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Preparation method of compound Chinese magnoliavine fruit preparation and antioxidant activity thereof

A technology of Schisandra chinensis and anti-oxidation, which is applied in the research and development field of pharmaceutical products and can solve the problems of carcinogenicity and antioxidant toxicity.

Inactive Publication Date: 2012-06-27
SHANDONG UNIV AT WEIHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, synthetic antioxidants have certain toxicity and carcinogenic effects.

Method used

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  • Preparation method of compound Chinese magnoliavine fruit preparation and antioxidant activity thereof
  • Preparation method of compound Chinese magnoliavine fruit preparation and antioxidant activity thereof

Examples

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Effect test

Embodiment 1

[0017] 1. a preparation method of compound Schisandra chinensis preparation with antioxidant effect, is characterized in that comprising following process:

[0018] (1) The weight ratio of raw materials is: Schisandra 9g, Radix Astragalus 6g, Porphyridum 10g;

[0019] (2) Add 8 times the amount of Schisandra chinensis and Astragalus membranaceus to 70% ethanol to extract 8 times at room temperature, then seal and sonicate for 30 minutes at a temperature of 30°C and a power of 80W, then filter with gauze, and use 6 times and 4 times the amount of filter residues respectively Repeat the extraction with 70% ethanol once, filter to obtain the filtrate;

[0020] (3) The filtrates in (2) are combined, ethanol is reclaimed, and Schisandra chinensis and Radix Astragali extracts are obtained;

[0021] (4) Centrifuge Porphyridium algae with a 5000r / min centrifuge for 10min, then suspend it in pH 7.4, 50mmol / L phosphate buffer, perform ultrasonic crushing, break for 5s, interval 5s, 20 ...

Embodiment 2

[0025] 1. a preparation method of compound Schisandra chinensis preparation with antioxidant effect, is characterized in that comprising following process:

[0026] (1) The weight ratio of raw materials is: Schisandra chinensis 12g, Astragalus membranaceus 9g, Porphyridum 20g;

[0027] (2) Add 10 times the amount of Schisandra chinensis and Astragalus membranaceus to 70% ethanol to extract at room temperature for 10 hours, then seal and sonicate for 30 minutes at a temperature of 30°C and a power of 80W, then filter with gauze, and use 6 times and 4 times the amount of filter residues respectively Repeat the extraction with 70% ethanol once, filter to obtain the filtrate;

[0028] (3) The filtrates in (2) are combined, ethanol is reclaimed, and Schisandra chinensis and Radix Astragali extracts are obtained;

[0029] (4) Centrifuge Porphyridium algae with a 5000r / min centrifuge for 10min, then suspend it in pH 7.4, 50mmol / L phosphate buffer, perform ultrasonic crushing, break ...

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Abstract

The invention belongs to the technical field of research and development of medical products, and relates to a preparation method for a compound Chinese magnoliavine fruit preparation and antioxidant activity thereof. The method comprises the following steps of: adding Chinese magnoliavine fruit and astragalus root into 70 percent ethanol to lixiviate at room temperature; performing ultrasonic processing for 30 minutes at the temperature of 30 DEG C and at the power of 80 W; filtering; repeatedly extracting filter residue by using 70 percent ethanol in an amount which is 6 times the amount ofthe filter residue and in an amount which is 4 times the amount of the filter residue respectively; filtering; combining filtrates; recovering the ethanol to obtain Chinese magnoliavine fruit and astragalus root extract; centrifuging porphyridium cruentum by using a centrifuge of 5,000r / min for 10 minutes; suspending in 50mmol / L phosphoric acid buffer solution with pH of 7.4; performing ultrasonic crushing; centrifuging by using a high-speed freezing centrifuge to obtain supernatant; freeze-drying to obtain a dry product; and uniformly mixing the dry product with the Chinese magnoliavine fruit and astragalus root extract to obtain the compound Chinese magnoliavine fruit preparation. The product has an antioxidant effect.

Description

technical field [0001] The invention belongs to the technical field of research and development of pharmaceutical products. The invention relates to a preparation method of a compound schisandra preparation and its antioxidant activity. Background technique [0002] Schisandra chinensis (Schisandra chinensis (Turcz.) Baill.) is a perennial deciduous vine. It is the dry mature fruit of Schisandra chinensis (Turcz.) Baill.) commonly known as "Northern Schisandra", or Schisandra sphenanthera Rehd.etWils. Jane once said: "The five flavors are divided into north and south. Those from the south are red in color, and those from the north are black in color. Human nourishing medicine must use the one from the north to be good." The 2000 edition of the Chinese Pharmacopoeia recorded the northern Schisandra and southern Schisandra as two varieties. Schisandra only refers to the dry and mature fruit of Schisandra chinensis. And Southern Schisandra specifically refers to the dry matu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/57A61P39/06
Inventor 张崇禧张成城侯欣池郑友兰
Owner SHANDONG UNIV AT WEIHAI
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