132 results about "Superoxide dismutase activity" patented technology

The invention discloses a method for screening corn salt / alkali-tolerance germplasm, which belongs to the field of crop cultivation; the method comprises the following steps of: firstly putting a batch of corn seeds in a culture dish and testing the germination rate thereof; taking another batch of corn seeds for germination, culturing the seeds in a greenhouse, and stressing the seedlings when the seedlings grow to the three-leaf and one-core stage; taking the leaves of the seedlings to be tested, and testing the relative conductivity, the SOD activity, the MDA content, and the proline content; comparing the salt / alkali tolerances of the corn seeds. The method provided by the invention can complete the screening of the corn germplasm in a laboratory, in short time and with low cost.

The present invention relates to stilbene glycosie compound and the use of its derivative and medicinal salt in preparing medicine for dementia, especially for Alzheimer disease and vascular dementia. Animal experiment proves that stilbene glycoside compound can resist brain cell damage caused by beta-amyloid protein and hydrogen peroxide, raise the memory capacity of mouse model with scopolaminedementia and A beta dementia and gerbille model with double-sided common carotid artery obstruction caused brain ischemia, inhibit the lipide peroxidation of brain tissue of mouse model with brain ischemia, strengthen the activity of its brain tissue SOD, and reduce the calcium ion density in its brain cells.

The invention relates to a green plum enzyme and a production process thereof. The green plum enzyme comprises the following raw materials in weight percent: 15-25% of green plum, 15-25% of grape, 10-20% of cranberry, and 10-25% of longan meat , mulberry 10-20%, wheat germ powder 10-20%, polylysine 4-8% and ascorbic acid 4-8%. The green plum enzyme described in the present invention uses various pure natural materials as raw materials, and is obtained after processes such as enzymolysis, homogenization, and probiotic fermentation. efficacy and food safety. The green plum enzyme contains higher resveratrol content and superoxide dismutase enzyme activity, which can reduce the risk of atherosclerosis and coronary heart disease. The green plum enzyme of the invention has a simple preparation process, shortens the natural fermentation time and product production cycle, and has important application value.

The invention provides application of guluronic acid oligomers in preparing medicines, health products or dietary supplements for preventing and treating alcoholic liver injury. Guluronic acid oligomers with different molecular weights, which are obtained by degrading polyguluronic acid graded and purified from algin, can lower the hepatosomatic index increase caused by alcoholic injury, resist triglyceride and cholesterol increase caused by alcohol, effectively lower glutamic-pyruvic transaminase and glutamic oxaloacetic transaminase increase in serum caused by alcohol, enhance the total oxidation resistance, glutathione level and superoxide dismutase activity in the serum, inhibit the generation of malonaldehyde in the liver caused by alcohol, lower the mouse drunkenness rate, prolong the mouse drunkenness time, and shorten the sober-up time, thereby implementing the protective action and hangover alleviating function on alcoholic hepatic injury. The products provided by the invention are derived from marine natural products, have the advantages of abundant resources, high industrialization tendency, high safety, high effectiveness and the like, and have wide development and application prospects in the aspects of prevention and treatment of alcoholic hepatic injury, hangover alleviation and liver protection.

The invention provides a method for measuring activity of superoxide dismutase. The technical problems of detection difficulty, low accuracy and low reproducibility existing in a method for measuring the activity of superoxide dismutase in the prior art are solved. The method comprises the following steps: selecting a pyrogallic acid alkaline auto-oxidation system, evaluating the removal effect of the superoxide dismutase on superoxide anion by combining an ultraviolet-visible light spectrophotometric method, detecting change of absorbance of a product water-soluble formazan of a trapping agent of sodium 2-(4-iodobenzene)-3-(4-nitrobenzene)-5-(2',4-bisulfophenyl)-2H-tetrazole after acting with the superoxide anion, calculating the removal rate of the superoxide dismutase on the superoxide anion, and further obtaining the activity of superoxide dismutase. The method has the advantages of high reproducibility, high accuracy and simplicity and convenience in operation.

The invention discloses phyllanthus emblica fine powder containing high-activity superoxide dismutase and a preparation method. The phyllanthus emblica fine powder is prepared by juicing, purifying, concentrating and drying the raw materials, namely phyllanthus emblica fresh fruits, wherein the activity of superoxide dismutase is 0.45-0.55 million U / 100g. The method specifically comprises the following steps of: raw material treatment: selecting decay-free and mildew-free phyllanthus emblica fresh fruits without exogenous impurities which can be seen with naked eyes and grinding the phyllanthus emblica fresh fruits until the grain sizes are 3-5mm after washing the phyllanthus emblica fresh fruits with running water; juicing: juicing the ground phyllanthus emblica and using the juice obtained after removing the fruit residues later; purifying: colating the fruit to remove the fruit residues and other impurities and carrying out high-speed centrifugal separation on the filtrate to obtain separation liquid; concentrating: carrying out vacuum concentration on the separation liquid until the relative density is 1.0-1.3; and drying: drying the concentrates to obtain the phyllanthus emblica fine powder containing high-activity superoxide dismutase. In the steps of concentrating and drying, the lower temperature at 40-50 DEG C is adopted, thus ensuring that the activities of superoxide dismutase and Vc are not easily damaged.

The invention belongs to the technical field of processing of health-care products and relates to a compound mulberry preparation capable of improving superoxide dismutase activity. The preparation method for the compound mulberry preparation comprises the following steps of: extracting 12g of mulberry and 9g of medlar in ethanol of which the concentration is 70 percent and the amount is 10 timesthat of the mulberry and the medlar at room temperature for 10 hours, sealing and crushing the extract with ultrasonic waves for 30 minutes, filtering the extract, and extracting the filtration residue with ethanol of which the concentration is 70 percent and the amount is 6 times and 4 times that of the filtration residue again for one time respectively; combining the filtrates, reclaiming the ethanol to obtain an extract of the mulberry and medlar; centrifuging 20g of porphyridium cruentum with a centrifugal for 10 minutes, suspending the porphyridium cruentum in 50mmol / L of phosphate buffer of which the pH value is 7.4, crushing the porphyridium cruentum with ultrasonic waves, centrifuging the crushed porphyridium cruentum with a high-speed centrifugal for 15 minutes to obtain a liquidsupernatant, and refrigerating and drying the liquid supernatant to obtain a dried product; and uniformly mixing the dried product and the extract of the mulberry and medlar to obtain the mulberry preparation. The mulberry preparation can be applied to a natural antioxidant which is newly developed in fields, such as food, medicament and the like, and contributes to industrial production.

The invention provides a fresh-keeping agent for cut gerbera flowers. Based on 1L, the fresh-keeping agent comprises the following effective components in part by weight: 20-30g of sucrose, 40-120mg of 8-hydroxyquinoline, 60-180mg of citric acid, 1-5mg of 6-benzyladenine. The fresh-keeping agent provided by the invention has the advantages that in order to prolong the lives of the cut flowers and improve the quality of the cut flowers, the fresh-keeping agent containing a plant-growth regulating agent is used for carrying out bottle planting test, the fresh-keeping effect of the plant-growth regulating agent is researched by analyzing all bottle-planting indexes and physiological-biochemical indexes, and the result shows that the fresh-keeping agent containing the 6-benzyladenine has the effects of prolonging the lives of the cut flowers, increasing the change rate of fresh weight of the cut flowers, improving the water balance, adding soluble sugar and protein in pedals of the cut flowers, adjusting the content of proline and the activity of superoxide dismutase and improving the permeability of cell membranes.

The invention provides an application of natural attapulgite as a natural nano mineral enzyme, and belongs to the technical field of enzyme catalysis. The result of the embodiment shows that the natural attapulgite has peroxidase-like activity, catalase-like activity or superoxide dismutase-like activity, and has good biocompatibility. Compared with protease, the natural attapulgite has the advantages of being large in reserve, easy to obtain, low in cost, resistant to high temperature, wide in pH value range and the like; compared with researched and developed artificial nano-enzyme, the natural attapulgite has the advantages of being multifunctional, natural, non-toxic (from nature and free of heavy metals), good in biocompatibility, easy to obtain, free of complex processing treatment,capable of providing a growing and proliferating place for cells due to the huge surface area and the like.

A method for increasing the activity of superoxide dismutase comprises steps of: liquid culture, by providing a strain of Bacillus subtilis and a liquid medium to carry out a fed-batch fermentation and to obtain a primary fermentation broth, wherein the liquid medium has 8 wt % to 10 wt % of maltose, 10 wt % to 15 wt % of soya powder and rest of water; and solid culture, by culturing the primary fermentation broth in a solid medium, which has solid substrate and maltose in a ratio of 10:1 to 20:1, to obtain a secondary fermentation broth with SOD in high activity, wherein the solid substrate contains wheat germ and water.

The invention provides a method for extracting superoxide dismutase with a high yield from corn. The method comprises the following steps of: corn slurry-adding copper salt and separating-membrane filtering-precipitating organic liquid-drying-dissolving-conducting chromatography-freezing and drying and the like. According to the method, the copper salt is adopted to improve about 20% of activity of superoxide dismutase, and copper ion damage caused by the copper salt can be removed by means of subsequent membrane filtering as well as precipitating organic liquid for many times and drying. According to the invention, almost impurities in SOD (Superoxide Dismutase) are removed by combined steps of precipitating-drying-dissolving for multiple times by using time difference when superoxide dismutase and almost impurities (heteroprotein, nucleic acid and soluble starch) are completely dissolved in water, so that the purity is improved.

The invention provides a determination method for the activity of superoxide dismutase in tobacco leaves damaged by pesticides. The determination method comprises the following steps that 1, reagents are prepared, wherein the phosphate buffer, the extracting medium, the methionine solution, NBT, EDTA-Na2 and the riboflavin solution are prepared; 2, an enzyme solution is prepared from the tobacco leaves; 3, the activity of a superoxide dismutase sample is determined; 4, a tobacco is transplanted into pesticidal soil of which the quinclorac adding concentrations are 1.04*10<-3> mg / kg, 2.08*10<-3> mg / kg, 4.17*10<-3> mg / kg, 8.33*10<-3> mg / kg and 1.67*10<-2> mg / kg in the period that the tobacco has 5-6 true leaves, and the activity of the superoxide dismutase in the tobacco leaves is measured on the 7 day, the 14 day, the 21 day, the 28 day and the 35 day by adopting the step 1 to the step 3 separately. Accordingly, the problems that in the prior art, few influences that the SOD is subjected to and few changes of the SOD in the tobacco leaves damaged by quinclorac are known, and determination is not precise enough are solved.