Method for absorbing metal ions through exopolysaccharide of porphyridium cruentum

An extracellular polysaccharide and metal ion technology, which is applied in chemical instruments and methods, adsorption water/sewage treatment, special compound water treatment, etc. and other problems, to achieve the effects of low cost, high recycling rate and improved purification conditions

Inactive Publication Date: 2015-05-06
季延滨
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Porphyridium exopolysaccharide is a very large molecular weight (about 7×10 6 Da) mixed sulfate ester polysaccharides. There have been many studies on the separation and extraction of coccus and spirulina polysaccharides in China, including the optimization of factors such as material-to-liquid ratio, temperature, and time. It is not deep enough, which hinders the application and development of the polysaccharide; therefore, it is necessary to further optimize the research methods such as the separation and purification of the polysaccharide
[0006] Porphyridium is a microalgae with development prospects, but the current research is still far from industrial development and production, and there are still many problems
In the past few decades, although people have carried out a lot of work and accumulated considerable experience in the domestication, breeding and industrialization of algal species, the low production efficiency, high cost, and low product quality are still problems. The main problem facing the production of Porphyridium, how to shorten its cultivation cycle and increase the growth rate is the key issue in the application of Porphyridum, due to the high production cost, the market price of Porphyrophyllum bioactive substances is relatively high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: The Porphyridum algae from the Chinese Academy of Sciences was selected and transferred to a fresh medium every two weeks by our unit to maintain the vitality of the algae species. First of all, the culture of Porphyridium algae is carried out, and the KOCH medium is first configured. The soil leachate is prepared by the following steps: Take 0.5kg of soil, add 1000ml of distilled water to a beaker or a triangular flask, seal the bottle mouth with a ventilating plug, heat in boiling water in a water bath for 2 hours, filter under sterile conditions after cooling, and take Supernatant, sterilized distilled water was added to the supernatant to a total volume of 1000ml to obtain a soil leachate. The composition and the content of each composition were: KNO 3 : 0.75g / L, KH 2 PO 4 : 0.025g / L, MgSO 4 ·7H 2 O: 0.2g / L, ferric citrate (1%): 0.0025g, soil extract: 10g / L, seawater 500g / L, distilled water 500g / L were mixed together to prepare KOCH medium.

[0037] ...

Embodiment 2

[0041] Example 2: During cultivation, the Porphyridium algae was tested under the conditions that the light intensity was 2500Lx, the ventilation rate was 300l / h, the pH was adjusted to 7, the flask was shaken 4 times at a fixed time every day, and the frequency of shaking culture was 120 times / min. , photoperiod 18L:6D, the concentration of sodium bicarbonate is 1200mg / L; the concentration of sucrose is 1%, the ammonium sulfate of 1mmol / L is selected as the nitrogen source, and the saline solution with a mass fraction of 3%, the exopolysaccharide content of the obtained P. 52%, the amount of biomass adsorbent added is 30 grams per liter of solution, the adsorption rate of exopolysaccharides from Porphyridium algae is only 27.5% at PH=14, the adsorption rate at PH=6 is 73.4%, and the adsorption rate at PH=4 : 81.6%, the adsorption rate of PH=2 is 86.8%, and the adsorption rate can reach 94.2% when PH=1. It was measured that the desorption rate of the Porphyridium algae adsorbe...

Embodiment 3

[0042] Example 3: Porphyridium was tested under the condition that the light intensity was 3000Lx, the aeration rate was 300l / h, the pH was adjusted to 8, the photoperiod was 12L:12D, the concentration of sodium bicarbonate was 800mg / L; the concentration of glucose was 2% , choose 1mmol / L ammonium sulfate as the nitrogen source, the obtained Porphyrococcus exopolysaccharide content is 49%, the adsorption rate of Porphyrococcus exopolysaccharide is only 32.5% at PH=14, and the adsorption rate at PH=6 is 73.4% , the adsorption rate of PH=2 is 84.8%, while the adsorption rate of PH=1 can reach 93.2%. Other conditions are identical with embodiment 1. It was measured that the desorption rate of the Porphyridium sorbent in sodium carbonate was 96.8%, while the desorption rate in sodium chloride salt solution was 89.5%, and other conditions were the same as in Example 1.

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PUM

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Abstract

The invention belongs to the technical field of bio-adsorbent based treatment for metal ions in wastewater, and in particular relates to a method for absorbing metal ions through exopolysaccharide of porphyridium cruentum. The method is characterized by comprising the steps of culturing porphyridium cruentum; extracting exopolysaccharide of porphyridium cruentum; absorbing the metal ions by the exopolysaccharide of porphyridium cruentum; and desorbing the exopolysaccharide of porphyridium cruentum. The method has the beneficial effects that liquid in a liquid storing tank can be prevented from overflowing; in case of fault in a third level probe, an alarm alarms, and therefore, the material waste and fault can be effectively avoided, and as a result, the filling quality can be ensured.

Description

technical field [0001] The invention belongs to the technical field of biological adsorbents for treating metal ions in wastewater, and in particular relates to a method for adsorbing metal ions by exopolysaccharides of Porphyridum algae. Background technique [0002] With social development and technological progress, new requirements are put forward for separation materials: large adsorption capacity, fast adsorption speed, and large selective adsorption capacity, which can selectively adsorb and separate the required substances or harmful substances in the solution. It is easy to desorb and can be used repeatedly; it is cheap, durable, and has a wide range of sources; it can be used in various forms; the separation technology is simple, energy consumption is low, and there are many varieties, and different varieties can be used according to different requirements. [0003] In view of the above requirements, biosorbent materials have entered people's field of vision with t...

Claims

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Application Information

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IPC IPC(8): C02F1/28
CPCC02F1/286C02F2101/20C02F2305/00
Inventor 季延滨李涛
Owner 季延滨
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