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Pancreatic cancer genetic test kit

A kit and technology for pancreatic cancer, applied in the field of genetic detection kits for pancreatic cancer, can solve problems such as increased risk of pancreatic cancer

Inactive Publication Date: 2009-12-23
XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that those who carry the 3Rc / 3Rc genotype have a significantly increased risk of pancreatic cancer

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1. Use of detection kits

[0036] Step 1: Extraction of DNA template

[0037] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption.

[0038] Step 2: Electrophoretic detection and typing

[0039] Using the electrophoresis detection kit in the detection kit, the reaction system has a total volume of 25 μl, including 1 μl of DNA template with a concentration of 12.5 ng / μl, 2.5 μl of 10X PCR reaction buffer, 0.2 μl of 25mM dNTP mixture, and 1.5 μl of 25mM MgCl2 solution , 0.125 μl of 5 units / μl Taq DNA polymerase, 0.25 μl of each primer for 20 μM electrophoresis detection primer pair, 18.675 μl of deionized water.

[0040] The reaction was carried out on a PCR amplification instrument, and the reaction conditions were 94° C. for 12 minutes, and 30 cycles of 94° C. for 30 seconds, 60° C. for 30 seconds, 72° C. for 30 seconds, and then 72° C. for 10 minutes.

[0041] Then use electrophoresis technology to perform agarose gel electrophoresis t...

Embodiment 2

[0049] Example 2. Application of detection kits for individual pancreatic cancer genetic susceptibility testing services

[0050] Step 1: DNA Extraction

[0051] The physicians in the laboratory department of the hospital instructed the subjects to use oral swabs to sample oral epithelial cells, and the silica gel adsorption method was used to extract DNA from oral epithelial cells.

[0052] Step 2: Genotyping Assays

[0053] Use the kit provided by the present invention to carry out PCR amplification and electrophoresis detection on the XPC gene and the SNP site on the TS gene of the subject's genomic DNA, and perform PCR amplification and electrophoresis detection on the C677T SNP site on the MTHFR gene and the Arg194Trp SNP site on the XRCC1 gene. Real-time quantitative PCR detection was performed to determine the genotypes of the four SNP sites.

[0054] Step 3: Analysis of Individual Genetic Susceptibility to Pancreatic Cancer

[0055] Through the analysis of the SNP g...

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Abstract

The invention discloses a kit for testing the genetic risk of pancreatic cancer, which comprises specific primer pairs for testing the single nucleotide polymorphic locus genotypes of a 5,10-methylenetetrahydrofo late reductase gene (MTHFR), a xeroderma pigmentosum complementary set C gene (XPC), X-ray repair cross-complementing gene 1 (XRCC1) and a thymidylate synthase gene (TS) as well as specific fluorescent probe pairs, fluorescent quantitative PCR regular components, PCR reaction components and the like. The kit of the invention estimates the individual genetic risk of pancreatic cancer by synchronously testing the polymorphic locus genotypes of the genes closely related to the genetic risk of pancreatic cancer.

Description

technical field [0001] The present invention relates to the fields of molecular biology and medicine, more specifically, the present invention relates to a kit for detecting the genetic susceptibility of individual pancreatic cancer, by simultaneously detecting the reduction of 5,10-methylenetetrahydrofolate closely related to pancreatic cancer Single nucleotide polymorphisms in enzyme gene (MTHFR), xeroderma pigmentosa complementation group C gene (XPC), X-ray staggered complementation repair gene 1 (XRCC1) and thymidine synthase gene (TS) type to assess individual genetic susceptibility to pancreatic cancer. Background technique [0002] Pancreatic cancer has a high degree of malignancy, is difficult to detect early, metastasizes early and quickly, and has a poor prognosis. According to statistics, only 12% to 15% of cases can undergo radical surgery after diagnosis, and the 5-year survival rate after operation is only 0-2%, while more than 90% of patients die within 1 ye...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N21/64
Inventor 冯哲民邹祖烨
Owner XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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