34 results about "Porphyridium cruentum" patented technology

This invention focuses on a novel process in which Porphyridium cruentum biomass first undergoes a stage of cellular disruption and subsequently stages of recovery and purification in order to achieve the purified B-phycoerythin (BFE) protein dye, using isoelectric precipitation and two-aqueous-phase systems. The steps of recovery and purification include isoelectric precipitation followed by a step of liquid / liquid extraction by means of two-aqueous-phase systems that use polyethylene glycol (PEG) and phosphate salts. The BFE protein dye obtained in the two-aqueous-phase extraction step undergoes an ultrafiltration step in order to remove the polymer (PEG) and to obtain a dye with a purity greater than 4.0 defined as the relationship between the absorbencies at 545 and 280 nm (BFE purity=Abs 545 nm / Abs 280 nm).

The invention belongs to the technical field of processing of health-care products and relates to a compound mulberry preparation capable of improving superoxide dismutase activity. The preparation method for the compound mulberry preparation comprises the following steps of: extracting 12g of mulberry and 9g of medlar in ethanol of which the concentration is 70 percent and the amount is 10 timesthat of the mulberry and the medlar at room temperature for 10 hours, sealing and crushing the extract with ultrasonic waves for 30 minutes, filtering the extract, and extracting the filtration residue with ethanol of which the concentration is 70 percent and the amount is 6 times and 4 times that of the filtration residue again for one time respectively; combining the filtrates, reclaiming the ethanol to obtain an extract of the mulberry and medlar; centrifuging 20g of porphyridium cruentum with a centrifugal for 10 minutes, suspending the porphyridium cruentum in 50mmol / L of phosphate buffer of which the pH value is 7.4, crushing the porphyridium cruentum with ultrasonic waves, centrifuging the crushed porphyridium cruentum with a high-speed centrifugal for 15 minutes to obtain a liquidsupernatant, and refrigerating and drying the liquid supernatant to obtain a dried product; and uniformly mixing the dried product and the extract of the mulberry and medlar to obtain the mulberry preparation. The mulberry preparation can be applied to a natural antioxidant which is newly developed in fields, such as food, medicament and the like, and contributes to industrial production.

This invention focuses on a novel process in which Porphyridium cruentum biomass first undergoes a stage of cellular disruption and subsequently stages of recovery and purification in order to achieve the purified B-phycoerythin (BFE) protein dye, using isoelectric precipitation and two-aqueous-phase systems. The steps of recovery and purification include isoelectric precipitation followed by a step of liquid / liquid extraction by means of two-aqueous-phase systems that use polyethylene glycol (PEG) and phosphate salts. The BFE protein dye obtained in the two-aqueous-phase extraction step undergoes an ultrafiltration step in order to remove the polymer (PEG) and to obtain a dye with a purity greater than 4.0 defined as the relationship between the absorbencies at 545 and 280 nm (BFE purity=Abs 545 nm / Abs 280 nm).

The invention belongs to the technical field of research and development of medical products, and relates to a preparation method for a compound salvia miltiorrhiza preparation and antioxidant activity thereof. The method comprises the following steps of: adding salvia miltiorrhiza and epimedium herb into 70 percent ethanol to lixiviate at room temperature; performing ultrasonic processing for 30 minutes at the temperature of 30 DEG C and at the power of 80 W; filtering; repeatedly extracting filter residue by using 70 percent ethanol in an amount which is 6 times the amount of the filter residue and in an amount which is 4 times the amount of the filter residue respectively; filtering; combining filtrates; recovering the ethanol to obtain salvia miltiorrhiza and epimedium herb extract; centrifuging porphyridium cruentum by using a centrifuge of 5,000r / min for 10 minutes; suspending in 50mmol / L phosphoric acid buffer solution with pH of 7.4; performing ultrasonic crushing; centrifuging by using a high-speed freezing centrifuge to obtain supernatant; freeze-drying to obtain a dry product; and uniformly mixing the dry product with the salvia miltiorrhiza and epimedium herb extract to obtain the compound salvia miltiorrhiza preparation. The product has an antioxidant effect.

The invention discloses a method for harvesting porphyridium cruentum based on foam floatation. According to the method, rhamnoilpid is used in cooperation with a foaming agent, a floating product is collected in the mode of foam floatation, and finally the porphyridium cruentum is obtained. The method is suitable for treating porphyridium cruentum liquor within the range of 6.0*106 cells / L-2.0*109 cells / L as algal cell density in the porphyridium cruentum liquor, the concentration multiple of the algal cell density in the porphyridium cruentum liquor before treatment can reach 102 times, one or less hour is consumed for treatment, and the recovery rate of the porphyridium cruentum can reach 90% or above; floatation can be performed in a single-trough floating machine, in this way, investment cost is low, and industrialization is convenient.

The invention discloses an application of a compound 3,3',5,5'-tetrabromo-2,2'-biphenol in the aspect of algal inhibition. Algae removal spectrum test proves that the compound has excellent inhibitioneffect on eleven algae comprising thalassiosira pseudonana, chaetoceros mulleri, prorocentrum minimum, amphidinium carterae, nannochloropsis oculata, prochlorococcus, dunaliella salina, phaeodactylumtricornutum, chlorella vulgaris, tetraselmis subcordiformis, porphyridium cruentum, etc. The compound has great significance on treatment of red tide, algal bloom and the like natural hazard and canprevent eutrophication of water body.

The invention belongs to the technical field of the research and development of medical products, and relates to a method for preparing a compound integripetal rhodiola herb preparation and the antioxidation activity of the compound integripetal rhodiola herb preparation. The method comprises the following steps of: adding 70 percent ethanol into integripetal rhodiola herb and bupleurum, leaching at room temperature, performing ultrasound at the temperature of 30 DEG C under the condition of power of 80 W for 30 minutes, filtering, extracting filter residues once again by using 70 percent ethanol in an amount 6 times and 4 times that of the filter residues respectively, and filtering to obtain filtrate; mixing the filtrate, and recovering the ethanol to obtain a integripetal rhodiola herb and bupleurum extract; centrifuging porphyridium cruentum by using a centrifugal machine of which the rotation speed is 5,000 revolutions / minute for 10 minutes, suspending in phosphate buffer of which the concentration is 50 mmol / L and the pH value is 7.4, performing ultrasonication, centrifuging by using a high-speed freezing centrifugal machine to obtain supernate, and freezing and drying to obtain a dry substance; and mixing the dry substance and the integripetal rhodiola herb and the bupleurum extract uniformly to obtain the compound integripetal rhodiola herb preparation. The compound integripetal rhodiola herb preparation has an antioxidation effect.

Methods of use and compositions useful for moisturizing skin, conditioning skin, reducing lines and wrinkles, evening skin tone, lightening skin tone, increasing skin radiance, elasticity, skin barrier function, and / or skin firmness, reducing photodamage, reducing sagging skin, increasing anti-oxidant capacity in skin, MMP-1, MMP-3, MMP-9, pro-inflammatory cytokines (e.g., lipoxygenase, IL-6, Il-8, TNF-alpha, or VEGF), and / or elastase expression in skin, and / or increasing collagen expression in skin, elastin expression in skin, laminin expression in skin, and / or fibronectin expression in skin are disclosed herein. The composition includes a combination of one or more of Rosmarinus officianalis leaf extract, Caulerpa lentillifera extract, Morus alba fruit extract, and Porphyridium cruentum extract.

The invention belongs to the technical field of processing of health-care products and relates to a compound mulberry preparation capable of improving superoxide dismutase activity. The preparation method for the compound mulberry preparation comprises the following steps of: extracting 12g of mulberry and 9g of medlar in ethanol of which the concentration is 70 percent and the amount is 10 timesthat of the mulberry and the medlar at room temperature for 10 hours, sealing and crushing the extract with ultrasonic waves for 30 minutes, filtering the extract, and extracting the filtration residue with ethanol of which the concentration is 70 percent and the amount is 6 times and 4 times that of the filtration residue again for one time respectively; combining the filtrates, reclaiming the ethanol to obtain an extract of the mulberry and medlar; centrifuging 20g of porphyridium cruentum with a centrifugal for 10 minutes, suspending the porphyridium cruentum in 50mmol / L of phosphate buffer of which the pH value is 7.4, crushing the porphyridium cruentum with ultrasonic waves, centrifuging the crushed porphyridium cruentum with a high-speed centrifugal for 15 minutes to obtain a liquidsupernatant, and refrigerating and drying the liquid supernatant to obtain a dried product; and uniformly mixing the dried product and the extract of the mulberry and medlar to obtain the mulberry preparation. The mulberry preparation can be applied to a natural antioxidant which is newly developed in fields, such as food, medicament and the like, and contributes to industrial production.