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81 results about "Strigolactone" patented technology

Strigolactones are a group of chemical compounds produced by a plant's roots. Due to their mechanism of action, these molecules have been classified as plant hormones or phytohormones. So far, strigolactones have been identified to be responsible for three different physiological processes: First, they promote the germination of parasitic organisms that grow in the host plant's roots, such as Striga lutea and other plants of the genus Striga. Second, strigolactones are fundamental for the recognition of the plant by symbiotic fungi, especially arbuscular mycorrhizal fungi, because they establish a mutualistic association with these plants, and provide phosphate and other soil nutrients. Third, strigolactones have been identified as branching inhibition hormones in plants; when present, these compounds prevent excess bud growing in stem terminals, stopping the branching mechanism in plants.

Tomato cutting water planting rooting seedling-raising method

The invention discloses a method for inhibiting growth and development of tomato side branches. The method comprises the following steps of performing exogenous coating to a tomato plant by adopting a Strigolactones solution with the concentration of 7.5-10.0umol*L-1; or performing exogenous injection to the tomato plant by adopting a Strigolactones solution with the concentration of 2.5-5.0umol*L-1; or soaking tomato seedling roots for 20-24 hours by adopting a Strigolactones solution with the concentration of 10-20.0umol*L-1 in 1-2 days before planting the seedlings. Exogenous coating and injection are executed at the leaf axilla of the tomato stem branch part, and the dosage thereof is 10uL / strain. According to the method, growth and development of tomato side branches can be obviously inhibited, labor consumption, and disease and pest spread are reduced, thereby improving plant growth and yield and quality; the method is simple in operation and pollution-free, and is applied to cultivation of an open field tomato and a greenhouse tomato.
Owner:HEBEI AGRICULTURAL UNIV.

Total synthesis method of strigolactones GR24

The invention discloses a novel total synthesis method of strigolactones GR24 which is a plant hormone. The total synthesis method of the strigolactones GR24 comprises the following steps of: carrying out condensation, reduction, lactonization and condensation salt forming reaction on 1-indanone to synthesize a compound F; carrying out dehydrogenation reaction and bromination on 2-methyl butyrolactone to synthesize a compound H; and finally carrying out a substitution reaction on the compound F and the compound H to obtain the strigolactones GR24. According to the total synthesis method, raw materials are low in cost and easily available, the operation is simple and feasible, no strict water-free and oxygen-free operation is required, all steps are simple and feasible except the last step which needs column chromatography isolation, and the whole synthesis route is very suitable for large-scale production. Furthermore, the total synthesis method can be universally used for synthesis of various strigolactones derivatives.
Owner:NANJING AGRICULTURAL UNIVERSITY

Method for improving tea seedling field fungus rooting cuttage breeding effects

The invention discloses a method for improving tea seedling field fungus rooting cuttage breeding effects. According to the method, AMF (arbuscular mycorrhizal fungi) efficient strain rhizophagus intraradices is applied into a cutting seedbed in advance, and strigolactones is sprayed after cutting is completed, so that the tea seedling field fungus rooting cuttage breeding effects are improved. Test results show that tea seedlings are bred by the method, the tea seedling field fungus rooting cuttage breeding effects can be effectively improved, infection rate of AMF for the tea seedlings is increased, the germination rate, the callus occurrence rate and the survival rate of the sea seedlings are increased, growth indexes such as stem height, root length and dry and fresh weight of the teaseedlings are facilitated, and the method has the advantages that the method is simple to operate, low in labor intensity and suitable for popularization and application in actual production, investment increase is less and the like.
Owner:GUIZHOU TEA RES INST

Drought-enduring seed coating agent of oilseed rape

ActiveCN108849897APromote germinationImprove germination drought resistanceBiocidePlant growth regulatorsPotassiumDodecylsulfonic acid
The invention discloses a drought-enduring seed coating agent of oilseed rape. The drought-enduring seed coating agent of the oilseed rape is prepared from the following raw materials in percentage bymass: 20.0-30.0% of ammonium polyacrylate, 6.0-10.0% of potassium acrylate, 0.05-0.5% of abscisic acid (ABA), 0.05-0.5% of strigolactone, 1.0-5.0% of chitosan, 3.0-5.0% of trehalose, 0.5-1.5% of cross-linking agent (vulcanizing agent S-80), 3.0-5.0% of sodium dodecyl sulfate, 10.0-20.0% of starch and 22.5-56.4% of distilled water. The coating agent is reasonable in formula and convenient to use and can improve the drought resistance of a whole growth period of the oilseed rape furthest; meanwhile, due to the fact that an induction factor special for the oilseed rape adversities resistance isadded into a preparation, the resistance of the oilseed rape to drought stress can be effectively induced, expression of corresponding drought resistance genes is activated, and the drought resistanceof the whole growth period of the oilseed rape is promoted; therefore, compared with ordinary seed drought-enduring coating agents, the preparation has the advantages that action is direct, the yieldimprovement effect is significant, an economic coefficient is high, the effect is durable, and the like.
Owner:INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI

Method for reducing content of H2O2 and MDA in non-heading Chinese cabbages after low-temperature stress

The invention discloses a method for reducing the content of H2O2 and MDA in non-heading Chinese cabbages after low-temperature stress, and relates to the technical field of genetic breeding. A mixedsolution (containing 0.1% Tween 80) of brassinolide (BRs) of 0.1 [mu]mol.L<-1> and strigolactone (SL) of 0.1 [mu]mol.L<-1> is sprayed. The contrast is clear water spraying and BRs and and SL spraying.The concentrations of BRs and SL are the same. According to all treatment, spraying is respectively carried out at 8: 00 and 20: 00 two days before the low temperature, and the spraying parts are theback surfaces of leaves, and spraying is carried out until the surfaces of the leaves drip water. Spraying is performed for four times in total. The mixed solution provided by the invention can effectively improve the low-temperature stress resistance of non-heading Chinese cabbage seedlings, and provides an effective guidance thought for improving the quality and yield of the non-heading Chinesecabbages after low-temperature stress.
Owner:ANHUI AGRICULTURAL UNIVERSITY +1

Method for promoting single-needle algae accumulated grease by nitrogen deficiency combined with strigolactones

The invention discloses a method for promoting single-needle algae accumulated grease by nitrogen deficiency combined with strigolactones. Single-needle algae is cultivated through two stages. The method for promoting the single-needle algae accumulated grease by the nitrogen deficiency combined with the strigolactones comprises the following steps that firstly glucose is used as an organic carbonsource to heterotrophically cultivate the single-needle algae to a logarithmic phase later period to collect algae cells; then autotrophy anazotic BG-11 is used as an inducing culture medium of a second stage to renewedly suspend the algae cells to be used as inducing algae liquid; 1mmol/L strigolactones (SLs) mother solutions are prepared by acetone, the strigolactones (SLs) mother solutions areadded into the inducing algae liquid to dilute the strigolactones (SLs) mother solutions to the concentration of 1[mu]mol/L, and the algae liquid is placed in a shaking table for lighting cultivating; and the algae cells cultivated to a stable phase are enriched by a centrifugal separation method, grease is extracted, and methyl esterification is carried out to prepare biodiesel. The method for promoting the single-needle algae accumulated grease by the nitrogen deficiency combined with the strigolactones is simple and practicable, can greatly shorten the cultivated time of the oil-producingsingle-needle algae, obviously increases the grease yield, and thus, greatly improves the efficiency of microalgae for producing the biodiesel.
Owner:KUNMING UNIV OF SCI & TECH

Full-automatic online SPE-LC-MS/MS quantitative analysis method of endogenous strigolactone in plant sample

ActiveCN109374765AAccurate Absolute Quantitative AnalysisImprove throughputComponent separationOnline speStrigolactone
The invention discloses a full-automatic online SPE-LC-MS / MS quantitative analysis method of an endogenous strigolactone in plant sample. The method comprises the following steps: (1) pretreating a plant sample to obtain a treated sample; (2) dissolving the treated sample obtained in the step (1) with a redissolving solvent to obtain a solving liquid; and (3) detecting the solving liquid in an online SPE-LC-MS / MS system, recording the strigolactone and the peak area corresponding to the stable isotope interior label of the strigolactone, and calculating according to an internal standard methodto obtain the content of endogenous strigolactone in a plant sample. The method can perform accurate absolute quantitative analysis on the endogenous strigolactone of plants, is simple and convenient, has good selectivity, high accuracy and high sensitivity, and has an important meaning to the physiological function and molecular action mechanism of strigolactone compounds.
Owner:INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI

Special synergist for beauveria bassiana biopesticide as well as preparation method thereof and application

The invention discloses a special synergist for beauveria bassiana biopesticide and a preparation method thereof, the special synergist is prepared from the following components in percentage by mass:0.05-0.5% parts of strigolactones, 1-5% parts of methanol, 5-15% parts of surfactant, 1-5% parts of filmogen, 0.5-5% parts of thickener, 3% parts of glucose, 0.5% parts of monopotassium phosphate, 0.2% parts of magnesium sulfate, 0.2% parts of calcium nitrate, and the balance of water. dissolving the filmogen, the thickener, glucose, monopotassium phosphate, magnesium sulfate and calcium nitratein water to prepare mixed liquor A; dissolving strigolactones in methanol and introducing the mixed liquid A at a stirring state, then adding a surfactant combination, after stirring, carrying out high-speed shearing for 30 min, then removing residue to obtain a finished product, the application method is to prepare the beauveria bassiana biopesticide into liquid pesticide to be applied accordingto the specification, then adding the product provided by the invention, applying the special synergist after mixing uniformly, the special synergist can significantly improve the prevention and control effect of the beauveria bassiana biopesticide, enhance the fast-acting property is enhanced and the period of preserving effect is extended.
Owner:PLANT PROTECTION & QUALITY & SAFETY OF AGRI PRODS INST ANHUI ACAD OF AGRI SCI

Multi-effect pesticide composition and application thereof

The invention relates to the technical field of pesticides, in particular to a multi-effect pesticide composition, which is prepared from the following components in parts by weight: 0.001-1 parts ofbrassinolide, 1-25 parts of mepiquat chloride, 2.5-30 parts of paclobutrazol, 1-3 parts of strigolactone, 1-5 parts of a corn stigma extracting solution and 1-5 parts of liquorice root extract. The invention also relates to application of the multi-effect pesticide composition in a cotton or peanut planting process. The application can be used for solving the problems of cotton flower falling, budfalling and boll falling and promoting bud falling regeneration. The problems of vigorous growth and lodging of the overground parts of the peanuts can be effectively controlled, the maturing rate and the grain plumpness rate of the peanuts are increased, a good yield increasing effect is achieved, and the influence of residues of paclobutrazol in soil on next-stubble crops is reduced.
Owner:江西鑫邦生化有限公司

Use of strigolactones and strigolactone analogs for treating proliferative conditions

Compound of formula X wherein P1 is a fused-ring system comprising one 6-membered and two 5-membered rings; v indicates an S or R configuration; or individual isomers or pharmaceutically acceptable salts thereof, or mixtures thereof, in the preparation of an active agent for preventing or inhibiting cell proliferation or for inducing cell death.
Owner:THE STATE OF ISRAEL MINIST OF AGRI & RURAL DEV AGRI RES ORG ARO VOLCANI CENT +1

Ornithogalum thyrsoides dwarf multi-tiller OtDWARF53 gene and application thereof

InactiveCN111118028AFull-bodiedPromote multiple tillers and multiple branchesPlant peptidesFermentationBiotechnologyAxillary bud
The invention relates to the technical field of plant genetic engineering, and in particular relates to an ornithogalum thyrsoides dwarf multi-tiller OtDWARF53 gene and an application thereof. The nucleotide sequence of the gene is shown in SEO ID NO.1, and the amino acid sequence of a protein encoded by the gene is shown in SEO ID NO.2. The key gene DWARF53 (DWF53) for regulating strigolactones is isolated from ornithogalum thyrsoides based on the obtained invention patent of induction and tissue culture of foliar bulbiets of the ornithogalum thyrsoides and based on ornithogalum thyrsoides tertiary and secondary generation transcriptome conjoint analysis results (3+2), and a constitutive plant expression vector pHB+ OtDWARF53 is constructed, the pHB+ OtDWARF53 is transferred into tobacco,results find that transgenic tobacco plants show the following phenotys: plants have dwarf phenomena, multiple shoots are generated at stem tips, axillary bud lateral buds are formed at leaf axils, and the appearance is more plump, and the results indicate that the gene can have the functions of making the plants dwarf and promoting multi-tiller and multi-branch; and the invention specifically relates to the application of the gene in plant type regulation of regulating plant heights and promoting the development of the axillary bud lateral buds.
Owner:SICHUAN TIANYI ECOLOGICAL GARDEN GRP CO LTD +2

Chrysanthemum strigolactone synthetase gene CmCCD8 and application thereof in changing chrysanthemum flowering phase

InactiveCN107502604AFlowering time changesGenetic engineeringFermentationBiotechnologyStrigolactone
The invention belongs to the field of genetic engineering and transgenic breeding, and discloses a chrysanthemum strigolactone synthetase gene CmCCD8 and application thereof in changing a chrysanthemum flowering phase. The method for controlling the chrysanthemum flowering phase by virtue of a transgene CmCCD8 comprises the following steps: cloning a CmCCD8 gene in chrysanthemum morifolium 'Jinba';establishing a pBIG-CmCCD8 plant expression vector and transferring into a chrysanthemum leaf disc, and performing the subculture to primarily obtain a transgenic plant. The observation on a phenotype of the flowering phase of the transgenic plant discovers that compared with the non-transgenic plant, the transgenic plant can bloom normally under the sunlight for a long time. By transforming the endogenesis CmCCD8 gene, the flowering time of the chrysanthemum morifolium is controlled, and a novel and practical method can be provided for improving the ornamental quality of the chrysanthemum morifolium by utilizing the genetic engineering technology, so that the breeding process of the chrysanthemum biotechnology can be effectively promoted.
Owner:NANJING AGRICULTURAL UNIVERSITY

Isotopically labeled methyl furanone, intermediate and preparation method of isotopically labeled methyl furanone

The invention discloses isotopically labeled methyl furanone, an intermediate and a preparation method of isotopically labeled methyl furanone. The invention provides isotopically labeled methyl furanone 6. The invention further provides a preparation method of isotopically labeled methyl furanone 6, and the preparation method comprises the following steps: performing removal of a hydroxy protecting group and isomerization reaction on a compound 20 in the presence of an acid. The method provided by the invention comprises short reaction steps, labeling loci are stable, are labeled on a common D ring of a strigolactone type compound family and are successfully butted with ABC rings of strigolactone type compounds to obtain a variety of isotopically labeled strigolactone type compounds with different isotopic abundances which are more than 99% respectively, and the isotopically labeled methyl furanone is applicable to wide substances, is used as an internal source standard matter for GC-MS and LC-MS / MS analysis and has high detection sensitivity and good accuracy, thereby having broad market application prospects.
Owner:SHANGHAI INST OF ORGANIC CHEMISTRY - CHINESE ACAD OF SCI

Method of screening mutants being insensitive to strigolactone

The invention discloses to a method of screening mutants being insensitive to strigolactone. The method includes the steps of: 1) processing of wile-type arabidopsis thaliana seeds: A) weighing seeds and washing the seeds in distilled water; B) adding ethylmethane sulfonate and uniformly mixing the components in a fume cupboard; C) pouring processed ethylmethane sulfonate in NaOH and cleaning the seeds; D) placing the cleaned seeds in an agar solution and moving the seeds into soil by means of a pipette gun for growth; 2) screening of the mutants being insensitive to strigolactone: A) when the seeds are matured, harvesting the seeds and placing the seeds in a centrifugal tube, and disinfecting the seeds with a bleaching agent solution and placing the seeds in a refrigerator; B) sowing the seeds on an AT culture medium containing the strigolactone by means of the pipette gun; C) irradiating the culture dish, and placing the culture dish in a dark place so that the seeds are germinated; and D) selecting a long-root-system arabidopsis thaliana mutant single plant and transplanting the single plant in soil. The method has simple operations and short screening period, and can quickly and high-effectively screen the arabidopsis thaliana mutants being insensitive to the strigolactone.
Owner:INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI

Method for promoting germination of cynomorium songaricum seed by sodium chloride and GR24

The invention discloses a method for promoting the germination of cynomorium seeds by using sodium chloride and GR24. The method adopts a reagent composed of sodium chloride and strigolactone artificially synthesized analogue GR24 to promote the germination of Cynomorium cynomorium seeds. The ratio of the sodium chloride and the strigolactone synthetic analog GR24 can be 100-400mmol sodium chloride and 10-8-10-10mol strigolactone synthetic analog GR24. The method of the invention can quickly and efficiently break the dormancy of Cynomorium cynomorium seeds and make the germination rate reach 9-22% within 40 days, and the germination is orderly, which greatly saves the time of Cynomorium cynomorium breeding.
Owner:INNER MONGOLIA UNIVERSITY
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