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Application of rhodium sheet nano enzyme in simulation of enzymatic catalysis

A nano-enzyme and biological enzyme technology, applied in the field of nano-enzymes, can solve the problems of single catalytic function, poor activity and insufficient catalytic efficiency, and achieve the effect of expanding the application range and widening the pH value and temperature range.

Active Publication Date: 2018-08-24
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are several outstanding problems in the current nanozyme research: (1) the catalytic efficiency of layered materials is not high enough, and the activity is relatively poor compared to HRP; (2) the materials mainly show properties similar to peroxidases, while other analogs The properties of enzymes have not been fully explored, so the catalytic function is relatively simple; (3) In a broader sense, the composition of nanozymes is mostly limited to carbon-based materials and materials containing Fe, Co, Ni, Mn, Au, Pt, Ag, Cu, Metals such as Pd, W, Mo, Ce, Ru, Ir, etc. or their alloys, oxides, sulfides, and halides, etc., while other elements such as Rh are rarely used
However, to date, single atomic layer metallo-nanozymes have not been reported

Method used

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  • Application of rhodium sheet nano enzyme in simulation of enzymatic catalysis
  • Application of rhodium sheet nano enzyme in simulation of enzymatic catalysis
  • Application of rhodium sheet nano enzyme in simulation of enzymatic catalysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Take acetic acid-sodium acetate buffer (pH=4), add the prepared rhodium flake dispersion and TMB solution, so that the concentrations of rhodium flakes and TMB in the system are respectively 3.75 μg mL -1 and 0.5mM, shake well, and leave it open at room temperature for 10 minutes; then observe the color change of the system, and scan the 325-800nm ​​ultraviolet-visible spectrum.

[0058] figure 1 The result shows that the system is colorless when the rhodium sheet dispersion is not added, and after adding the rhodium sheet dispersion, the system turns blue, and the ultraviolet-visible spectrum results are as follows: figure 2 Shown; It shows that rhodium sheet can imitate oxidase to catalyze oxygen oxidation of TMB, and has similar oxidase activity.

Embodiment 2

[0060] Take acetic acid-sodium acetate buffer solution (pH=4), add rhodium flake dispersion, TMB solution and hydrogen peroxide solution, so that the concentrations of rhodium flakes, TMB and hydrogen peroxide in the system are respectively 1 μg mL -1 , 0.5mM and 0.5mM, shake well, and place at room temperature for 10 minutes; then observe the color change of the system, and scan the 325-800nm ​​ultraviolet-visible spectrum.

[0061] figure 1 The result shows that the system is colorless when the rhodium sheet dispersion is not added, and after adding the rhodium sheet dispersion, the system turns blue; the ultraviolet-visible spectrum results are as follows figure 2 As shown, compared with the results of Example 1, the absorption value at 652nm is significantly increased, indicating that the rhodium sheet can simulate peroxidase to catalyze the oxidation of TMB by hydrogen peroxide, and has a similar peroxidase activity.

[0062] figure 1 The results also showed that rhodi...

Embodiment 3

[0064] Take PBS (pH=7), add xanthine solution, NBT solution, then add a certain amount of pre-concentrated rhodium sheet dispersion and xanthine oxidase solution, so that the concentrations of xanthine, NBT and xanthine oxidase are respectively 0.4mM , 0.4mM and 0.125U·mL -1 . In addition, a control experiment without adding rhodium plate dispersion was performed. At room temperature and in the air, when the superoxide radical produced by the xanthine / xanthine oxidase system reduces NBT, the solution turns from yellow to blue, and the maximum absorption peak appears at 560nm.

[0065] Measure the absorbance of the reaction mixture at 560nm, find out the linear relationship between the change value of the absorbance per minute and the reaction time, and determine the optimal reaction time. Finally, within the optimal reaction time, calculate the change of the absorbance of the reaction mixture before and after adding the rhodium flakes of various concentrations, and obtain th...

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Abstract

The invention provides an application of rhodium sheet nano enzyme in simulation of enzymatic catalysis. According to the application of the rhodium sheet nano enzyme in simulation of enzymatic catalysis, compared with a common rhodium catalytic hydrogenation reaction, the invention develops a catalysis application of the brand new rhodium sheet nano enzyme, the rhodium sheet nano enzyme has fourcatalytic activities of high simulation peroxidase activity, simulation oxide enzyme activity, simulation superoxide dismutase activity, and simulation catalase activity, the rhodium sheet nano enzymehas a wide pH value and a temperature usage scope, and can be adapted to various complex environment conditions of catalysis and detection, is benefit for a further development application scope, canbe used as the novel multifunctional nano enzyme for the fields of immunization analysis, biochemical detection and clinical diagnosis, and has very good market prospect and high application value.

Description

technical field [0001] The invention belongs to the field of nanozymes and relates to the application of rhodium plate nanozymes in catalysis of simulated biological enzymes. Background technique [0002] Since the advent of single-layer graphene, a series of two-dimensional graphene-like layered materials have been reported. Due to the large specific surface area, multiple active sites, adjustable catalytic activity and high stability, two-dimensional graphene-like materials have attracted extensive attention in the fields of materials, chemistry and biology as mimic enzymes. Such as few-layer metal nanosheets, transition metal sulfides, transition metal oxides, graphitic carbon nitride, layered double hydroxides, metal-organic framework materials, etc., exhibit similar horseradish peroxidase (HRP) properties, showing great application potential in the fields of biocatalysts, biosensors and biopharmaceuticals. However, there are several outstanding problems in the current...

Claims

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Application Information

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IPC IPC(8): G01N31/10
CPCG01N31/10
Inventor 杨蓉蔡双飞肖蔚王琛
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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