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Thyroid cancer susceptibility gene detection kit

A thyroid cancer, susceptibility gene technology, applied in thyroid cancer susceptibility gene detection kits, medical and biological fields, can solve problems such as affecting tumor susceptibility and weakening base excision repair ability

Inactive Publication Date: 2018-06-15
SHANDONG INT BIOTECH PARK DEV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that the 399th amino acid Arg on the gene is replaced by Gln, which weakens the ability of base excision repair, thereby affecting individual tumor susceptibility

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Step 1: Extract the DNA template, and use a commercial kit to extract the subject's genomic DNA.

[0013] Step 2: PCR amplification reaction

[0014] Use the PCR reaction component in the detection kit, which contains the following primers:

[0015] SEQ.ID NO.1: 5'3'

[0016] SEQ.ID NO.2: 5'3'

[0017] SEQ.ID NO.3: 5'3'

[0018] SEQ.ID NO.4: 5'3'

[0019] The reaction system for PCR amplification is: 10×PCR reaction buffer 2.5ul; 2.5mM dNTP mixture 2ul, 5U / ul Taq enzyme 0.2ul, DNA template about 20ng, 10uM forward primer and reverse primer each 1ul, add deionized Water to 25ul;

[0020] The reaction conditions of PCR amplification were: denaturation and enzyme activation at 95°C for 5 minutes, denaturation at 94°C for 30 seconds, annealing at 55°C for 45 seconds, extension at 72°C for 2 minutes, 35 cycles, and finally extension at 72°C for 10 minutes.

[0021] Step 3: Purification of the PCR amplification product, using a commercial kit to purify the amplification...

Embodiment 2

[0028] Example 2. Services for genetic testing to prevent the onset of thyroid cancer

[0029] Step 1: Sampling and extracting DNA. The physician in the laboratory department of the hospital guides the subject to take samples, and uses a commercial kit for DNA extraction.

[0030] Step 2: Gene sequencing, using the kit provided by the present invention to sequence the genome DNA of the subject.

[0031] Step 3: Risk assessment for high-risk groups of thyroid cancer. Through the analysis of the subject's gene sequence, a risk assessment and analysis report of thyroid cancer susceptibility genes is issued. The kit includes the detection of SNP site information on the XRCC1 and hMSH2 genes and the results of genetic risk assessment. In addition, according to the risk level of the subject, the physician will explain and interpret the thyroid cancer susceptibility gene test report to the subject in detail.

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PUM

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Abstract

The invention provides a thyroid cancer susceptibility gene detection kit, which comprises specific primers for detecting XRCC1 gene Arg399Gln locus and hMSH2 gene 13th exon sequence, DNA sequencing primers, a PCR reaction assembly and the like. According to the present invention, the thyroid cancer susceptibility risk levels of subjects are evaluated by detecting the information of the genes XRCC1 and hMSH2 closely related to the occurrence of thyroid cancer, and according to the gene detection results of each subject, the subjects can be guided with the targeted prevention of the occurrenceof thyroid cancer so as to reduce the incidence of thyroid cancer; and the detection result is accurate and reliable, and the method is easy to popularize.

Description

technical field [0001] The invention belongs to the field of thyroid cancer susceptibility genes, relates to medicine and biotechnology, and specifically relates to a thyroid cancer susceptibility gene detection kit, which evaluates the risk level of thyroid cancer from the genetic level according to the detection results. Background technique [0002] Thyroid cancer accounts for 1% of systemic malignancies. With the exception of medullary carcinoma, the vast majority of thyroid cancers arise from follicular epithelial cells. The incidence of thyroid cancer has a certain relationship with region, race and gender. According to statistics, the incidence of thyroid cancer in China is about 0.8-0.9 / 100,000 for men and 2.0-2.2 / 100,000 for women. [0003] Due to the high degree of malignancy of thyroid cancer, the rapid development of the disease, and the invasion of surrounding tissues, it is often already at an advanced stage when it is diagnosed. Therefore, if the risk of th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/156
Inventor 张博李晓宇朱保伟颜世翠张红梅翟红利张淑敏杨小平
Owner SHANDONG INT BIOTECH PARK DEV
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