Method for testing typical antibiotics wastewater toxicity by using zebra fishes

A technology of antibiotic wastewater and testing method, applied in the field of environmental toxicology, can solve problems such as the impact of higher organisms that cannot represent antibiotic wastewater

Inactive Publication Date: 2014-03-05
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Problems solved by technology

The emission indicators related to toxicity are only acute toxicity indicators (HgCl 2 toxicity equivalent), the test method is luminescent bacteria method, this index can only reflect whether antibiotic wastewater is toxic to luminescent bacteria, but luminescent bacteria are lower organisms, and the results cannot represent the impact of antibiotic wastewater on higher organisms, so it is necessary to find new Methods and indicators to evaluate the biotoxicity of antibiotic wastewater

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  • Method for testing typical antibiotics wastewater toxicity by using zebra fishes
  • Method for testing typical antibiotics wastewater toxicity by using zebra fishes
  • Method for testing typical antibiotics wastewater toxicity by using zebra fishes

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example 1

[0038] Hereinafter, taking penicillin wastewater as an example, the specific implementation of the toxicity testing method proposed by the present invention will be described.

[0039] The juvenile zebrafish (Zebra Fish) was selected as the test organism. The zebrafish was provided by the Experimental Animal Center of Hebei Medical University, with a body length of (34.05±0.43) mm and a body weight of (0.24±0.03) g. The fish used in the experiment were sterilized by 5% saline and then entered into the laboratory. They were bred in the laboratory for 7 days with dechlorinated tap water aerated for 48 hours. They were not fed the day before and during the experiment to avoid the influence of bait on the experiment. The test conditions meet the requirements.

[0040] (1) Pre-test method Put 20L of test solution in a 50cm×20cm×25cm glass aquarium. The test solution is a mixture of penicillin wastewater and dechlorinated tap water.

[0041] The pre-test set 6 exposure groups, the...

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Abstract

The invention belongs to the field of environmental toxicology, and relates to a method for testing typical antibiotics wastewater toxicity by using zebra fishes. According to the method, zebra fishes are used as to-be-tested organisms, and are exposed in typical antibiotics wastewater, the short-term or long-term toxic effect of the typical antibiotics wastewater to the zebra fishes is quantitatively tested through analyzing the acute toxicity and subacute toxicity of the typical antibiotics wastewater to the zebra fishes, the acute toxicity is described by using the 96h medium lethal concentration (96h LC50) (typical antibiotics wastewater percent by volume percent), and the subacute toxicity is described by fish body biochemical indexes such as superoxide dismutase (SOD) activity, peroxidase (POD) activity and malondialdehyde (MDA) content. By adopting the method, the toxicity characteristics and toxicity levels of the typical antibiotics wastewater can be analyzed, tested and quantitatively described, and the problem that conventional physicochemical indexes can not reflect biotoxicity of the typical antibiotics wastewater can be solved, and results obtained by analyzing, testing and describing can be used as biotoxicity monitoring and evaluating indexes of the typical antibiotics wastewater.

Description

technical field [0001] The invention belongs to the field of environmental toxicology and relates to a method for testing the toxicity of typical antibiotic wastewater by using zebrafish. Background technique [0002] Antibiotic wastewater is a kind of high-concentration organic wastewater with high chroma, refractory and biotoxic substances. In July 2010, the Ministry of Environmental Protection promulgated the "Pharmaceutical Industry Water Pollutant Discharge Standard", which stipulates the discharge limits, monitoring and monitoring requirements of water pollutants in fermented pharmaceutical industrial enterprises, mainly antibiotics. The emission indicators related to toxicity are only acute toxicity indicators (HgCl 2 toxicity equivalent), the test method is luminescent bacteria method, this index can only reflect whether antibiotic wastewater is toxic to luminescent bacteria, but luminescent bacteria are lower organisms, and the results cannot represent the impact o...

Claims

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Application Information

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IPC IPC(8): G01N33/18
Inventor 沈洪艳杨雷武晨虹
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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