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Method for increasing content of phycoerythrobilin in porphyridium

A technology of phycoerythrin and Porphyridium, which is applied in the field of increasing the content of phycoerythrin in Porphyrophyllum, can solve the problems of slow growth of Porphyrophyllum and low yield of phycoerythrin, so as to improve photosynthesis and avoid massive Death, the effect of increasing production

Active Publication Date: 2019-04-23
杭州园泰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the defects of slow growth and low yield of phycoerythrin in the prior art, and provide a method for increasing the content of phycoerythrin in Porphyrophyllum

Method used

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  • Method for increasing content of phycoerythrobilin in porphyridium
  • Method for increasing content of phycoerythrobilin in porphyridium

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Embodiment 1

[0027] The method for improving the content of phycoerythrin in Porphylococcus comprises the steps of:

[0028] Inoculate the Porphyridium algae cultivated to the logarithmic growth phase into the seed tank containing the f / 2 medium, and the inoculation initial density is 2×10 5 cells / ml, light intensity 4000lux, culture at 23°C, light-to-dark ratio of 14:10, air flow rate of 0.4vvm, culture for 2 days, and collect Porphyridium algae seed liquid;

[0029] Put the Porphyridium algae seed solution into the reaction tank containing artificially synthesized culture solution according to the inoculation amount of 10%, control the light intensity to 5000Lux, the air flow rate to 0.5vvm, cultivate at 25°C, and the light-to-dark ratio is 14:10 , cultivated for 3 days, then added 0.02 mg / L of brassinolide and 0.3 g / L of bionitrogen, and continued to cultivate for 5 days to obtain Porphyrococcus algae liquid;

[0030] The components of the culture solution are: sodium chloride 12g / L, s...

Embodiment 2

[0032] The method for improving the content of phycoerythrin in Porphylococcus comprises the steps of:

[0033] Inoculate the Porphyridium algae cultivated to the logarithmic growth phase into the seed tank containing the f / 2 medium, and the inoculation initial density is 2×10 5 cells / ml, light intensity 4000lux, culture at 23°C, light-to-dark ratio of 14:10, air flow rate of 0.4vvm, culture for 2 days, and collect Porphyridium algae seed liquid;

[0034] Put the Porphyridium algae seed liquid into the reaction tank containing artificially synthesized culture liquid according to the inoculum amount of 10%, control the light intensity to 6000Lux, the air flow rate to 0.3vvm, cultivate at 22°C, and the light-to-dark ratio is 14:10 , cultivated for 3 days, then added 0.01 mg / L of brassinolide and 0.4 g / L of bionitrogen, and continued to cultivate for 4 days to obtain Porphylococcus algae liquid;

[0035] The components of the culture solution are: sodium chloride 12g / L, sodium n...

Embodiment 3

[0037] The method for improving the content of phycoerythrin in Porphylococcus comprises the steps of:

[0038] Inoculate the Porphyridium algae cultivated to the logarithmic growth phase into the seed tank containing the f / 2 medium, and the inoculation initial density is 2×10 5 cells / ml, light intensity 4000lux, culture at 23°C, light-to-dark ratio of 14:10, air flow rate of 0.4vvm, culture for 2 days, and collect Porphyridium algae seed liquid;

[0039] Put the Porphyridium algae seed solution into the reaction tank containing artificially synthesized culture solution according to the inoculum amount of 10%, control the light intensity to 6000Lux, the air flow rate to 0.5vvm, cultivate at 24°C, and the light-to-dark ratio is 14:10 , cultivated for 3 days, then added 0.04 mg / L of brassinolide and 0.34 g / L of bionitrogen, and continued to cultivate for 5 days to obtain Porphylococcus algae liquid;

[0040] The components of the culture solution are: sodium chloride 12g / L, sod...

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Abstract

The invention belongs to the technical field of algae culture, and discloses a method for increasing the content of phycoerythrobilin in porphyridium. The method comprises the following steps that a porphyridium seed solution is inoculated into a reaction tank containing an artificial synthesis culture solution, the illumination intensity is controlled to be 4000-6000 Lux, the flow of introduced air is 0.3-0.5 vvm, culture is carried out at 22-25 DEG C, the light-dark ratio is 14:10, and culture is carried out for 3 d; then brassinolide and bionitrogen are added, the mixture is continuously cultured for 3-5 d, and the porphyridium is collected. The method increases the growth rate of the porphyridium and the content of phycoerythrin.

Description

technical field [0001] The invention belongs to the technical field of algae cultivation, and in particular relates to a method for increasing the content of phycoerythrin in Porphyrococcus. Background technique [0002] Phycoerythrin (phycoerythrin) is a pigment protein present in red algae, cyanobacteria, and cryptodininosaurs. The ring-opened tetrapyrrole phycoerythrin is covalently bonded to the protein as a pigment part. The prosthetic group is a chain composed of pyrrole rings, the molecule does not contain metal, and is combined with the protein. Phycoerythrin has strong absorption in the 480-570 band in the visible light region, and can produce strong fluorescence. No matter in terms of phycoerythrin, phycocyanin or other natural protein pigments, they are usually practical and safe, and have a considerable degree of stability to heat, pH, etc., and can be used in food and cosmetics, and can also be used as an immunological It is used as a fluorescent dye for antib...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C07K14/795C12R1/89
CPCC12N1/12C07K14/795Y02A40/80
Inventor 胡叶飞张菊萍董国强张宏杰金荣培
Owner 杭州园泰生物科技有限公司
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