Preparation and application methods of penaeus monodon fermented feed

Abstract

The invention relates to preparation and application methods of penaeus monodon fermented feed. The preparation method comprises the following steps: respectively carrying out enlarged culture on bacillus licheniformis, lactobacillus plantarum and candida utilis, then mixing in a volume ratio of 1:1:1, adding brown sugar (2g / 1000mL) and running water into the mixed bacterial solution under the condition that the ratio of the culture solution to water is 1:80, uniformly mixing, uniformly stirring with penaeus monodon compound feed in a ratio of 2:5, sealing with a plastic film, and fermenting for 18-28 hours, so that the fermented feed is obtained. The invention also provides the application method of the fermented feed. The prepared fermented feed is used for feeding penaeus monodon once a day, and the feeding amount is 20-30% of the daily total feeding amount. The prepared fermented feed can obviously improve nitric oxide synthase activity, superoxide dismutase activity, catalase activity, phenoloxidase activity, glutathione peroxidase activity and total antioxidant capacity of penaeus monodon and obviously reduce the malondialdehyde content; and composition of florae in intestines of the penaeus monodon is obviously improved, and the survival rate of penaeus monodon culture is obviously increased.

Description

technical field [0001] The invention belongs to the technical field of feed processing, and in particular relates to the preparation and application method of fermented feed for Penaeus vannamei. Background technique [0002] Fermented feed is a biologically fermented feed that uses microorganisms as biological feed starter strains to convert feed materials into microbial cell protein, biologically active small peptide amino acids, and microbially active probiotics. [0003] It has been more than 30 years since the introduction of Litopenaeus vannamei to China in the late 1980s. With the advancement of science and technology, the breeding density and intensification are getting higher and higher. The output of the product has promoted the development of the aquaculture industry; on the other hand, it has also brought about a serious problem that cannot be ignored-the deterioration of the aquaculture environment, coupled with the degradation of the excellent germplasm of praw...

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Problems solved by technology

[0004] In order to control the raging diseases, antibiotics have been used in large quantities. Although the effect was obvious at first, the long-term and uncontrolled abuse of antibiotics has brought a series of problems to animals, farmed products and the environment.

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