of raw materials is: mulberry fruit 9g, medlar fruit 6g, Porphyridum 10g;
[0021] (2) Add 8 times the amount of 70% ethanol to extract 8 times the amount of mulberry and medlar at room temperature, then seal and ultrasonicate for 30 minutes at a temperature of 30°C and a power of 80W, then filter with gauze, and use 6 times and 4 times the amount of filter residues respectively Repeat the extraction with 70% ethanol once, and filter to obtain the filtrate;
[0022] (3) The filtrate in (2) is merged, reclaims ethanol, obtains mulberry fruit, wolfberry extract;
[0023] (4) Centrifuge Porphyridium algae with a 5000r / min centrifuge for 10min, then suspend it in pH 7.4, 50mmol / L phosphate buffer, perform ultrasonic crushing, break for 5s, interval 5s, 20 times, use 10000r / ...
Embodiment 2
[0027] 1, a kind of preparation method of the compound mulberry preparation that can improve superoxide dismutase activity is characterized in that comprising the following steps:
[0028] (1) The weight proportion of raw material medicine is: 12g of mulberry fruit, 9g of medlar, 20g of Porphyridium coccus;
[0029] (2) Add 10 times the amount of 70% ethanol to mulberry fruit and medlar and extract at room temperature for 10 hours, then seal and ultrasonicate for 30 minutes at a temperature of 30°C and a power of 80W, then filter with gauze, and use 6 times and 4 times the amount of filter residues respectively Repeat the extraction with 70% ethanol once, and filter to obtain the filtrate;
[0030] (3) The filtrate in (2) is merged, reclaims ethanol, obtains mulberry fruit, wolfberry extract;
[0031] (4) Centrifuge Porphyridium algae with a 5000r / min centrifuge for 10min, then suspend it in pH 7.4, 50mmol / L phosphate buffer, perform ultrasonic crushing, break for 5s, interva...
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