A kind of algae polysaccharide composition and its preparation method and application
A technology of algae polysaccharides and compositions, which is applied in the field of algae polysaccharide compositions and its preparation, can solve problems such as increased disease susceptibility, decreased disease resistance, and harm to human health, so as to improve NO secretion ability, increase secretion ability, improve The effect of immune activity
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Embodiment 1
[0054] The embodiment of the present application provides a preparation method of Ulva polysaccharide, comprising the following steps:
[0055] Soak the Ulva in appropriate amount of water for 30 minutes, rinse with clean water 3 times.
[0056] Mix clean Ulva and deionized water, heat and boil for extraction for 6 hours, wherein the mass ratio of Ulva to water is 1:10, filter through a sieve to remove Ulva residue, collect the filtrate, and centrifuge the filtrate at high speed to remove impurities to obtain an extract , concentrate it to a smaller volume by ultrafiltration to obtain a concentrated solution, carry out alcohol precipitation treatment according to the volume ratio of the concentrated solution: absolute ethanol as 1:5, collect the precipitate after standing for 30 minutes, and obtain Ulva polysaccharide by vacuum freeze-drying .
Embodiment 2
[0058] The embodiment of the present application provides a preparation method of laminarin, comprising the following steps:
[0059] Soak the kelp for 20 minutes and rinse with water 3 times.
[0060] Mix the clean kelp with sodium bicarbonate solution and heat extraction, wherein the concentration of sodium bicarbonate solution is 1‰, the condition of heating extraction is 90°C for 4 hours, the mass ratio of kelp: sodium bicarbonate solution is 1:15, through Sieve and filter to remove kelp residues, collect the filtrate, and concentrate the filtrate to a smaller volume by ultrafiltration to obtain a concentrated solution, carry out alcohol precipitation treatment according to the volume ratio of the concentrated solution: absolute ethanol as 1:4, and collect the precipitate after standing for 30 minutes. Laminarin dried by vacuum freeze drying.
Embodiment 3
[0062] The embodiment of the present application provides a preparation method of bull algae polysaccharide, comprising the following steps:
[0063] Cut bull algae into pieces, soak them in water for 20 minutes, and decolorize them with 70% alcohol for 24 hours. The algae flakes are collected by filtration, rinsed with clean water, and the pretreated bull algae is obtained.
[0064] The pretreated bull algae and sodium bicarbonate solution are mixed and heated for extraction, the concentration of the sodium bicarbonate solution is 1%, and the conditions for heating and extraction are extracted at 95° C. for 4 hours, and the pretreated bull algae: the mass ratio of the sodium bicarbonate solution is 1: 20. Remove the bull algae residue by sieve and filter, collect the filtrate, concentrate it to a smaller volume by ultrafiltration, and obtain a concentrated solution, carry out alcohol precipitation treatment according to the volume ratio of the concentrated solution: 70% ethan...
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