Anticancer medical application of evofosfamide
A technology for efsolamide and cancer, applied in the field of efsolamide, which can solve the problem of inability to effectively target tumors for a long time
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[0277] Cell Proliferation Experiment
[0278] Exponentially growing cells were seeded with different concentrations of TH-302 24 hours after inoculation. After drug addition, the plates were incubated at 37°C for 2 hours in an anaerobic chamber (Bactron II), hypoxic chamber (Hypoxystation) or standard tissue culture incubator. After washing, cells were cultured in normoxic complete medium for 72 hours. Viable cells were quantified using AlamarBlue. 50% growth inhibition (IC50) was calculated using Prism software.
[0279] In vitro clonogenic assay
[0280] Cells were seeded 24 hours before starting treatment. Cells were then incubated with drugs for 2 hours at defined oxygen concentrations. At the end of treatment, the washed cells were plated and placed in an incubator for 10 days. For VC-8, UWB1.289 and FANCA strains, cells were plated in triplicate in 6-well plastic plates with 500 to 1,000 cells per well and processed. TH-302 was cultured under hypoxic conditions fo...
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