The invention discloses an antibacterial wet tissue and a preparation method thereof, and relates to the technical field of wet tissue preparation. The antibacterial wet tissue comprises a wet tissue body and wet tissue liquid, wherein the wet tissue liquid comprises the following components: deionized water, a surfactant, vitamin E, an aloe extracting solution, citric acid, glycerol, a solubilizer, borneol, water-soluble chitosan, ethyl p-hydroxybenzoate, chlorhexidine acetate, a traditional Chinese medicine extracting solution and a humectant. According to the traditional Chinese medicine extracting solution, radix sophorae flavescentis, radix glycyrrhizae, fructus cnidii, scutellaria baicalensis, folium isatidis, herba houttuyniae, pericarpium citri reticulatae and fructus forsythiae are combined for use, the synergistic and antibacterial effects are achieved under the condition that the multiple components are compounded, the effect of the components is obviously better than that of independently added one component, or every two components cooperate to achieve the effects of clearing heat, drying dampness, eliminating turbid pathogen with aromatics and preventing microbial infection, and the antibacterial wet tissue has cleaning, comfortable and health-care effects during use, does not have any irritation or side effect on a skin membrane, smells fragrant, is convenient to carry, and has an effect superior to that in the prior art.