35 results about "XRCC1" patented technology

The invention discloses a kit for testing the genetic risk of pancreatic cancer, which comprises specific primer pairs for testing the single nucleotide polymorphic locus genotypes of a 5,10-methylenetetrahydrofo late reductase gene (MTHFR), a xeroderma pigmentosum complementary set C gene (XPC), X-ray repair cross-complementing gene 1 (XRCC1) and a thymidylate synthase gene (TS) as well as specific fluorescent probe pairs, fluorescent quantitative PCR regular components, PCR reaction components and the like. The kit of the invention estimates the individual genetic risk of pancreatic cancer by synchronously testing the polymorphic locus genotypes of the genes closely related to the genetic risk of pancreatic cancer.

The invention relates to a kit for predicting lung cancer metastasis, and a usage method of the kit. The kit comprises a DNA library kit which comprises probes of multiple genes, and the multiple genes comprise: high-risk genes: BRCA1, BRCA2, PALB2, ATM, ATR, MUTYH, EMSY, ERCC4, RAD51, PARP1 and XRCC1; and low-risk genes: ATRX, BRIP1, FANCA, FANCB, FANCC, FANCD2, FANCE, FANCF, FANCG, FANCI, FANCL, FANCM, FANCO, FANCP, MDM2, MDM4, MLH1, NPM1, PP2R1A, PRKDC, RAD50, STAG2, XRCC5, XRCC6. Through performing related mutation detection to the related genes of DNA repair system signal paths, lung cancer metastasis can be rapidly and conveniently judged and predicted by combining a special rating mechanism.

The invention provides a non-invasive detection kit for susceptibility gene of basal cell carcinoma. The kit comprises a specific primer and a DNA sequencing primer which are used for detecting the genotypes of a Mn-SOD gene, a P53 gene and an XRCC1 (X-ray repair cross-complementing group 1) gene, a PCR (Polymerase Chain Reaction) reaction system, a PCR product purification system and a DNA sequencing reaction system, etc. The kit disclosed by the invention is used for evaluating the suffering risk level and degree of basal cell carcinoma in people by detecting the genotypes of three single nucleotide polymorphic sites closely related with occurrence of basal cell carcinoma, and finally guiding Chinese people to pointedly prevent occurrence of basal cell carcinoma from gene dimension according to the gene detection results of a detected person, thereby reducing the morbidity risk rate of basal cell carcinoma. A sample used in the detection kit provided by the invention is a mouth mucosa cell, and a collection method is free of pain and non-invasive, and can prevent cross infection.

Evofosfamide (TH-302) or an analog thereof has a specific inhibitory effect on a cell having a specific gene mutation, in particular on a DNA repair-damaged cell. The cell or tissue has at least one or more gene mutations in BRCA1, BRCA2, FANCD1, FANCD2, ATM, ATR, CHEK1, CHEK2, CTP, BARD1, BRIP1, PALB2, RAD51D, RAD51C, RAD52, RAD54, RAD55, RAD57, FAM175, NBN, Rad50, MER11, p53, NBS1, XRS2, XRCC2,XRCC3, ERCC1, ERCC2, ERCC3, ERCC4, XRCC1, Ku80, MHS6, MGMT, PARP, and ERCC5. For this, a medical use of evofosfamide (TH-302) or an analog thereof in treating tumors or cancer in a cancer patient having the specific gene mutation(s) is provided.

The invention discloses an XRCC1 (X-Ray Repair Cross-Complementing Gene 1) gene mutation detection liquid-phase chip and specific primer. The XRCC1 gene mutation detection liquid-phase chip mainly comprises an ASPE primer, microspheres and an amplification primer, wherein the ASPE primer consists of a tag sequence at a 5'-end and a specific primer sequence for a gene mutation site at a 3'-end; the specific primer sequence is SEQ ID NO. 7 and SEQ ID NO. 8 for an A1196G site, SEQ ID NO. 9 and SEQ ID NO. 10 for a C580T site, and SEQ ID NO. 11 and SEQ ID NO. 12 for a G839A site; the microspheres are coated by an anti-tag sequence. The agreement rate of the detection result of the XRCC1 gene mutation detection liquid-phase chip and that of a sequencing method is as high as 100%. Independent or parallel detection on a wild type and a mutation type of multiple mutation sites can be achieved.

The invention discloses a rapid testing method for main polymorphism site of repair protein gene (XRCC1) of human ribonucleotide. According to designed primers of genetic DNA sequence of human chromosome XRCC1, the invention employs polymerase chain reaction (PCR) specific technique to amplify a fragment of DNA sequence respectively including mutation sites of R194W, R280H and R399Q, respectively uses a pair of molecular fluorescent probes to be hybridized with amplified product. Whether a sample includes mutation of R194W, R280H and R399Q is tested by real-time detection of a fluorescent PCR instrument to corresponding specific fluorescent intensity alternation of reaction tubes or by direct detection of specific fluorescent intensity of reaction tubes via a fluorescence spectrophotometer after PCR. The method is convenient and rapid, high in specificity, economic and practical, which is applicable for rapid detection of large-scaled samples. The invention further provides kits applicable for clinical application.

Glufosfamide or analogues thereof have specific inhibitory effects on cells with specific gene variations, specifically, DNA repairs damaged cells, the cells or tissues at least have one or more genemutations of BRCA1, BRCA2, FANCD1, FANCD2, ATM, ATR, CHEK1, CHEK2, CTP, BARD1, BRIP1, PALB2, RAD51D, RAD51C, RAD52, RAD54, RAD55, RAD57, FAM175, NBN, Rad50, MER11, p53, NBS1, XRS2, XRCC2, XRCC3, ERCC1, ERCC2, ERCC3, ERCC4, XRCC1, Ku80, MHS6, MGMT, PARP or ERCC5. The analogues are esters obtained by esterification reaction of one or more hydroxyl groups in glufosfamide molecules with organic acid and inorganic oxyacid; esters obtained by esterification reaction of one or more hydroxyl groups in glufosfamide molecules with amino acid; and salts obtained by reaction of glufosfamide molecules withan acid. Therefore, provided is medical uses of glufosfamide or the analogues thereof in treatment of tumors and cancer diseases of cancer patients with specific gene variations.

The invention provides a thyroid cancer susceptibility gene detection kit, which comprises specific primers for detecting XRCC1 gene Arg399Gln locus and hMSH2 gene 13th exon sequence, DNA sequencing primers, a PCR reaction assembly and the like. According to the present invention, the thyroid cancer susceptibility risk levels of subjects are evaluated by detecting the information of the genes XRCC1 and hMSH2 closely related to the occurrence of thyroid cancer, and according to the gene detection results of each subject, the subjects can be guided with the targeted prevention of the occurrenceof thyroid cancer so as to reduce the incidence of thyroid cancer; and the detection result is accurate and reliable, and the method is easy to popularize.

The invention discloses a set of primers capable of simultaneously detecting ERCC4 and XRCC1 genetic polymorphism. The primers include a PCR amplification primer and an SNaPshot PCR primer, and simultaneous detection loci include ERCC4 rs1799801 and XRCC1 rs25487; compared with conventional individual amplification performed on multiple loci, amplification programs can be minimized, amplificationcosts can be reduced, and high sensitivity, accuracy and precision can be guaranteed by applying the primers to the selection of clinical chemotherapy drugs, and therefore, the reliability of a methodcan be ensured; and the primers are helpful for doctors to correctly select drugs and treatment schemes for patients, so that the survival rates of cancer patients can be increased.

The invention relates to a primer group, reagent and / or kit and system for detecting lung cancer chemotherapy related genes, and application. The primer group contains primers for detecting gene mutation of ERCC1, MTHFR, GSTP1, XRCC1, DYNC2H1, ABCB1, CYP2C8*3, TP53, NQO1, CBR3, SOD2, CYP2C19, UGT1A1*6, TYMS, NT5C2 and CDA. The primer group, the reagent and / or the kit, the system and the application have the advantages of high accuracy, high specificity, high sensitivity, good precision and the like, and meanwhile, further have the advantages of sample saving, short detection period, easy operation, convenient analysis and the like.