Method for preparing compound integripetal rhodiola herb preparation and antioxidation activity of compound integripetal rhodiola herb preparation
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A Rhodiola rosea and antioxidant technology, applied in the research and development field of pharmaceutical products, can solve the problems of antioxidant toxicity and carcinogenicity
Inactive Publication Date: 2012-06-27
SHANDONG UNIV AT WEIHAI
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However, synthetic antioxidants have certain toxicity and carcinogenic effects.
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Embodiment 1
[0017] 1. a kind of preparation method of compound rhodiola rosea preparation with antioxidant effect is characterized in that comprising following process:
[0018] (1) The weight ratio of raw materials is: Rhodiola rosea 9g, Bupleurum 6g, Porphyridum 10g;
[0019] (2) Add 8 times the amount of Rhodiola rosea and Bupleurum bupleurum to extract 8 times the amount of 70% ethanol at room temperature, then seal and ultrasonicate for 30 minutes at a temperature of 30°C and a power of 80W, then filter with gauze, and filter the residue with 6 times the amount and 4 times the amount of 70% ethanol was repeatedly extracted once, filtered to obtain the filtrate;
[0020] (3) The filtrate in (2) is merged, reclaims ethanol, obtains Rhodiola Rosea, Radix Bupleurum extract;
[0021] (4) Centrifuge Porphyridium algae with a 5000r / min centrifuge for 10min, then suspend it in pH 7.4, 50mmol / L phosphate buffer, perform ultrasonic crushing, break for 5s, interval 5s, 20 times, use 10000r / min...
Embodiment 2
[0025] 1. a kind of preparation method of compound rhodiola rosea preparation with antioxidant effect is characterized in that comprising following process:
[0026] (1) The weight ratio of raw materials is: Rhodiola rosea 12g, Bupleurum 9g, Porphyridum 20g;
[0027] (2) Add 10 times the amount of Rhodiola and Bupleurum 70% ethanol to leaching at room temperature for 10 hours, then seal and sonicate for 30 minutes at a temperature of 30°C and a power of 80W, then filter with gauze, and filter the residue with 6 times the amount and 4 times the amount of 70% ethanol was repeatedly extracted once, filtered to obtain the filtrate;
[0028] (3) The filtrate in (2) is merged, reclaims ethanol, obtains Rhodiola Rosea, Radix Bupleurum extract;
[0029] (4) Centrifuge Porphyridium algae with a 5000r / min centrifuge for 10min, then suspend it in pH 7.4, 50mmol / L phosphate buffer, perform ultrasonic crushing, break for 5s, interval 5s, 20 times, use 10000r / min high speed Centrifuge in ...
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Abstract
The invention belongs to the technical field of the research and development of medical products, and relates to a method for preparing a compound integripetal rhodiola herb preparation and the antioxidation activity of the compound integripetal rhodiola herb preparation. The method comprises the following steps of: adding 70 percent ethanol into integripetal rhodiola herb and bupleurum, leaching at room temperature, performing ultrasound at the temperature of 30 DEG C under the condition of power of 80 W for 30 minutes, filtering, extracting filter residues once again by using 70 percent ethanol in an amount 6 times and 4 times that of the filter residues respectively, and filtering to obtain filtrate; mixing the filtrate, and recovering the ethanol to obtain a integripetal rhodiola herb and bupleurum extract; centrifuging porphyridium cruentum by using a centrifugal machine of which the rotation speed is 5,000 revolutions / minute for 10 minutes, suspending in phosphate buffer of which the concentration is 50 mmol / L and the pH value is 7.4, performing ultrasonication, centrifuging by using a high-speed freezing centrifugal machine to obtain supernate, and freezing and drying to obtain a dry substance; and mixing the dry substance and the integripetal rhodiola herb and the bupleurum extract uniformly to obtain the compound integripetal rhodiola herb preparation. The compound integripetal rhodiola herb preparation has an antioxidation effect.
Description
technical field [0001] The invention belongs to the technical field of research and development of pharmaceutical products. The invention relates to a preparation method of a compound rhodiola preparation and its antioxidant activity. Background technique [0002] Rhodiola rosea is a perennial herb or shrub of the Sedum family, a rare wild plant that grows in the alpine and pollution-free areas at an altitude of 800-2500 meters. Due to its harsh growth environment, such as hypoxia, low temperature and dryness, strong wind, ultraviolet radiation, and large temperature difference between day and night, it has strong vitality and special adaptability. It is a traditional medicinal material commonly used in Asia. Rhodiola is mainly used as medicine from its roots and rhizomes, and the whole plant can also be used as medicine. [Rhodiola rosea water decoction, rhodiola rosea extract and salidroside have anti-fatigue effects. Rhodiola can reduce the load on the heart, improve he...
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