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242 results about "Method screening" patented technology

Method of screening reprogramming factor, reprogramming factor screened by the method, method of using the reprogramming factor, method of differentiating undifferentiated fused cells and method of constructing cell, tissues and organs

InactiveUS20050130144A1Rejection reaction in the recipient against the transplantation graft can be more efficiently suppressedCompound screeningApoptosis detectionMethod screeningSomatic cell
By exposing somatic cells to a component derived from ES cells to act on somatic cells and detecting the activity thereof, the reprogramming agent can be screened. Further, by exposing somatic cells to a component including a reprogramming agent or an isolated reprogramming agent, the somatic cells can be reprogrammed. Furthermore, according to the present invention, since the tetraploid cells have proliferating capability and pluripotency, such cells can be differentiated and the cells, tissues or organs, which can be used for transplantation, can be produced.
Owner:REPROCELL +1

Preparation of efficient phenol-degrading halophilic bacteria and application thereof

InactiveCN101768564AOptimal hydraulic retention timeThe performance advantage of reducing phenol is obviousBacteriaTreatment using aerobic processesActivated sludgeBatch processing
The invention discloses a preparation of efficient phenol-degrading halophilic bacteria and an application thereof, belonging to the technical field of treatment of waste water and sewage. In the invention, halophilic bacteria in the sewage or sludge are taken as a bacteria source; a gradient domestication method is adopted to screen efficient phenol-degrading halophilic bacteria which have strong degradation capability, can adapt to higher salt concentration and can grow well by taking phenol as the sole carbon source in the phenol-containing waste water with the salt content of 5-6% and thephenol concentration of 100-500mg/L; under the environment of high salt and high phenol, the phenol-degrading performance of the mycelium has obvious advantages over that of the traditional activatedsludge method. The strain can be applied to phenol-degrading treatment of waste water to determine the optimal water power retention time and the optimal bacteria input amount and batch processing time, and the operational conditions can be optimized through orthogonal experiments. The screening of the efficient phenol-degrading halophilic bacteria has high application value, and can provide a reference for the removal of phenol in the oil extraction waste water and the industrial treatment of the phenol-containing wastewater with high content of salt in petrochemistry industry.
Owner:BEIHANG UNIV

Method for modifying recycled aggregate based on aerobic basophilic mixed bacterium mineralization deposition

ActiveCN107226630ANo negative impact on physical and mechanical propertiesImprove surface compactnessSolid waste managementMicroorganismsScreening methodOxygen
The invention discloses a method for modifying recycled aggregate based on aerobic basophilic mixed bacterium mineralization deposition. The method comprises I, screening aerobic basophilic mixed bacteria and II, modifying recycled aggregate. Through a bacterial screening method, aerobic basophilic mixed bacteria having good practicability to the survival environment are screened. In the environment with enough calcium sources, oxygen and water, the mixed bacteria realize mineralization deposition of a large amount of calcium carbonate precipitates through metabolism. Through the excellent mineralization deposition capacity of the mixed bacteria, cracks and holes on the surface of recycled aggregate are repaired. In modification of the recycled aggregate, the desired mixed bacteria are screened and then are cultured, then the bacterium solution is adsorbed by the surface of recycled aggregate through a vacuum absorption method, and a calcium lactate solution is sprayed to the recycled aggregate to cure the recycled aggregate so that the surface defects of the recycled coarse aggregate are repaired. The method can effectively improve the physical and mechanical properties of the recycled coarse aggregate.
Owner:山西耀辉实业发展股份有限公司

Method for identifying source constituent in meat product by micro-satellite labeling technique

The invention relates to a method which uses microsatellite marking technology for identifying the animal origin ingredients in meat products, belonging to the biological high-tech field. The use of the microsatellite marking technology for identifying the origin ingredients of cattle, sheep, pigs, dogs, chickens and ducks in the meat products comprises the extraction of genomic DNA in meat products, the conducting of PCR reaction, the PCR product detection and primer screening, and the identification of samples. The method selects microsatellite marks with specific property among the types and universal property in the type, designs specific primers, establishes PCR detection methods, and identifies the origin ingredients of cattle, sheep, pigs, dogs, chickens and ducks in the meat products according to the size and the availability of amplification fragment. The invention has the advantages of rapidity, simplicity, accuracy, strong specificity and high sensitiveness. The invention is suitable for being promoted and used for detecting multitudinous samples. The invention also has great significance for preventing the mad cow disease from being spread into China and the illegal businessmen from concealing the commodity elements, and ensuring national security and the health of people.
Owner:NANJING AGRICULTURAL UNIVERSITY

Screening and construction method for composite microbial system for rapidly degrading rice straw and composite microbial system

InactiveCN101899395AImprove decomposition effectMake up for the lack of decayFungiBacteriaMicroorganismDecomposition
The invention discloses a screening and construction method for a composite microbial system for rapidly degrading rice straw and a composite microbial system, which belong to the technical field of microbial screening methods. The method comprises the following steps of: (1) constructing a strain pond; (2) preparing a solid culture medium; and (3) screening and constructing the composite microbial system. The composite microbial system screened by the method comprises bacteria and fungi. The method and the composite microbial system have the advantages that: the composite microbial system is obtained by screening under the condition of the solid culture medium, comprises the bacteria and the fungi and has better decomposition effect; the composite microbial system RSS-4 is screened under the condition of unsterilized solid culture medium; the screened composite microbial system has better decomposition effect at the intermediate temperature, namely the temperature of 22 DEG C; and the defect that the composite strain can only decompose at the temperature of 28 DEG C existing in the prior art is effectively overcome.
Owner:INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI

Method for screening new internal reference molecules suitable for cervical tissue micro RNA real-time fluorescence quantitative PCR research

The invention discloses a method for screening new internal reference molecules suitable for cervical tissue micro RNA real-time fluorescence quantitative PCR research. The method comprises the following technical steps: screening human Micro RNA probes by using a Micro RNA chip, and selecting Micro RNAs with constant normal tissue expression and high expression abundance of cervical cancer and cervix as candidate internal references; selecting a normal cervical epithelial tissue and a cancerous cervical tissue as clinical cervical tissue specimens respectively, and verifying the specimens by using fluorescence quantitative PCR; and analyzing data by using two special internal reference analysis software comprising geNorm software and Norm Finder so as to discover the internal reference molecules suitable for cervical tissue Micro RNA research. The internal reference molecules screened and verified by using the method of the invention can avoid possible differences of different cervical tissue specimens on the yield, quality and reverse transcription efficiency of the RNA, well correct and standardize the data for real-time fluorescence quantitative PCR detection, and improve the accuracy and the reliability of the search.
Owner:ATTACHED OBSTETRICS & GYNECOLOGY OSPITAL MEDICALCOLLEGE ZHEJIANG UNIV

SNP (single-nucleotide polymorphism) locus related to heat resistance of Paralichthys olivaceus and application thereof

The invention relates to an SNP (single-nucleotide polymorphism) locus related to heat resistance of Paralichthys olivaceus. The SNP locus is 47th / 48th site (the base is AA or A / DEL), 65th site (the base is T or C), 185th site (the base is C or T), 260th site (the base is C or T), 305th site (the base is T or A), 600th site (the base is T or C) or 815th site (the base is C or T) of HSP70 gene with the nucleic acid sequence of SEQ ID NO:1. The SNP locus is used for screening the heat-resistant individual / family of Paralichthys olivaceus. The heat-resistant family is screened by a molecular marker process by using the heat shock protein gene HSP70; the hereditary characters of all the families can be evaluated on the molecular level, and the mutant site of the genome can be clearly identified; and the relevance between the mutant site and heat resistance is analyzed by combination of the heating experiment to perform reasonable judgment on the heat-resistant molecular mechanism. Compared with the traditional breeding method, the method provided by the invention has the advantages of higher purposiveness and lower cost, and can obviously shorten the breeding time.
Owner:OCEAN UNIV OF CHINA

Method for constructing composite bacteria system for decaying maize straws

The invention relates to a method for constructing a composite bacteria system for decaying maize straws, which belongs to the technical field of a microorganism screening method. The method for constructing the composite bacteria system for decaying the maize straws comprises the following steps of: (1) performing enrichment culture on bacteria; (2) preparing a solid culture medium; (3) performing inducing culture of nutrient limitation; (4) performing inducing culture of gradient temperature reduction; and (5) performing successive transfer culture to obtain the composite bacteria system CSS-1 for decaying the maize straws. The composite bacteria system screened by the method comprises bacteria, fungus and actinomycetes. The method has the advantages that: the composite bacteria system which comprises the bacteria, the fungus and the actinomycetes is obtained by screening under the condition of the solid culture medium; and the decaying effect is good.
Owner:INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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