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Kit for detecting inheritance gene restoring capability

A technology of genetic genes and kits, applied in the fields of molecular biology and medicine, can solve problems such as the decline of repair ability, the weakening of transcription factor complex performance, and the decline of transcription factors

Inactive Publication Date: 2008-08-13
XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A number of current studies have shown that the polymorphism of this site will weaken the performance of the transcription factor complex that ERCC2 participates in, thereby reducing the ability to repair DNA damage, and the ability of transcription factors will also decrease at the same time, resulting in a series of Occurrence of diseases such as lung cancer, prostate cancer, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1. Use of detection kits

[0030] Step 1: Extraction of DNA template

[0031] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption.

[0032] Step 2: Real-time quantitative PCR reaction

[0033] Use a fluorescent quantitative PCR detection kit for detecting individual genetic repair ability, which contains the following primer pairs and fluorescent probe pairs:

[0034] Sense primer 1: 5'-TTTGCCATTCACTGTGTTGG-3' (SEQ ID NO: 1)

[0035] Antisense primer 1: 5'-ATCCTTGCTGCTATCATCA-3' (SEQ ID NO: 2)

[0036] Sense primer 2: 5'-TTAGAAGGTGACAGTGACCA-3' (SEQ ID NO: 3)

[0037] Antisense primer 2: 5'-TCTCAACCCTACTCACTCA-3' (SEQ ID NO: 4)

[0038] Sense primer 3: 5'-TAACTGGCATCTTCACTTCT-3' (SEQ ID NO: 5)

[0039] Antisense primer 3: 5'-TCCAGATTCCTGGCATTGC-3' (SEQ ID NO: 6)

[0040] Sense primer 4: 5'-ATCAAAGAGACAGACGAGCA-3' (SEQ ID NO: 7)

[0041] Antisense primer 4: 5'-TCAGGAAGCCCAGGAAAT-3' (SEQ ID NO: 8)

[0042] Sense primer 5: 5'-ACTCAG...

Embodiment 2

[0063] Example 2. Services for testing people's ability to repair individual genetic genes

[0064] Step 1: DNA Extraction

[0065] The physicians in the laboratory department of the hospital instructed the subjects to use oral swabs to sample oral epithelial cells, and the silica gel adsorption method was used to extract DNA from oral epithelial cells.

[0066] Step 2: Genotyping Assays

[0067] Using the kit provided by the present invention, the SNP site No. rs1136410 on the poly ADP ribosyltransferase gene of the genomic DNA of the subject, the SNP site No. rs1799782 and No. rs25487 on the human X-ray staggered complementary repair 1 gene, and the excision repair compound The SNP sites rs1799793 and rs13181 on the object 2 gene were detected by real-time quantitative PCR to determine the genotypes of these five SNPs sites.

[0068] Step 3: Analysis of individual genetic repair ability

[0069] Through the analysis of the SNPs genotype of the tested person, an analysis r...

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Abstract

The invention discloses an agent box for detecting idiogenetics gene repair capacity. The agent box comprises specificity primer pair and specificity fluorescent detecting probe pair for detecting synchronously number rs1136410 SNP site on multi-poly ADP ribose transferase gene (PARP1), number rs1799782 and rs25487 SNP site on human beings X ray intervein complementation repair gene (XRCC1), number rs1799793 and rs13181 on cutting repair complexes gene (ERCC2), general component for detecting fluorescent definite quantity PCR etc.. The agent box of the invention assesses idiogenetics gene repair capacity by detecting synchronously mononucleotide polymorphism site gene type correlative closely to idiogenetics gene repair capacity.

Description

technical field [0001] The present invention relates to the fields of molecular biology and medicine. More specifically, the present invention relates to a kit for detecting individual genetic gene repair ability, by simultaneously detecting the poly ADP ribosyltransferase gene (PARP1) closely related to genetic gene repair ability , human X-ray staggered complementary repair gene 1 (XRCC1), excision repair complex 2 gene (ERCC2) single nucleotide polymorphism site genotypes to evaluate individual genetic gene repair ability. Background technique [0002] In the process of genomic nucleic acid replication, there are complex mechanisms in cells to ensure that genetic information is replicated correctly, because the genetic conservation of DNA is the main factor to maintain the relative stability of species. However, under the influence of various factors in the internal and external environment of organisms, DNA damage, also known as DNA mutation, will also occur. Most of th...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 冯哲民邹祖烨
Owner XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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