32 results about "Intestinal type" patented technology

Objective methods for detecting and diagnosing intestinal-type gastric cancers are described herein. Also described is method for predicting the presence or absence of lymph node metastasis (i.e., identifying the metastatic phenotype). In one embodiment, the diagnostic method involves the scoring of gene expression profiles that discriminate between lymph node positive tumors and lymph node negative tumors. The predictive score calculated acts as diagnostic indicator that can objectively indicate whether a sample tissue has the metastatic phenotype. The present invention further provides methods of diagnosing intestinal-type gastric cancer in a subject, methods of screening for therapeutic agents useful in the treatment of intestinal-type gastric cancer, methods of treating intestinal-type gastric cancer and method of vaccinating a subject against intestinal-type gastric cancer.

The invention discloses an intestinal metagenome feature as a type-2 diabetes acarbose curative effect selection marker. The intestinal microorganism metagenome feature is the bacteroides intestinal pattern. By using the intestinal pattern concept for the first time, it is found that type-2 diabetes patients with different intestinal parasitic bacterium groups make remarkably different treatment responses to diabetes hypoglycemic drugs acarbose, and therefore before the drugs are used, the intestinal patterns of the patients can be distinguished, people with the optimal curative effect are selected, and whether acarbose is suitable for conducting diabetes treatment on the individual type-2 diabetes patient or not is judged. In addition, the conventional distinguishing of the intestinal patterns is achieved through DNA sequencing of parasitic bacteria in excrement or PCR amplifying, and the intestinal patterns can be well distinguished under the condition of a base line through bile acid composition especially secondary bile acid; the intestinal patterns can be identified through markers in blood, and the marker becomes a marker for diagnosis.

the invention discloses a verifying method of gastrointestinal gastric cancer and differential degree and specific agent box, which adopts expressive level of at least one gene in the list 1, list 2, list 3 and list 4.

The invention discloses a detection method for a human intestinal flora. The method comprises the following steps: firstly, collecting a large number of human excrement samples, processing each excrement sample to obtain intestinal flora information, and then constructing an intestinal pattern and a flora diversity distribution trend chart according to the intestinal flora information of each sample; and determining the intestinal pattern of a to-be-detected sample according to intestinal flora information of the to-be-detected sample and the intestinal pattern constructed in a system, and comparing the intestinal flora information of the to-be-detected sample with the flora diversity distribution trend chart in the system to obtain a detection result of the to-be-detected sample, therebyrealizing detection of the intestinal flora. According to the invention, the detection of the human intestinal flora can be realized, so help and reference are provided for further applying the detection of the human intestinal flora to obesity detection and research. The invention further provides a detection device for the human intestinal flora and a storage medium.

The invention discloses a construction method of a Prevo intestinal type in-vitro simulation model. The fresh feces of the Prevo-intestinal healthy volunteers are diluted with PBS into a fecal suspension by using an automatic intestinal flora in-vitro simulation model apparatus, and the fecal suspension is inoculated into a modified VI medium, and fermentation culture is carried out under conditions of 37 DEG C, pH of 5.2-5.8, magnetic stirring speed of 150 rpm, and supplemented addition with a medium at a rate of 330 ml / 12 h to obtain the Prevo intestinal type in-vitro simulation model. The method finds an important nutritional condition for simulating the Prevo intestinal type in-vitro, explores the physical and chemical conditions in the continuous fermentation process, and can increasethe proportion of Prevo bacteria in a broth to 40% to 80%, and the correlation coefficient between the microbial population in the broth and an original stool sample is about 80%, so that the Prevo intestinal type can be stably simulated in vitro.

The invention relates to a method of using an S1P receptor conditioning agent to prevent and / or cure stomach and intestine organization damages caused by ionising radiation, concretely to individually use, or combined with other treatment methods, the S1P receptor conditioning agent to prevent and / or cure intestinal radiation sickness and radiation enteritis caused by tumour radiation treatment.

Methods and compositions for detecting tumor origin are disclosed. In certain embodiments, the origin is determined by detecting expression levels of pan-epithelial membrane mucin (MUC1), intestinal-type secretory mucin (MUC2), cytokeratin 17 (CK17), or a combination thereof. Also disclosed are methods for aiding in the determination of an appropriate course of treatment for a subject with a tumor, or for predicting a therapeutic outcome of a subject, based on the expression levels of MUC1, MUC2, CK17, or a combination thereof. Kits for use in each of these methods are also provided.

The invention discloses a hair ball removing nutrient solution and a preparation method thereof. The hair ball removing nutrient solution is prepared from the following components: plantain seed shell, fried semen raphani, fructus cannabis, cortex magnoliae officinalis, plantain seed shell cellulose and black fungus. Pet cats are used to lick their own hair to clean themselves, but at the same time, the pet cats eat a lot of dead hair which is accumulated in the stomach and intestine, and if the pet cats do not spit out in time, intestinal tracts may be blocked. Therefore, a formula is provided, the plantain seed shell is used as a monarch material to relax bowel, absorb water in small intestines to form pasty blocks, improve the lubricating conditions of excrement and intestines and promote the defecation speed; fried semen raphani, fructus cannabis and cortex magnoliae officinalis are used as ministerial drugs to promote digestion and relieve stasis; the semen plantaginis cellulose and the black fungus are also known as intestinal tract cleaners, and the black fungus contains insoluble dietary fibers to help the monarch drug to discharge hair smoothly, so that the situation that the stomach is hurt and discomfort are caused due to the fact that partial hair discharge products in the market vomit from the mouth to discharge hair after the catnip is orally taken is avoided, and the effects of relaxing bowel, promoting digestion, relieving indigestion and discharging hair are achieved by combining all the raw materials.

The invention provides application of propylene glycol to medicine for preventing intestinal type radiation disease and radiation enteritis. According to the application disclosed by the invention, the propylene glycol is firstly applied to the field of radiation protection research, and the research proves that compared with former prevention application, the propylene glycol has an obvious experimental therapeutic effect on acute radiation injury of intestinal tracts and has curative effects on intraperitoneal injection and oral administration; moreover, a radiation protection mechanism of the propylene glycol is possibly related with removal of hydroxyl radicals through the propylene glycol and alleviation of intestinal cell DNA injuries. At present, the phenomenon that gastrointestinaltissue injuries or the radiation enteritis occurs when the propylene glycol is applied to prevention of ionizing radiation or tumor radiotherapy is not reported yet.

The invention discloses an automatic typing method based on human intestinal flora. According to the invention, clustering results are grouped by adopting a LEfSe mode, Biomarker screening is conducted, and then specific intestine types are determined; obtained results are comprehensive and comprise involved clustering graphs, Biomarker screening and intestinal type boxplot graph display; all theanalysis results can be automatically sorted out, summarizing and statistics are automatically conducted on the results after each step of analysis is completed, and visualization is realized; and alloperation steps can be traced back, so error query is facilitated, and if errors are analyzed and reported, corresponding error reporting log information can be obtained.

The invention discloses an application of plant polysaccharide in preparing medicine, food, health care product or food additive for intestinal type-related hyperuricemia, wherein the plant polysaccharide is inulin and / or psyllium seed husk, and the intestinal type-related hyperuricemia is prevotella intestinal type hyperuricemia and / or bacteroides intestinal type hyperuricemia. The invention provides the new application for two vegetable polysaccharides comprising inulin and psyllium seed husks, namely, the application in treating or preventing intestinal type-related hyperuricemia, in particular to prevotella intestinal type hyperuricemia and / or bacteroides intestinal type hyperuricemia. Discovery of the new application has a good guiding effect on implementation of individualized diagnosis and treatment, and has important significance on clinical application and deep research of vegetable polysaccharides.

The invention belongs to the field of traditional Chinese medicine processing, and discloses a preparation method of an excrement inoculum substitute in a preparation process of rulvis glycyrrhizae praeparata. The preparation method comprises the following steps of (1) preparing an excrement inoculation liquid, namely, adding 0.1 M of sterilized and anaerobic treated PBS (Phosphate Buffer Solution) with the pH value of 7.0 into excrement, carrying out uniform vortex mixing, passing through a filtering membrane, and taking filtrate to obtain the excrement inoculation liquid; (2) determining the enterotypes of the intestinal microecology of the excrement inoculation liquid; (3) inoculation, namely, adding the excrement inoculation liquid into a fermentation culture medium in a fermentation tank, performing anaerobic fermentation, and performing in-vitro culture of enteric microorganisms to obtain fermentation liquid containing the enteric microorganisms; (4) continuously fermenting for 10-12 days, and collecting fermentation liquid when florae in the fermentation tank are stable; and (5) wrapping licorice powder with a semipermeable membrane (with the aperture of 2-5 microns), immersing the licorice powder in the bacterial liquid, fermenting at 37 DEG C for 3-14 days, taking out and drying. According to the method, the excrement is selected to be subjected to appropriate enlarged culture according to different enterotypes, the excrement inoculum which is consistent and controllable in quality is produced, and a feasible and operable production scheme is provided for standardized production of the pulvis glycyrrhizae praeparatus.

The invention provides a simulation method of chronic atrophic gastritis lesion. The simulation method comprises the following steps: (1) adopting a metamorphic lesion stage as a simulation object; (2) adopting a simulation form of spasmolysis polypeptide expression metaplasia; and (3) conditionally knocking out a GRIM-19 gene from gastric mucosa wall cells. Initialized response after gastric mucosa damage is successfully simulated, IM and even gastric cancer can be developed under continuous stimulation of chronic inflammation, simulation of pathology formation provides a basis for research on early prevention and control of intestinal gastric cancer and effective restraint of precancerous lesions of gastric cancer, a research basis can be provided for screening and development of drugs for preventing and treating CAG, and an important experimental tool is provided for anti-inflammatory and anti-cancer drug tests. The method has the characteristics of high simulation degree, stability, durability, specific phenotype and high efficiency.

The purpose of the present invention is to provide a method whereby it is possible to promptly diagnose the type of pancreatic tumor. Specifically, the present invention pertains to an examination method for determining the type of pancreatic tumor. Said method detects, in the pancreatic fluid sample from a subject, the degree of methylation in a 5'-untranslated region or a region containing a coding region and the 5'-untranslated region of a gene coding mucin core protein, and uses the degree of methylation as an index to classify the pancreatic tumor into a type of pancreatic tumor selected from among pancreatic cancer, gastric type of intraductal papillary mucinous neoplasm, intestinal type of intraductal papillary mucinous neoplasm, and pancreaticobillary type of intraductal papillary mucinous neoplasm.

The invention relates to a benzamide derivative as well as a preparation method and medicinal application thereof. Specifically, the invention relates to a benzamide derivative shown by a general formula (I), a preparation method thereof and a pharmaceutical composition containing the benzamide derivative, and an application of the benzamide derivative as a therapeutic agent and particularly as an intestinal 2B-type sodium phosphate cotransporter (Npt2b) inhibitor and an application thereof in preparing a medicine for treating and / or preventing hyperphosphatemia, wherein the definition of each substituent in the general formula (I) is the same as that in the specification.

The invention relates to a crystal form of an intestinal type-2B sodium phosphate synergistic transportprotein inhibitor and a preparation method thereof, in particular to a crystal form II of N<1>-methyl-N<1>-(2-morpholine ethyl)-N<3>-(3-(4-phenylethyl phenylcarbamyl)-4,5,6,7-tetrahydrobenzo[b]thiophene-2-yl)isophthalamide sulfate (a compound shown in formula (I)) and a preparation method. The crystal form II of the compound in formula (I) obtained in the invention has good chemical stability and crystal form stability and can be well applied to the clinical treatment. (Shown in the description).

The invention discloses an intestinal microecology differential bacteria classification processing method and an intestinal health assessment method, and the method comprises the following steps: S10, collecting a sample set A which at least comprises n samples composed of an unhealthy group sample DG and a control group sample CG; step S11, converting the digital feature of each sample from absolute abundance to relative abundance; step S12, dividing all samples in the sample set A into k groups of intestinal types according to the similarity of absolute abundance, marking an intestinal type set obtained after classification as AB, then separating an unhealthy group sample DG and a control group sample CG in the intestinal type set AB, marking the unhealthy group sample set as DG *, and marking the control group sample set as CG *; and S13, calculating the relative abundance difference between the unhealthy group sample set DG * and the control group sample set CG *, and sorting the relative abundance difference to obtain difference characteristics DF corresponding to the k groups of intestinal types. The invention not only can effectively extract high-dimensional sample features with a large abundance change range, but also is higher in anti-interference performance.

The invention discloses a method for constructing an in vitro simulation model of Prevot enterotype. The fresh feces of healthy volunteers with Prevot enterotype are diluted with PBS into a feces suspension by using a fully automatic in vitro simulation model device for intestinal flora. Then the feces suspension was inoculated into the improved VI medium, fermented and cultured at 37°C, pH 5.2-5.8, magnetic stirring speed 150rpm, and supplemented with medium at a rate of 330ml / 12h, to obtain Prevot enterotype in vitro Simulation model; the present invention finds the important nutritional conditions for simulating Prevot enterotype in vitro, and explores the physical and chemical conditions in the continuous fermentation process, which can increase the ratio of Prevotella in the fermentation broth to 40%-80%, At the same time, the correlation coefficient between the microbiota in the fermentation broth and the original stool sample reached about 80%, so that the Prevot enterotype could be stably simulated in vitro.

The invention relates to a production method of a medium-temperature sausage product which has the high quality and can be stored at the normal temperature for a long time. The medium-temperature sausage product is prepared from raw material meat, auxiliary materials, spore germination factors and a quality preservation agent through processing; the specific processing technology comprises steps as follows: material selection, pre-processing, meat mincing, meat stuffing production, vacuum filling, spore germination, hot processing, discharging from a furnace and cooling, packaging and medium-temperature sterilization. According to the method, under the coaction of thermal stimulation and the germination factors, spores in the product are effectively killed at the medium temperature of 90-110 DEG C, meanwhile, the quality preservation agent is added, so that vitamins, amino acid and fatty acid in the product are protected, and the product has the high nutritional value and the good flavor.

The invention relates to a feed for culturing rice-fragrance grass carps, and a culturing method of the rice-fragrance grass carps. The feed comprises the following components in weight percentage: 20-30% of algae powder granules, 10-15% of modified soapstone powder, 1-3% of micronutrients, 10-20% of an adhesive, and 40-50% of a mixture of fermented dregs. The feed and the culturing method provided by the invention are adopted to feed the grass carps for 6 months, and the survival rate of the grass carps reaches 100%, and the multiple of weight gaining is 11.07-12.16. Detection indicates that the harvested grass carps are high in protein content, low in fat content, and delicious in meat quality; and meanwhile, in intestinal tracts of the grass carps, the contents of pathogenetic bacteria easily causing enterogastritis are lowered, wherein the pathogenetic bacteria comprise aeromonas hydrophila, aeromonas punctata f. intestinalis, aeromonas caviae, escherichia coli and enterococci, and the contents of probiotics such as lactobacillus and bifidobacteria are increased, so that the risk of enterogastritis occurrence among the grass carps is effectively decreased.

This invention is intended to provide a method capable of diagnosis of pancreatic tumor type at an early stage. More specifically, this invention relates to an examination method for determining pancreatic tumor type comprising detecting the degree of methylation in a 5′-untranslated region or a region comprising a 5′-untranslated region and a translated region of a gene encoding a mucin core protein in a pancreatic fluid sample from a subject, and identifying the pancreatic tumor as being of pancreatic tumor type selected from the group consisting of pancreatic ductal adenocarcinoma, an intraductal papillary mucinous neoplasm of the gastric type, an intraductal papillary mucinous neoplasm of the intestinal type, and an intraductal papillary mucinous neoplasm of the pancreatobiliary type using the degree of methylation as an index value.

The invention discloses an intestinal mucosa improving type selenium supplement, which is formed by chitosan selenium and montmorillonite through mixing according to the mass ratio of 1: (3-7). The invention further discloses a preparation method of the intestinal mucosa improving type selenium supplement, wherein the preparation method comprises the following steps: adding chitosan selenium powder into the montmorillonite suspension liquid (the mass concentration is 2-8%), mixing, adjusting the pH (potential of hydrogen) value to be 2-3.5 with glacial acetic acid, then reacting for 5-8h at 40-80 DEG C, wherein the mass ratio of the chitosan selenium powder and the montmorillonite in the montmorillonite suspension liquid is 1: (3-7); washing the obtained reactant mixture solution with water, filtering, drying at low temperature, and crushing to be 100-300Mu, so as to obtain the intestinal mucosa improving type selenium supplement. The intestinal mucosa improving type selenium supplement is used as a feed additive for beasts and birds or animals, the feed additive has both effect of supplementing selenium and function of enhancing immunity.

The present invention relates to thienocycloalkyl or thienoheterocyclic derivatives, a preparation method thereof and use thereof in medicine. In particular, the present invention relates to thienocycloalkyl or thienoheterocyclic derivatives shown by the general formula (I), a preparation method thereof and a pharmaceutical composition containing the derivatives, and the use thereof as a therapeutic agent, especially as the intestinal type 2B sodium phosphate cotransporter (Npt2b) inhibitor and use thereof in the preparation of drugs for the treatment and / or prevention of hyperphosphatemia, wherein the definitions of the substituents in the general formula (I) are the same as defined in the description.

The invention relates to the field of biotechnology, and discloses two hand, foot and mouth disease virus strains and a dual hand, foot and mouth disease inactivated vaccine prepared from the two virus strains. The human intestinal 71 type virus strain CC-09 with the preservation number CGMCC No.7753 and the Coxsackie A16 virus strain CC-16 virus with the preservation number CGMCC No.7752 were respectively inoculated in Vero cells that were expanded and subcultured. Harvest two kinds of virus culture fluids respectively, concentrate, inactivate and purify to obtain two kinds of virus stock solutions after removing impurities; two kinds of virus stock solutions are sterilized, mixed, and adsorbed with adjuvants to prepare dual inactivated vaccines for hand, foot and mouth disease. The two-combined hand, foot and mouth disease inactivated vaccine of the invention has strong immunogenicity and no cross-inhibition effect between the two viruses, and can effectively prevent the current domestic hand, foot and mouth disease. The two-combined hand, foot and mouth disease inactivated vaccine of the invention has simple preparation process and low production cost, and is suitable for large-scale production of vaccines.