132 results about "Superoxide dismutase activity" patented technology

The invention provides an antioxidation feedstuff for improving the immunity of sturgeons, which relates to an antioxidation feedstuff. The antioxidation feedstuff solves the problems that the sturgeons bred manually with the prior feedstuff have low immunity, are easy to be infected, are hard to cure with common drugs after catching a disease, and are sensitive to the common drugs. The antioxidation feedstuff for improving the immunity of the sturgeons is prepared by mixing fish meal, bean pulp, wheat middling, fish oil, soybean lecithin, aloe powder, cellulose, zeolite and a premix. The antioxidation feedstuff for improving the immunity of the sturgeons does not have obvious influence on the production performance of the sturgeons, obviously improves the blood plasma superoxide dismutase activity (P is less than 0.05), obviously reduces the blood plasma malonaldehyde activity (P is less than 0.05), improves the immunity of the sturgeons, and preventing the sturgeons from being infected and catching diseases.

Provided herein are diagnostic and prognostic methods, diagnostic and prognositic markers, and methods for evaluating anti-inflammatory agents or drugs in subjects with asthma and / or an analogous disease associated with high oxidative and nitrative stress at the disease site. In certain embodiments, the methods comprise a step of assaying for decreased levels of superoxide dismutase activity in the blood, serum, or plasma of the subject. In certain embodiments, the methods comprise a step of assaying for elevated levels of one or more oxidatively-modified SOD isoforms or species in the blood, serum or plasma of the subject. Also provided are diagnostic kits for use in the present invention. In certain embodiments, such kits comprise at least one binding reagent that specifically binds to at least one oxidatively-modified SOD species.

The invention belongs to the field of environmental toxicology, and relates to a method for testing typical antibiotics wastewater toxicity by using zebra fishes. According to the method, zebra fishes are used as to-be-tested organisms, and are exposed in typical antibiotics wastewater, the short-term or long-term toxic effect of the typical antibiotics wastewater to the zebra fishes is quantitatively tested through analyzing the acute toxicity and subacute toxicity of the typical antibiotics wastewater to the zebra fishes, the acute toxicity is described by using the 96h medium lethal concentration (96h LC50) (typical antibiotics wastewater percent by volume percent), and the subacute toxicity is described by fish body biochemical indexes such as superoxide dismutase (SOD) activity, peroxidase (POD) activity and malondialdehyde (MDA) content. By adopting the method, the toxicity characteristics and toxicity levels of the typical antibiotics wastewater can be analyzed, tested and quantitatively described, and the problem that conventional physicochemical indexes can not reflect biotoxicity of the typical antibiotics wastewater can be solved, and results obtained by analyzing, testing and describing can be used as biotoxicity monitoring and evaluating indexes of the typical antibiotics wastewater.

The invention provides an application of rhodium sheet nano enzyme in simulation of enzymatic catalysis. According to the application of the rhodium sheet nano enzyme in simulation of enzymatic catalysis, compared with a common rhodium catalytic hydrogenation reaction, the invention develops a catalysis application of the brand new rhodium sheet nano enzyme, the rhodium sheet nano enzyme has fourcatalytic activities of high simulation peroxidase activity, simulation oxide enzyme activity, simulation superoxide dismutase activity, and simulation catalase activity, the rhodium sheet nano enzymehas a wide pH value and a temperature usage scope, and can be adapted to various complex environment conditions of catalysis and detection, is benefit for a further development application scope, canbe used as the novel multifunctional nano enzyme for the fields of immunization analysis, biochemical detection and clinical diagnosis, and has very good market prospect and high application value.

The invention provides a Fe3O4 nanozyme with improved catalytic activity, and a preparation method and application thereof. By doping metal elements in Fe3O4 nanozyme, the dismutase activity of peroxidase, catalase and / or superoxide can be improved; the metal element doped Fe3O4 nanozyme can be prepared in large quantities, has low cost, good stability at room temperature, good safety, and good economic value and application prospect.

The orally taken antioxidant and antilipemic medicines include solid orally taken preparation prepared with forsythin and solid excipient in the weight ratio of 0.01-4 through mixing and conventionaltechnological process, and liquid orally taken preparation prepared with forsythin and liquid excipient in the weight ratio of 0.003-0.01 through mixing and conventional technological process. Test shows that forsythin as the active component of the present invention has excellent effect of clearing away hydroxy radical and can inhibit the oxidizing damage in tissue and cell organ level. The medicine of the present invention has also functions of delaying senility and reducing blood fat, etc.

The invention provides a Chinese herbal medicine fodder additive for accelerating crucians to grow and enhancing the immunity and a fodder containing the additive. The Chinese herbal medicine fodder additive is formed by drying malts, hawthorns, green teas, lotus leaves, radix ophiopogonis and semen cassiae, and then performing crushing and uniform mixing. The Chinese herbal medicine fodder additive is added into a common crucian fodder so that the growing speed of the crucians can be obviously improved, the water content of muscles is reduced, the protein content of the muscles is improved and the fat content of the muscles is reduced; the hepatosomatic index can be reduced, and the spleen index and the head-kidney index can be improved; the blood serum T-SOD (Total-Superoxide Dismutase)can be obviously improved and the content of MDA (Malondialdehyde), TG (Triglyceride) and TC (cholesterol) is obviously reduced; the Chinese herbal medicine fodder additive has the characteristics ofgreenness, naturalness, no drug resistance, no residues, diversified functions, consideration of growth and disease resistance and the like; and the Chinese herbal medicine fodder additive can greatly reduce the breeding cost of the crucians and is suitable for industrial production.

The invention discloses a method for screening corn salt / alkali-tolerance germplasm, which belongs to the field of crop cultivation; the method comprises the following steps of: firstly putting a batch of corn seeds in a culture dish and testing the germination rate thereof; taking another batch of corn seeds for germination, culturing the seeds in a greenhouse, and stressing the seedlings when the seedlings grow to the three-leaf and one-core stage; taking the leaves of the seedlings to be tested, and testing the relative conductivity, the SOD activity, the MDA content, and the proline content; comparing the salt / alkali tolerances of the corn seeds. The method provided by the invention can complete the screening of the corn germplasm in a laboratory, in short time and with low cost.

The present invention relates to stilbene glycosie compound and the use of its derivative and medicinal salt in preparing medicine for dementia, especially for Alzheimer disease and vascular dementia. Animal experiment proves that stilbene glycoside compound can resist brain cell damage caused by beta-amyloid protein and hydrogen peroxide, raise the memory capacity of mouse model with scopolaminedementia and A beta dementia and gerbille model with double-sided common carotid artery obstruction caused brain ischemia, inhibit the lipide peroxidation of brain tissue of mouse model with brain ischemia, strengthen the activity of its brain tissue SOD, and reduce the calcium ion density in its brain cells.

The invention relates to a green plum enzyme and a production process thereof. The green plum enzyme comprises the following raw materials in weight percent: 15-25% of green plum, 15-25% of grape, 10-20% of cranberry, and 10-25% of longan meat , mulberry 10-20%, wheat germ powder 10-20%, polylysine 4-8% and ascorbic acid 4-8%. The green plum enzyme described in the present invention uses various pure natural materials as raw materials, and is obtained after processes such as enzymolysis, homogenization, and probiotic fermentation. efficacy and food safety. The green plum enzyme contains higher resveratrol content and superoxide dismutase enzyme activity, which can reduce the risk of atherosclerosis and coronary heart disease. The green plum enzyme of the invention has a simple preparation process, shortens the natural fermentation time and product production cycle, and has important application value.

The invention relates to preparation and application methods of penaeus monodon fermented feed. The preparation method comprises the following steps: respectively carrying out enlarged culture on bacillus licheniformis, lactobacillus plantarum and candida utilis, then mixing in a volume ratio of 1:1:1, adding brown sugar (2g / 1000mL) and running water into the mixed bacterial solution under the condition that the ratio of the culture solution to water is 1:80, uniformly mixing, uniformly stirring with penaeus monodon compound feed in a ratio of 2:5, sealing with a plastic film, and fermenting for 18-28 hours, so that the fermented feed is obtained. The invention also provides the application method of the fermented feed. The prepared fermented feed is used for feeding penaeus monodon once a day, and the feeding amount is 20-30% of the daily total feeding amount. The prepared fermented feed can obviously improve nitric oxide synthase activity, superoxide dismutase activity, catalase activity, phenoloxidase activity, glutathione peroxidase activity and total antioxidant capacity of penaeus monodon and obviously reduce the malondialdehyde content; and composition of florae in intestines of the penaeus monodon is obviously improved, and the survival rate of penaeus monodon culture is obviously increased.

The invention discloses a technology for extracting proteins and superoxide dismutase (SOD) from horseradish tree leaves. The technology comprises the following steps: smashing and pulping fresh horseradish tree leaves, and performing digestion, pressure filtering, isoelectric precipitation and centrifugation to obtain precipitates and supernate; performing modification protection, thermal denaturation reaction and freeze drying on the precipitates to obtain SOD freeze-dry powder; performing isoelectric precipitation, centrifugation and spray drying on the supernage to obtain leaf protein powder. By the adoption of the technical methods such as normal-temperature digestion, isoelectric precipitation, centrifugal separation, the original functional characteristics of the proteins and the activity of enzyme are retained to the maximum extent, and the yield of the proteins and that of active enzyme are greatly increased; the obtained leaf protein powder is bright in color, high in protein content and stable in quality; the obtained SOD is high in activity, and the practical value and the economical benefit are higher. The technology is easy to operate, low in production cost, safe, reliable and suitable for industrial production.

The invention provides application of guluronic acid oligomers in preparing medicines, health products or dietary supplements for preventing and treating alcoholic liver injury. Guluronic acid oligomers with different molecular weights, which are obtained by degrading polyguluronic acid graded and purified from algin, can lower the hepatosomatic index increase caused by alcoholic injury, resist triglyceride and cholesterol increase caused by alcohol, effectively lower glutamic-pyruvic transaminase and glutamic oxaloacetic transaminase increase in serum caused by alcohol, enhance the total oxidation resistance, glutathione level and superoxide dismutase activity in the serum, inhibit the generation of malonaldehyde in the liver caused by alcohol, lower the mouse drunkenness rate, prolong the mouse drunkenness time, and shorten the sober-up time, thereby implementing the protective action and hangover alleviating function on alcoholic hepatic injury. The products provided by the invention are derived from marine natural products, have the advantages of abundant resources, high industrialization tendency, high safety, high effectiveness and the like, and have wide development and application prospects in the aspects of prevention and treatment of alcoholic hepatic injury, hangover alleviation and liver protection.

The invention relates to application of oligomeric proantho cyanidins in preparing products for preventing, diagnosing, detecting, protecting, treating and studying vascular dementia and directly related diseases thereof, in particular to new application in medicament and health-care products. Experiments prove that oligomeric proantho cyanidins can improve the study memory ability of a heart and brain ischemia-reperfusion mouse and a permanent bilateral common carotid arteries rat, inhibit lipid peroxidation in brains of the heart and brain ischemia-reperfusion mouse and the permanent bilateral common carotid arteries rat, and improve the superoxide dismutase enzyme activity of the heart and brain ischemia-reperfusion mouse and the permanent bilateral common carotid arteries rat. The oligomeric proantho cyanidins have strong action, clear effect, rich resource, simple preparation process, high yield, stable property and safe use, in particular one object with multiple functions and wide application range, and can play roles furthest. Disease rate of dementia rises gradually, after popularization, the application of oligomeric proantho cyanidins can generate significant social benefit and economic benefit in short time.

The invention discloses a holothurian regulation type feed additive, which is prepared from the following ingredients in percentage by weight: 3 to 10 parts of Shenqu, 5 to 13 parts of malt, 8 to 15 parts of hawthorn, 9 to 18 parts of cardenia, 10 to 20 parts of milk veteh, 15 to 25 parts of beer yeast, 2 to 8 parts of honeysuckle, 5 to 10 parts of dried orange peel, 10 to 18 parts of liquorice and 2 to 7 parts of cumin. A preparation method has the following steps: pulverizing all ingredients into 150 to 200 meshes; uniformly mixing and stirring the ingredients by a feed mixing machine; and then, adding the feed additive into holothurian feed for material feeding use after being mixed uniformly. The invention can promote the ingestion and the digestion of the holothurian, can improve the immunity and the growth of the holothurian, and is safe without noxious effect and residual, the percentage of liveweight growth of the holothurian can be improved by 7.6 percent to 11 percent, the activity of the superoxide dismutase can be improved by 10 percent to 25.6 percent.

The invention provides a method for measuring activity of superoxide dismutase. The technical problems of detection difficulty, low accuracy and low reproducibility existing in a method for measuring the activity of superoxide dismutase in the prior art are solved. The method comprises the following steps: selecting a pyrogallic acid alkaline auto-oxidation system, evaluating the removal effect of the superoxide dismutase on superoxide anion by combining an ultraviolet-visible light spectrophotometric method, detecting change of absorbance of a product water-soluble formazan of a trapping agent of sodium 2-(4-iodobenzene)-3-(4-nitrobenzene)-5-(2',4-bisulfophenyl)-2H-tetrazole after acting with the superoxide anion, calculating the removal rate of the superoxide dismutase on the superoxide anion, and further obtaining the activity of superoxide dismutase. The method has the advantages of high reproducibility, high accuracy and simplicity and convenience in operation.

The invention discloses phyllanthus emblica fine powder containing high-activity superoxide dismutase and a preparation method. The phyllanthus emblica fine powder is prepared by juicing, purifying, concentrating and drying the raw materials, namely phyllanthus emblica fresh fruits, wherein the activity of superoxide dismutase is 0.45-0.55 million U / 100g. The method specifically comprises the following steps of: raw material treatment: selecting decay-free and mildew-free phyllanthus emblica fresh fruits without exogenous impurities which can be seen with naked eyes and grinding the phyllanthus emblica fresh fruits until the grain sizes are 3-5mm after washing the phyllanthus emblica fresh fruits with running water; juicing: juicing the ground phyllanthus emblica and using the juice obtained after removing the fruit residues later; purifying: colating the fruit to remove the fruit residues and other impurities and carrying out high-speed centrifugal separation on the filtrate to obtain separation liquid; concentrating: carrying out vacuum concentration on the separation liquid until the relative density is 1.0-1.3; and drying: drying the concentrates to obtain the phyllanthus emblica fine powder containing high-activity superoxide dismutase. In the steps of concentrating and drying, the lower temperature at 40-50 DEG C is adopted, thus ensuring that the activities of superoxide dismutase and Vc are not easily damaged.

The invention provides an anti-alcoholism beverage using tea saponin as a main body. The anti-alcoholism beverage comprises the following ingredients: tea saponin, extracts from pueraria, extracts from pericarpium citri reticulatae, jujube date polysaccharide, extracts from medlar, extracts from haw apple, honey, citric acid, vitamin B1, vitamin B6 and vitamin C. The disclosed anti-alcoholism beverage is high in raw material utilization ratio, low in cost and good in taste, and can protect liver and stomach. According to the anti-alcoholism beverage provided by the invention, the drunkenness endurance time of mice can be prolonged, the sleeping time is reduced, the concentration of ethyl alcohol in blood can be obviously reduced, the activity of in vivo glutathione peroxidase and the activity of superoxide dismutase after drinking are increased, the metabolism of the ethyl alcohol and the elimination of free radicals in the liver are accelerated, the generation of peroxides is reduced, the increase of malondialdehyde content caused by alcohol is inhibited, the activity of ethanol dehydrogenase is further increased to a certain extent, the metabolism of the ethyl alcohol in the liver is quickened, the damage of the ethyl alcohol on human bodies is reduced, and the anti-alcoholism beverage has good taste.

The invention belongs to the technical field of processing of health-care products and relates to a compound mulberry preparation capable of improving superoxide dismutase activity. The preparation method for the compound mulberry preparation comprises the following steps of: extracting 12g of mulberry and 9g of medlar in ethanol of which the concentration is 70 percent and the amount is 10 timesthat of the mulberry and the medlar at room temperature for 10 hours, sealing and crushing the extract with ultrasonic waves for 30 minutes, filtering the extract, and extracting the filtration residue with ethanol of which the concentration is 70 percent and the amount is 6 times and 4 times that of the filtration residue again for one time respectively; combining the filtrates, reclaiming the ethanol to obtain an extract of the mulberry and medlar; centrifuging 20g of porphyridium cruentum with a centrifugal for 10 minutes, suspending the porphyridium cruentum in 50mmol / L of phosphate buffer of which the pH value is 7.4, crushing the porphyridium cruentum with ultrasonic waves, centrifuging the crushed porphyridium cruentum with a high-speed centrifugal for 15 minutes to obtain a liquidsupernatant, and refrigerating and drying the liquid supernatant to obtain a dried product; and uniformly mixing the dried product and the extract of the mulberry and medlar to obtain the mulberry preparation. The mulberry preparation can be applied to a natural antioxidant which is newly developed in fields, such as food, medicament and the like, and contributes to industrial production.

The invention provides a fresh-keeping agent for cut gerbera flowers. Based on 1L, the fresh-keeping agent comprises the following effective components in part by weight: 20-30g of sucrose, 40-120mg of 8-hydroxyquinoline, 60-180mg of citric acid, 1-5mg of 6-benzyladenine. The fresh-keeping agent provided by the invention has the advantages that in order to prolong the lives of the cut flowers and improve the quality of the cut flowers, the fresh-keeping agent containing a plant-growth regulating agent is used for carrying out bottle planting test, the fresh-keeping effect of the plant-growth regulating agent is researched by analyzing all bottle-planting indexes and physiological-biochemical indexes, and the result shows that the fresh-keeping agent containing the 6-benzyladenine has the effects of prolonging the lives of the cut flowers, increasing the change rate of fresh weight of the cut flowers, improving the water balance, adding soluble sugar and protein in pedals of the cut flowers, adjusting the content of proline and the activity of superoxide dismutase and improving the permeability of cell membranes.

The invention discloses a method for quickly screening stichopus japonicus immune enhancers with high throughput, which is characterized by comprising the following steps: firstly determining beta-glucan, peptidoglycan, chitosan, mannan-oligosaccharide, CpG-oligodeoxynucleotide, soy peptide, lactoferrin, levamisole, vitamin C and vitamin E as the screened immune enhancers, then carrying out primary culture for stichopus japonicus coelom cells, detecting four sensitive immune indexes of the immune enhancers on stichopus japonicus by the cultured stichopus japonicus coelom cells, namely effects of phagotrophy rate, superoxide anion content, superoxide dismutase activity and nitric oxide synthase activity, and determining a screening result for the effects of the four indexes according to the immune enhancers. The method can screen the immune enhancers on large scale and in short time, and simultaneously can research the action mechanisms of the various immune enhancers and lay a foundation for the deep study of the immune enhancers.

A method for increasing the activity of superoxide dismutase comprises steps of: liquid culture, by providing a strain of Bacillus subtilis and a liquid medium to carry out a fed-batch fermentation and to obtain a primary fermentation broth, wherein the liquid medium has 8 wt % to 10 wt % of maltose, 10 wt % to 15 wt % of soya powder and rest of water; and solid culture, by culturing the primary fermentation broth in a solid medium, which has solid substrate and maltose in a ratio of 10:1 to 20:1, to obtain a secondary fermentation broth with SOD in high activity, wherein the solid substrate contains wheat germ and water.

The invention belongs to the traditional Chinese medicine pharmacy field, and relates to an application of leonurine SCM198 in preparation of medicines used for preventing and treating parkinson disease. According to the invention, neurotoxin 6-OHDA induces damage of nerve cells in rats, the result shows that leonurine SCM198 is capable of improving behavior changes of PD model rats, increasing the survival rate of damaged nerve cells, raising antioxidant enzyme superoxide dismutase activity, minimizing excessive generation of active oxygen species, stabilizing mitochondrial membrane potential, minimizing apoptotic cells and regulating the change level of Bax and Bcl2 gene and protein. The result proves that the leonurine SCM198 has protection effect on a PD model, and can be used for preparing medicines for preventing and treating PD.

The invention relates to a bio-enzyme preparation applied to ancient tea trees and other ancient trees. The bio-enzyme preparation is characterized by being prepared by mixing three or five of protease, superoxide dismutase activity (SOD), a chlorophyll extract, photosynthetic enzyme, polymeric carbon enzyme, sea algae extracts and urease at different mass ratios. The bio-enzyme preparation is prepared from the components which include but are not limited to the following three components in percentage by mass: 95 to 99% of protease, 2.0 to 0.6% of chlorophyll extract, and 3.0 to 0.4% of urease. The bio-enzyme preparation is prepared from the components which include but are not limited to the following five components in percentage by mass: 10 to 15% of SOD, 15 to 20% of chlorophyll extract, 10 to 15% of urease, 40 to 50% of sea algae extracts and 10 to 15% of polymeric carbon enzyme. The bio-enzyme preparation has the beneficial effects of promoting the activity of the circulating systems of the trees, improving the photosynthesis, regenerating the root system cell tissues to increase nutrition supplied to the trees, activating the activity of various enzymes of the branches, and prolonging the lifespan of various ancient trees.

The invention provides a method for extracting superoxide dismutase with a high yield from corn. The method comprises the following steps of: corn slurry-adding copper salt and separating-membrane filtering-precipitating organic liquid-drying-dissolving-conducting chromatography-freezing and drying and the like. According to the method, the copper salt is adopted to improve about 20% of activity of superoxide dismutase, and copper ion damage caused by the copper salt can be removed by means of subsequent membrane filtering as well as precipitating organic liquid for many times and drying. According to the invention, almost impurities in SOD (Superoxide Dismutase) are removed by combined steps of precipitating-drying-dissolving for multiple times by using time difference when superoxide dismutase and almost impurities (heteroprotein, nucleic acid and soluble starch) are completely dissolved in water, so that the purity is improved.

The invention relates to a method for enhancing immunity of Fenneropenaeus chinensis. The method is characterized in that Fenneropenaeus chinensis is fed with single Fenneropenaeus chinensis basal feed and Fenneropenaeus chinensis basal feed mixed with 1.4% (by weight) of hydrolyzed fish protein powder alternately, i.e. Fenneropenaeus chinensis is firstly fed with Fenneropenaeus chinensis basal feed mixed with 1.4% (by weight) of hydrolyzed fish protein powder for 7 days, and then fed with single Fenneropenaeus chinensis basal feed for 7 days, over and over again. The measured serum acid phosphatase activity, serum phenol oxidase activity, serum lysozyme activity, peroxidase activity change and serum superoxide dismutase activity of Fenneropenaeus chinensis fed by using the method provided by the invention are not significantly different from those of a Fenneropenaeus chinensis group continuously fed with hydrolyzed fish protein powder and a Fenneropenaeus chinensis group continuously fed with peptidoglycans; and the survival rate and weight increase rate are not significantly different from those of the Fenneropenaeus chinensis group continuously fed with hydrolyzed fish protein powder and the Fenneropenaeus chinensis group continuously fed with peptidoglycans. Therefore, compared with the method in which Fenneropenaeus chinensis is continuously fed with Fenneropenaeus chinensis basal feed mixed with 1.4% (by weight) of hydrolyzed fish protein powder, the method provided by the invention can obtain the same immunity effect but can reduce half of economic cost.

The invention provides a determination method for the activity of superoxide dismutase in tobacco leaves damaged by pesticides. The determination method comprises the following steps that 1, reagents are prepared, wherein the phosphate buffer, the extracting medium, the methionine solution, NBT, EDTA-Na2 and the riboflavin solution are prepared; 2, an enzyme solution is prepared from the tobacco leaves; 3, the activity of a superoxide dismutase sample is determined; 4, a tobacco is transplanted into pesticidal soil of which the quinclorac adding concentrations are 1.04*10<-3> mg / kg, 2.08*10<-3> mg / kg, 4.17*10<-3> mg / kg, 8.33*10<-3> mg / kg and 1.67*10<-2> mg / kg in the period that the tobacco has 5-6 true leaves, and the activity of the superoxide dismutase in the tobacco leaves is measured on the 7 day, the 14 day, the 21 day, the 28 day and the 35 day by adopting the step 1 to the step 3 separately. Accordingly, the problems that in the prior art, few influences that the SOD is subjected to and few changes of the SOD in the tobacco leaves damaged by quinclorac are known, and determination is not precise enough are solved.

The invention relates to an anti-radiation compound, wherein it comprises 5'-nucleotide at 10-30%, vitamin C at 5-15%,and soybean oligosaccharide at 20-40%, while its serolysin HC50 and hyperoxide dismutation enzyme activity are improved (p<0.05); and the user weight and other indexes have no significant difference (p>0.05).

The invention relates to the technical field of black garlic, and provides a black garlic processing method. The black garlic processing method comprises the following steps of 1, cleaning the fresh garlic to obtain the clean fresh garlic; 2, peeling the clean fresh garlic, remaining one to two garlic peels, dewatering, and freezing; 3, performing microwave treatment on the frozen garlic, and then naturally cooling to the room temperature; 4, performing low-frequency ultrasonic treatment on the garlic which is cooled to the room temperature after the microwave treatment. The black garlic processing method has the advantages that compared with the fresh garlic, the content of free amino acid of the black garlic is increased by 40 to 45 percent, the superoxide dismutase activity is increased by 13 to 16 times, the hydroxyl removal rate is increased by 10 to 14 times, and the polyphenol content is increased by 7 to 10 times; compared with the prior art, the quality of the black garlic is greatly increased; the black garlic has sour and sweat taste, the pungency and garlic odor can be avoided, and the mouth feel is better.