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Method for promoting porphyridium growth by virtue of aquatic product processing wastewater

A technology for processing wastewater and Porphyridium, applied in the direction of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc. Large-scale cultivation and other issues, to achieve the effect of short cycle, shortened growth cycle, and low cost

Inactive Publication Date: 2016-07-27
FOSHAN JUCHENG BIOCHEM TECH RES & DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Porhyridium (Porhyridium) is a freshwater algae plant of the genus Porphyridium in the Porphyridaceae Porphyridae of the Rhodophyta Protoerythrophyta Porphyridium. Saline, rich in phycoerythrin, polyunsaturated fatty acids and sulfated polysaccharides and other nutrients, the maximum development and extraction of active substances in Porphyridium algae has been a research hotspot, but due to the understanding of the optimal growth conditions of Porphyrococcus Insufficient, easy to contaminate miscellaneous bacteria in the natural environment, and often suppressed by cyanobacteria, especially Oscillatoria species, which limits the large-scale cultivation of Porphyridium algae

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Precipitate the aquatic product processing wastewater, remove the precipitation, and filter the supernatant fluid twice at a speed of 0.8m / s and apply 15mJ / cm 2 Ultraviolet light, add microbial starter (China Common Microbial Culture Collection and Management Center) Lactococcus lactis 0.3kg / L, Alcaligenes 0.2kg / L fermented for 2.5 days, put in Porphyridium seeds, cultivate for 3.5 days, and measure the growth rate OD 604nm (Spectrophotometer, 723 manual, Shanghai Binglin Electronic Technology Co., Ltd.), the results are shown in Table 1. During the cultivation period, the 30-minute music "Be with you through the long years" is played once.

Embodiment 2

[0024] Precipitate the aquatic product processing wastewater, remove the precipitation, and filter the supernatant fluidly at a speed of 1.7m / s for 3 times and apply 22.5mJ / cm 2 Under ultraviolet light, add 0.55kg / L of microbial starter Lactococcus lactis, 0.4kg / L of alkali-producing bacteria, ferment for 3.25d, put in Porphyridium seeds, cultivate for 4.5d, and measure its growth rate, namely OD 604nm The results are shown in Table 1. During the cultivation period, the 40-minute music "Imprint of Rain" was played twice.

Embodiment 3

[0026] Precipitate the aquatic product processing wastewater, remove the precipitation, and filter the supernatant 4 times at a speed of 2.6m / s and apply 30mJ / cm 2 UV light, add 0.8kg / L of microbial starter Lactococcus lactis, 0.6kg / L of alkali-producing bacteria, ferment for 4 days, put in Porphyridium seeds, cultivate for 5.5 days, and measure the growth rate (OD) 604nm , The results are shown in Table 1. During the cultivation period, the 50-minute music "The Meaning of Travel" was played three times during the day.

[0027] Table 1 Porphyridium growth rate

[0028] Sample

[0029] It can be seen from Table 1 that the cell density of Porphyridium spp. cultivated by the method provided by the present invention for accelerating the growth of Porphyridium spp. on the 3.5th day of cultivation reached 1.75 and entered the stable period of growth. It can be seen that the use of aquatic products provided by the present invention The method of processing wastewater to accelerate the ...

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Abstract

The invention discloses a method for promoting porphyridium growth by virtue of aquatic product processing wastewater. The method for promoting porphyridium growth by virtue of the aquatic product processing wastewater comprises the following steps: precipitating the aquatic product processing wastewater; removing precipitate; filtering a supernatant which flows in a circulating mode and applying ultraviolet radiation; fermenting the processed supernatant with the addition of a microbial fermenting agent and adding porphyridium seeds; and breeding, and playing music during breeding, wherein the microbial fermenting agent includes lactococcus and alcaligenes. Compared with the prior art, the method disclosed by the invention has the beneficial effects that the method, which makes full use of the aquatic product processing wastewater, can reduce environmental pollution; and the method is low in cost, short in cycle, capable of shortening a porphyridium growth cycle and achieving large-scale breeding, and the method can bring about huge economic benefits.

Description

Technical field [0001] The invention belongs to the field of microalgae cultivation, and specifically relates to a method for accelerating the growth of Porphyridium sp. using aquatic product processing wastewater. Background technique [0002] Aquatic product processing wastewater is mainly the wastewater generated during the thawing and cleaning of raw materials during the processing of aquatic products. In recent years, as people’s demand for aquatic products has increased, a large amount of aquatic product processing wastewater has been directly discharged. Because the aquatic product processing wastewater contains high organic matter, grease, nitrogen and phosphorus, direct discharge will increase the eutrophication of the receiving water body. Deteriorating water quality, even causing bacterial infections, endangering humans, livestock, etc. and fishery activities, so purification and reuse of aquatic product processing wastewater is an urgent problem to be solved. [0003] ...

Claims

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Application Information

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IPC IPC(8): C12N1/12C02F9/14C12R1/89
CPCC12N1/12C02F1/32C02F1/52C02F3/347C02F9/00
Inventor 杨广群曹丽萍
Owner FOSHAN JUCHENG BIOCHEM TECH RES & DEV CO LTD
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